The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility
Introduction: Breast cancer is the most common malignancy in women worldwide. It is also the second leading cause of cancer death among women after lung cancer. Considering the relationship among plasma folate levels, the level of uracil, and DNA damage in cell division, methyl tetrahydrofolate redu...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2023-02-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| Subjects: | |
| Online Access: | http://sjimu.medilam.ac.ir/article-1-7403-en.html |
| _version_ | 1827998962415566848 |
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| author | Ahmad Hamta Zahra Bozorgi Moghadam |
| author_facet | Ahmad Hamta Zahra Bozorgi Moghadam |
| author_sort | Ahmad Hamta |
| collection | DOAJ |
| description | Introduction: Breast cancer is the most common malignancy in women worldwide. It is also the second leading cause of cancer death among women after lung cancer. Considering the relationship among plasma folate levels, the level of uracil, and DNA damage in cell division, methyl tetrahydrofolate reductase (MTHFR) is a suitable candidate for studies on the susceptibility to cancer, including breast cancer. This study aimed to investigate the relationship between two polymorphisms in the MTHFR gene and the risk of breast cancer among women in Markazi Province, Iran.
Material & Methods: This case-control study investigated the rs1801131 and rs1801133 polymorphisms of the MTHFR gene and breast cancer risk in a large population including 80 patients and 80 healthy women in Markazi Province, Iran, using the PCR-RFLP technique. The specimens were genotyped by agarose gel electrophoresis. Finally, the data were analyzed in SPSS software (version 26).
(Ethic code: Ir.arakmu.rec.1396.25)
Findings: There was a statistically significant difference between the two groups of patients and controls regarding three genotypes of the rs1801133 locus (P=0.006). Moreover, the CT genotype (P=0.001, OR=2.471, 95% CI=1.229-4.965) and total TT and CC genotypes (P=0.000, OR=3.375, 95% CI=1.716-6.637) were significantly associated with the risk of breast cancer. However, the TT genotype (P=0.538) showed a protective role against breast cancer. In contrast, there was no statistically significant difference between the two groups of patients and controls in terms of three genotypes of the rs1801131 locus (P=0.149), which is consistent with the results of some studies.
Discussion & Conclusion: The rs1801133 polymorphism of the MTHFR gene can be used as a marker in clinical prognostic studies related to the risk of breast cancer. |
| first_indexed | 2024-04-10T05:51:45Z |
| format | Article |
| id | doaj.art-55136024caa344a88342ca4f0a5e7557 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-04-10T05:51:45Z |
| publishDate | 2023-02-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-55136024caa344a88342ca4f0a5e75572023-03-04T07:26:17ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352023-02-013062131The Association of the MTHFR Gene Polymorphisms with Breast Cancer SusceptibilityAhmad Hamta0Zahra Bozorgi Moghadam1 Dept of biology, Faculty of science, Arak University, Arak, Iran Dept of biology, Faculty of science, Arak University, Arak, Iran Introduction: Breast cancer is the most common malignancy in women worldwide. It is also the second leading cause of cancer death among women after lung cancer. Considering the relationship among plasma folate levels, the level of uracil, and DNA damage in cell division, methyl tetrahydrofolate reductase (MTHFR) is a suitable candidate for studies on the susceptibility to cancer, including breast cancer. This study aimed to investigate the relationship between two polymorphisms in the MTHFR gene and the risk of breast cancer among women in Markazi Province, Iran. Material & Methods: This case-control study investigated the rs1801131 and rs1801133 polymorphisms of the MTHFR gene and breast cancer risk in a large population including 80 patients and 80 healthy women in Markazi Province, Iran, using the PCR-RFLP technique. The specimens were genotyped by agarose gel electrophoresis. Finally, the data were analyzed in SPSS software (version 26). (Ethic code: Ir.arakmu.rec.1396.25) Findings: There was a statistically significant difference between the two groups of patients and controls regarding three genotypes of the rs1801133 locus (P=0.006). Moreover, the CT genotype (P=0.001, OR=2.471, 95% CI=1.229-4.965) and total TT and CC genotypes (P=0.000, OR=3.375, 95% CI=1.716-6.637) were significantly associated with the risk of breast cancer. However, the TT genotype (P=0.538) showed a protective role against breast cancer. In contrast, there was no statistically significant difference between the two groups of patients and controls in terms of three genotypes of the rs1801131 locus (P=0.149), which is consistent with the results of some studies. Discussion & Conclusion: The rs1801133 polymorphism of the MTHFR gene can be used as a marker in clinical prognostic studies related to the risk of breast cancer.http://sjimu.medilam.ac.ir/article-1-7403-en.htmlbreast cancermthfrpcr-rflppolymorphismrs1801131rs1801133 |
| spellingShingle | Ahmad Hamta Zahra Bozorgi Moghadam The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām breast cancer mthfr pcr-rflp polymorphism rs1801131 rs1801133 |
| title | The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility |
| title_full | The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility |
| title_fullStr | The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility |
| title_full_unstemmed | The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility |
| title_short | The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility |
| title_sort | association of the mthfr gene polymorphisms with breast cancer susceptibility |
| topic | breast cancer mthfr pcr-rflp polymorphism rs1801131 rs1801133 |
| url | http://sjimu.medilam.ac.ir/article-1-7403-en.html |
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