A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease
Increasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodon...
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MDPI AG
2020-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/5/1405 |
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| author | Alfonso Varela-López Pedro Bullón César L. Ramírez-Tortosa María D. Navarro-Hortal María Robles-Almazán Beatriz Bullón Mario D. Cordero Maurizio Battino José L. Quiles |
| author_facet | Alfonso Varela-López Pedro Bullón César L. Ramírez-Tortosa María D. Navarro-Hortal María Robles-Almazán Beatriz Bullón Mario D. Cordero Maurizio Battino José L. Quiles |
| author_sort | Alfonso Varela-López |
| collection | DOAJ |
| description | Increasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard <i>w</i>/<i>w</i>) and cholesterol (1.3% <i>w</i>/<i>w</i>) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from <i>Porphyromonas gingivalis</i>. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions. |
| first_indexed | 2024-03-10T19:50:31Z |
| format | Article |
| id | doaj.art-551a66c3b51949dba0518175cf9f7920 |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T19:50:31Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-551a66c3b51949dba0518175cf9f79202023-11-20T00:22:52ZengMDPI AGNutrients2072-66432020-05-01125140510.3390/nu12051405A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal DiseaseAlfonso Varela-López0Pedro Bullón1César L. Ramírez-Tortosa2María D. Navarro-Hortal3María Robles-Almazán4Beatriz Bullón5Mario D. Cordero6Maurizio Battino7José L. Quiles8Institute of Nutrition and Food Technology “José Mataix Verdú”, Department of Physiology, Biomedical Research Center, University of Granada, Avda del Conocimiento sn, Armilla, 18016 Granada, SpainDepartment of Stomalogy, Dental School, University of Sevilla, C/Avicena s.n., 41009 Sevilla, SpainUGC de Anatomía Patológica, Hospital San Cecilio de Granada, Avda, Conocimiento s/n, 18100 Granada, SpainInstitute of Nutrition and Food Technology “José Mataix Verdú”, Department of Physiology, Biomedical Research Center, University of Granada, Avda del Conocimiento sn, Armilla, 18016 Granada, SpainPathological Anatomy Department, Jaen Hospital, 23007 Jaen, SpainDepartment of Stomalogy, Dental School, University of Sevilla, C/Avicena s.n., 41009 Sevilla, SpainCátedra de Reproducción y Genética Humana del Instituto para el Estudio de la Biología de la Reproducción Humana (INEBIR), Universidad Europea del Atlántico (UNEATLANTICO)-Fundación Universitaria Iberoamericana (FUNIBER), 39011 Santander, SpainDepartment of Clinical Sicences, Università Politecnica delle Marche, 60131 Ancona, ItalyInstitute of Nutrition and Food Technology “José Mataix Verdú”, Department of Physiology, Biomedical Research Center, University of Granada, Avda del Conocimiento sn, Armilla, 18016 Granada, SpainIncreasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard <i>w</i>/<i>w</i>) and cholesterol (1.3% <i>w</i>/<i>w</i>) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from <i>Porphyromonas gingivalis</i>. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions.https://www.mdpi.com/2072-6643/12/5/1405atherogenicatherosclerosisNASHnon-alcoholic fatty liver diseaseperiodontal diseasesperiodontitis |
| spellingShingle | Alfonso Varela-López Pedro Bullón César L. Ramírez-Tortosa María D. Navarro-Hortal María Robles-Almazán Beatriz Bullón Mario D. Cordero Maurizio Battino José L. Quiles A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease Nutrients atherogenic atherosclerosis NASH non-alcoholic fatty liver disease periodontal diseases periodontitis |
| title | A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease |
| title_full | A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease |
| title_fullStr | A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease |
| title_full_unstemmed | A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease |
| title_short | A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease |
| title_sort | diet rich in saturated fat and cholesterol aggravates the effect of bacterial lipopolysaccharide on alveolar bone loss in a rabbit model of periodontal disease |
| topic | atherogenic atherosclerosis NASH non-alcoholic fatty liver disease periodontal diseases periodontitis |
| url | https://www.mdpi.com/2072-6643/12/5/1405 |
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