Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis

The investigation was designed to isolate and identify the proteolytic microorganisms inhabiting salted fish. Bacillus subtilis was chosen as the most promising protease producer. Some properties of the crude protease are presented, the effect of metal ions on protease production has been studied. I...

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Main Authors: M. H. Abd-Alla, S. A. Omar, M. A. El-Nagdy
Format: Article
Language:English
Published: Polish Botanical Society 2014-01-01
Series:Acta Societatis Botanicorum Poloniae
Subjects:
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/1116
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author M. H. Abd-Alla
S. A. Omar
M. A. El-Nagdy
author_facet M. H. Abd-Alla
S. A. Omar
M. A. El-Nagdy
author_sort M. H. Abd-Alla
collection DOAJ
description The investigation was designed to isolate and identify the proteolytic microorganisms inhabiting salted fish. Bacillus subtilis was chosen as the most promising protease producer. Some properties of the crude protease are presented, the effect of metal ions on protease production has been studied. It was shown that Ca2+ and Mg2+ stimulated, while Co2+ , Zn2+ and Cu2+ inhibited the enzyme production. The effect of temperature and pH and salt tolerance have also been studied. Protease activity was stable in 25% NaCl. The favourable characteristics of the enzyme might have extensive application in laundry detergents and in tanning industry.
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spelling doaj.art-551a77fe126040b3ae6b5bd9147a55f32022-12-22T02:36:13ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01633-430330710.5586/asbp.1994.042852Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilisM. H. Abd-Alla0S. A. Omar1M. A. El-Nagdy2Assiut UniversityAssiut UniversityAssiut UniversityThe investigation was designed to isolate and identify the proteolytic microorganisms inhabiting salted fish. Bacillus subtilis was chosen as the most promising protease producer. Some properties of the crude protease are presented, the effect of metal ions on protease production has been studied. It was shown that Ca2+ and Mg2+ stimulated, while Co2+ , Zn2+ and Cu2+ inhibited the enzyme production. The effect of temperature and pH and salt tolerance have also been studied. Protease activity was stable in 25% NaCl. The favourable characteristics of the enzyme might have extensive application in laundry detergents and in tanning industry.https://pbsociety.org.pl/journals/index.php/asbp/article/view/1116bacteriafungiextracellular proteasesBacillus subtilisproteolytic activity
spellingShingle M. H. Abd-Alla
S. A. Omar
M. A. El-Nagdy
Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
Acta Societatis Botanicorum Poloniae
bacteria
fungi
extracellular proteases
Bacillus subtilis
proteolytic activity
title Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
title_full Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
title_fullStr Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
title_full_unstemmed Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
title_short Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
title_sort protease producing microorganisms inhabiting salted fish moloha with special reference to protease activity of bacillus subtilis
topic bacteria
fungi
extracellular proteases
Bacillus subtilis
proteolytic activity
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/1116
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AT saomar proteaseproducingmicroorganismsinhabitingsaltedfishmolohawithspecialreferencetoproteaseactivityofbacillussubtilis
AT maelnagdy proteaseproducingmicroorganismsinhabitingsaltedfishmolohawithspecialreferencetoproteaseactivityofbacillussubtilis