Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis
The investigation was designed to isolate and identify the proteolytic microorganisms inhabiting salted fish. Bacillus subtilis was chosen as the most promising protease producer. Some properties of the crude protease are presented, the effect of metal ions on protease production has been studied. I...
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Format: | Article |
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Polish Botanical Society
2014-01-01
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Series: | Acta Societatis Botanicorum Poloniae |
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Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1116 |
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author | M. H. Abd-Alla S. A. Omar M. A. El-Nagdy |
author_facet | M. H. Abd-Alla S. A. Omar M. A. El-Nagdy |
author_sort | M. H. Abd-Alla |
collection | DOAJ |
description | The investigation was designed to isolate and identify the proteolytic microorganisms inhabiting salted fish. Bacillus subtilis was chosen as the most promising protease producer. Some properties of the crude protease are presented, the effect of metal ions on protease production has been studied. It was shown that Ca2+ and Mg2+ stimulated, while Co2+ , Zn2+ and Cu2+ inhibited the enzyme production. The effect of temperature and pH and salt tolerance have also been studied. Protease activity was stable in 25% NaCl. The favourable characteristics of the enzyme might have extensive application in laundry detergents and in tanning industry. |
first_indexed | 2024-04-13T18:01:37Z |
format | Article |
id | doaj.art-551a77fe126040b3ae6b5bd9147a55f3 |
institution | Directory Open Access Journal |
issn | 2083-9480 |
language | English |
last_indexed | 2024-04-13T18:01:37Z |
publishDate | 2014-01-01 |
publisher | Polish Botanical Society |
record_format | Article |
series | Acta Societatis Botanicorum Poloniae |
spelling | doaj.art-551a77fe126040b3ae6b5bd9147a55f32022-12-22T02:36:13ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01633-430330710.5586/asbp.1994.042852Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilisM. H. Abd-Alla0S. A. Omar1M. A. El-Nagdy2Assiut UniversityAssiut UniversityAssiut UniversityThe investigation was designed to isolate and identify the proteolytic microorganisms inhabiting salted fish. Bacillus subtilis was chosen as the most promising protease producer. Some properties of the crude protease are presented, the effect of metal ions on protease production has been studied. It was shown that Ca2+ and Mg2+ stimulated, while Co2+ , Zn2+ and Cu2+ inhibited the enzyme production. The effect of temperature and pH and salt tolerance have also been studied. Protease activity was stable in 25% NaCl. The favourable characteristics of the enzyme might have extensive application in laundry detergents and in tanning industry.https://pbsociety.org.pl/journals/index.php/asbp/article/view/1116bacteriafungiextracellular proteasesBacillus subtilisproteolytic activity |
spellingShingle | M. H. Abd-Alla S. A. Omar M. A. El-Nagdy Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis Acta Societatis Botanicorum Poloniae bacteria fungi extracellular proteases Bacillus subtilis proteolytic activity |
title | Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis |
title_full | Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis |
title_fullStr | Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis |
title_full_unstemmed | Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis |
title_short | Protease-producing microorganisms inhabiting salted fish (Moloha) with special reference to protease activity of Bacillus subtilis |
title_sort | protease producing microorganisms inhabiting salted fish moloha with special reference to protease activity of bacillus subtilis |
topic | bacteria fungi extracellular proteases Bacillus subtilis proteolytic activity |
url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1116 |
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