DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae
The euryhaline copepod Pseudodiaptomus inopinus play important roles in coastal waters as vectors of docosahexanoic (DHA) and eicosapentaenoic (EPA) acids for larval fish. While DHA and EPA in polar lipids (PLs) are more effective for fish larval development than non-polar lipid forms (NLs), there i...
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| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.632876/full |
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| author | Hideaki Matsui Taku Sasaki Toru Kobari Toru Kobari Viliame Waqalevu Kazuma Kikuchi Manabu Ishikawa Manabu Ishikawa Tomonari Kotani Tomonari Kotani |
| author_facet | Hideaki Matsui Taku Sasaki Toru Kobari Toru Kobari Viliame Waqalevu Kazuma Kikuchi Manabu Ishikawa Manabu Ishikawa Tomonari Kotani Tomonari Kotani |
| author_sort | Hideaki Matsui |
| collection | DOAJ |
| description | The euryhaline copepod Pseudodiaptomus inopinus play important roles in coastal waters as vectors of docosahexanoic (DHA) and eicosapentaenoic (EPA) acids for larval fish. While DHA and EPA in polar lipids (PLs) are more effective for fish larval development than non-polar lipid forms (NLs), there is little knowledge how much these lipids are accumulated in copepods from microalgae and are effective for early development of fish larvae. We report PLs fatty acid profiles of P. inopinus fed DHA-poor microalgae and evaluate its significance as a food source for larvae development of Pagrus major, compared with DHA-enriched rotifers. Copepods and rotifers were fed a mixed diet of three algal species (Phaeodactylum tricornutum, Tisochrysis lutea, and Pavlova lutheri), in addition of DHA-supplemented Super Fresh Chlorella (SFC) for rotifers. Compared with SFC, the algal mixture had higher EPA but lower DHA. Copepods had higher DHA and EPA in total lipids than rotifers fed each diet. Copepod PLs were specifically enriched with DHA and their contents were higher than both rotifers. On the other hand, PLs EPA contents were comparable between preys, indicating that copepods selectively fortified the PLs. Fish culture experiment showed that larvae fed copepods had higher growth than those fed SFC-enriched rotifers. Principal component analysis for each organism fatty acid composition emphasized trophic modification of DHA by copepods toward larval fish. This study highlighted that P. inopinus contribute to enhanced growth of coastal larval fish by efficiently transferring DHA via copepod fatty acid metabolism. |
| first_indexed | 2024-12-14T12:10:45Z |
| format | Article |
| id | doaj.art-551ad2ee427f486ba6420b4ff55e39ce |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-14T12:10:45Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-551ad2ee427f486ba6420b4ff55e39ce2022-12-21T23:01:44ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-02-01810.3389/fmars.2021.632876632876DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major LarvaeHideaki Matsui0Taku Sasaki1Toru Kobari2Toru Kobari3Viliame Waqalevu4Kazuma Kikuchi5Manabu Ishikawa6Manabu Ishikawa7Tomonari Kotani8Tomonari Kotani9Faculty of Fisheries, Kagoshima University, Kagoshima, JapanGraduate School of Fisheries, Kagoshima University, Kagoshima, JapanFaculty of Fisheries, Kagoshima University, Kagoshima, JapanGraduate School of Fisheries, Kagoshima University, Kagoshima, JapanEPHE-UPVD-CNRS, USR 3278 CRIOBE BP 1013, PSL Research University, Moorea, French PolynesiaGraduate School of Fisheries, Kagoshima University, Kagoshima, JapanFaculty of Fisheries, Kagoshima University, Kagoshima, JapanGraduate School of Fisheries, Kagoshima University, Kagoshima, JapanFaculty of Fisheries, Kagoshima University, Kagoshima, JapanGraduate School of Fisheries, Kagoshima University, Kagoshima, JapanThe euryhaline copepod Pseudodiaptomus inopinus play important roles in coastal waters as vectors of docosahexanoic (DHA) and eicosapentaenoic (EPA) acids for larval fish. While DHA and EPA in polar lipids (PLs) are more effective for fish larval development than non-polar lipid forms (NLs), there is little knowledge how much these lipids are accumulated in copepods from microalgae and are effective for early development of fish larvae. We report PLs fatty acid profiles of P. inopinus fed DHA-poor microalgae and evaluate its significance as a food source for larvae development of Pagrus major, compared with DHA-enriched rotifers. Copepods and rotifers were fed a mixed diet of three algal species (Phaeodactylum tricornutum, Tisochrysis lutea, and Pavlova lutheri), in addition of DHA-supplemented Super Fresh Chlorella (SFC) for rotifers. Compared with SFC, the algal mixture had higher EPA but lower DHA. Copepods had higher DHA and EPA in total lipids than rotifers fed each diet. Copepod PLs were specifically enriched with DHA and their contents were higher than both rotifers. On the other hand, PLs EPA contents were comparable between preys, indicating that copepods selectively fortified the PLs. Fish culture experiment showed that larvae fed copepods had higher growth than those fed SFC-enriched rotifers. Principal component analysis for each organism fatty acid composition emphasized trophic modification of DHA by copepods toward larval fish. This study highlighted that P. inopinus contribute to enhanced growth of coastal larval fish by efficiently transferring DHA via copepod fatty acid metabolism.https://www.frontiersin.org/articles/10.3389/fmars.2021.632876/fullcoastal waterscopepodDHAfish larvaelipidsmicroalgae |
| spellingShingle | Hideaki Matsui Taku Sasaki Toru Kobari Toru Kobari Viliame Waqalevu Kazuma Kikuchi Manabu Ishikawa Manabu Ishikawa Tomonari Kotani Tomonari Kotani DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae Frontiers in Marine Science coastal waters copepod DHA fish larvae lipids microalgae |
| title | DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae |
| title_full | DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae |
| title_fullStr | DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae |
| title_full_unstemmed | DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae |
| title_short | DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae |
| title_sort | dha accumulation in the polar lipids of the euryhaline copepod pseudodiaptomus inopinus and its transfer to red sea bream pagrus major larvae |
| topic | coastal waters copepod DHA fish larvae lipids microalgae |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.632876/full |
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