Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
This work aimed to evaluate the antioxidant capacities and cytotoxic attributes of Alternanthera sissoo (AS) and Alternanthera bettzickiana Green (AB) which are commonly grown in Thailand, yet not extensively-studied. Dried leaves were extracted by maceration using ethanol for 48 h and ethanolic ex...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
AcademicPres
2022-09-01
|
Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
Subjects: | |
Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12776 |
_version_ | 1811203199291359232 |
---|---|
author | Worachot SAENGHA Thipphiya KARIRAT Benjaporn BURANRAT Teeraporn KATISART Nyuk Ling MA Vijitra LUANG-IN |
author_facet | Worachot SAENGHA Thipphiya KARIRAT Benjaporn BURANRAT Teeraporn KATISART Nyuk Ling MA Vijitra LUANG-IN |
author_sort | Worachot SAENGHA |
collection | DOAJ |
description |
This work aimed to evaluate the antioxidant capacities and cytotoxic attributes of Alternanthera sissoo (AS) and Alternanthera bettzickiana Green (AB) which are commonly grown in Thailand, yet not extensively-studied. Dried leaves were extracted by maceration using ethanol for 48 h and ethanolic extract solution was used. Cytotoxic, anti-proliferative, anti-migratory capacities and apoptosis-related gene expressions of AS and AB extracts on MCF-7, HepG2, and HeLa cell lines were investigated using 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetra zolium bromide (MTT) assay, anti-colony formation and wound healing test, respectively. The antioxidant activities by DPPH assay and FRAP assay of AS (130.62±1.61 µg TE/g extract and 24.76 ± 0.54 mg Fe(II)/g extract, respectively) were significantly higher than those of AB (104.34±3.68 µg TE/g extract and 23.13± 0.90 mg Fe(II)/g extract, respectively). Likewise, total phenolic content and the total flavonoid content of AS (7.43±0.39 mg GAE/g and 184.22±3.20 mg RE/g extract, respectively) were significantly higher than AB (6.10±0.29 mg GAE/g extract and 168.07±7.90 mg RE/g extract, respectively). HPLC analysis showed the predominance of myricetin, rutin and ferulic acid. For MCF-7, HepG2 and HeLa, the lowest IC50 values of 48.53 µg/mL, 69.94 µg/mL and 43.98 µg/mL, respectively were found in AS extract at 72 h exposure using MTT assay. Apoptotic bodies, gene expression and protein expressions related to apoptosis were more pronounced in AS treatment. Both AS and AB can be served as local foods with chemopreventive effects against breast, liver and cervical cancers. This work provided a foundation for future pharmacological research on Alternanthera plant extracts.
|
first_indexed | 2024-04-12T02:51:50Z |
format | Article |
id | doaj.art-551d4a837eb34f44be122fcaceef9c2c |
institution | Directory Open Access Journal |
issn | 0255-965X 1842-4309 |
language | English |
last_indexed | 2024-04-12T02:51:50Z |
publishDate | 2022-09-01 |
publisher | AcademicPres |
record_format | Article |
series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
spelling | doaj.art-551d4a837eb34f44be122fcaceef9c2c2022-12-22T03:50:59ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092022-09-0150310.15835/nbha50312776Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cellsWorachot SAENGHA0Thipphiya KARIRAT1Benjaporn BURANRAT2Teeraporn KATISART3Nyuk Ling MA4Vijitra LUANG-IN5Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Medicine, Mahasarakham 44000Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150University Malaysia Terengganu, Faculty of Science and Marine Environment, 21030, Kuala Nerus, TerengganuMahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150 This work aimed to evaluate the antioxidant capacities and cytotoxic attributes of Alternanthera sissoo (AS) and Alternanthera bettzickiana Green (AB) which are commonly grown in Thailand, yet not extensively-studied. Dried leaves were extracted by maceration using ethanol for 48 h and ethanolic extract solution was used. Cytotoxic, anti-proliferative, anti-migratory capacities and apoptosis-related gene expressions of AS and AB extracts on MCF-7, HepG2, and HeLa cell lines were investigated using 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetra zolium bromide (MTT) assay, anti-colony formation and wound healing test, respectively. The antioxidant activities by DPPH assay and FRAP assay of AS (130.62±1.61 µg TE/g extract and 24.76 ± 0.54 mg Fe(II)/g extract, respectively) were significantly higher than those of AB (104.34±3.68 µg TE/g extract and 23.13± 0.90 mg Fe(II)/g extract, respectively). Likewise, total phenolic content and the total flavonoid content of AS (7.43±0.39 mg GAE/g and 184.22±3.20 mg RE/g extract, respectively) were significantly higher than AB (6.10±0.29 mg GAE/g extract and 168.07±7.90 mg RE/g extract, respectively). HPLC analysis showed the predominance of myricetin, rutin and ferulic acid. For MCF-7, HepG2 and HeLa, the lowest IC50 values of 48.53 µg/mL, 69.94 µg/mL and 43.98 µg/mL, respectively were found in AS extract at 72 h exposure using MTT assay. Apoptotic bodies, gene expression and protein expressions related to apoptosis were more pronounced in AS treatment. Both AS and AB can be served as local foods with chemopreventive effects against breast, liver and cervical cancers. This work provided a foundation for future pharmacological research on Alternanthera plant extracts. https://www.notulaebotanicae.ro/index.php/nbha/article/view/12776A549Brazilian spinachflavonoidHepG2 joyweedMCF-7 |
spellingShingle | Worachot SAENGHA Thipphiya KARIRAT Benjaporn BURANRAT Teeraporn KATISART Nyuk Ling MA Vijitra LUANG-IN Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells Notulae Botanicae Horti Agrobotanici Cluj-Napoca A549 Brazilian spinach flavonoid HepG2 joyweed MCF-7 |
title | Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells |
title_full | Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells |
title_fullStr | Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells |
title_full_unstemmed | Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells |
title_short | Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells |
title_sort | antioxidant properties and cytotoxic effects of alternanthera sissoo and alternanthera bettzickiana extracts against cancer cells |
topic | A549 Brazilian spinach flavonoid HepG2 joyweed MCF-7 |
url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12776 |
work_keys_str_mv | AT worachotsaengha antioxidantpropertiesandcytotoxiceffectsofalternantherasissooandalternantherabettzickianaextractsagainstcancercells AT thipphiyakarirat antioxidantpropertiesandcytotoxiceffectsofalternantherasissooandalternantherabettzickianaextractsagainstcancercells AT benjapornburanrat antioxidantpropertiesandcytotoxiceffectsofalternantherasissooandalternantherabettzickianaextractsagainstcancercells AT teerapornkatisart antioxidantpropertiesandcytotoxiceffectsofalternantherasissooandalternantherabettzickianaextractsagainstcancercells AT nyuklingma antioxidantpropertiesandcytotoxiceffectsofalternantherasissooandalternantherabettzickianaextractsagainstcancercells AT vijitraluangin antioxidantpropertiesandcytotoxiceffectsofalternantherasissooandalternantherabettzickianaextractsagainstcancercells |