Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells

This work aimed to evaluate the antioxidant capacities and cytotoxic attributes of Alternanthera sissoo (AS) and Alternanthera bettzickiana Green (AB) which are commonly grown in Thailand, yet not extensively-studied. Dried leaves were extracted by maceration using ethanol for 48 h and ethanolic ex...

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Main Authors: Worachot SAENGHA, Thipphiya KARIRAT, Benjaporn BURANRAT, Teeraporn KATISART, Nyuk Ling MA, Vijitra LUANG-IN
Format: Article
Language:English
Published: AcademicPres 2022-09-01
Series:Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Subjects:
Online Access:https://www.notulaebotanicae.ro/index.php/nbha/article/view/12776
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author Worachot SAENGHA
Thipphiya KARIRAT
Benjaporn BURANRAT
Teeraporn KATISART
Nyuk Ling MA
Vijitra LUANG-IN
author_facet Worachot SAENGHA
Thipphiya KARIRAT
Benjaporn BURANRAT
Teeraporn KATISART
Nyuk Ling MA
Vijitra LUANG-IN
author_sort Worachot SAENGHA
collection DOAJ
description This work aimed to evaluate the antioxidant capacities and cytotoxic attributes of Alternanthera sissoo (AS) and Alternanthera bettzickiana Green (AB) which are commonly grown in Thailand, yet not extensively-studied. Dried leaves were extracted by maceration using ethanol for 48 h and ethanolic extract solution was used. Cytotoxic, anti-proliferative, anti-migratory capacities and apoptosis-related gene expressions of AS and AB extracts on MCF-7, HepG2, and HeLa cell lines were investigated using 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetra zolium bromide (MTT) assay, anti-colony formation and wound healing test, respectively. The antioxidant activities by DPPH assay and FRAP assay of AS (130.62±1.61 µg TE/g extract and 24.76 ± 0.54 mg Fe(II)/g extract, respectively) were significantly higher than those of AB (104.34±3.68 µg TE/g extract and 23.13± 0.90 mg Fe(II)/g extract, respectively). Likewise, total phenolic content and the total flavonoid content of AS (7.43±0.39 mg GAE/g and 184.22±3.20 mg RE/g extract, respectively) were significantly higher than AB (6.10±0.29 mg GAE/g extract and 168.07±7.90 mg RE/g extract, respectively). HPLC analysis showed the predominance of myricetin, rutin and ferulic acid. For MCF-7, HepG2 and HeLa, the lowest IC50 values of 48.53 µg/mL, 69.94 µg/mL and 43.98 µg/mL, respectively were found in AS extract at 72 h exposure using MTT assay. Apoptotic bodies, gene expression and protein expressions related to apoptosis were more pronounced in AS treatment. Both AS and AB can be served as local foods with chemopreventive effects against breast, liver and cervical cancers. This work provided a foundation for future pharmacological research on Alternanthera plant extracts.
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spelling doaj.art-551d4a837eb34f44be122fcaceef9c2c2022-12-22T03:50:59ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092022-09-0150310.15835/nbha50312776Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cellsWorachot SAENGHA0Thipphiya KARIRAT1Benjaporn BURANRAT2Teeraporn KATISART3Nyuk Ling MA4Vijitra LUANG-IN5Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Medicine, Mahasarakham 44000Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150University Malaysia Terengganu, Faculty of Science and Marine Environment, 21030, Kuala Nerus, TerengganuMahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150 This work aimed to evaluate the antioxidant capacities and cytotoxic attributes of Alternanthera sissoo (AS) and Alternanthera bettzickiana Green (AB) which are commonly grown in Thailand, yet not extensively-studied. Dried leaves were extracted by maceration using ethanol for 48 h and ethanolic extract solution was used. Cytotoxic, anti-proliferative, anti-migratory capacities and apoptosis-related gene expressions of AS and AB extracts on MCF-7, HepG2, and HeLa cell lines were investigated using 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetra zolium bromide (MTT) assay, anti-colony formation and wound healing test, respectively. The antioxidant activities by DPPH assay and FRAP assay of AS (130.62±1.61 µg TE/g extract and 24.76 ± 0.54 mg Fe(II)/g extract, respectively) were significantly higher than those of AB (104.34±3.68 µg TE/g extract and 23.13± 0.90 mg Fe(II)/g extract, respectively). Likewise, total phenolic content and the total flavonoid content of AS (7.43±0.39 mg GAE/g and 184.22±3.20 mg RE/g extract, respectively) were significantly higher than AB (6.10±0.29 mg GAE/g extract and 168.07±7.90 mg RE/g extract, respectively). HPLC analysis showed the predominance of myricetin, rutin and ferulic acid. For MCF-7, HepG2 and HeLa, the lowest IC50 values of 48.53 µg/mL, 69.94 µg/mL and 43.98 µg/mL, respectively were found in AS extract at 72 h exposure using MTT assay. Apoptotic bodies, gene expression and protein expressions related to apoptosis were more pronounced in AS treatment. Both AS and AB can be served as local foods with chemopreventive effects against breast, liver and cervical cancers. This work provided a foundation for future pharmacological research on Alternanthera plant extracts. https://www.notulaebotanicae.ro/index.php/nbha/article/view/12776A549Brazilian spinachflavonoidHepG2 joyweedMCF-7
spellingShingle Worachot SAENGHA
Thipphiya KARIRAT
Benjaporn BURANRAT
Teeraporn KATISART
Nyuk Ling MA
Vijitra LUANG-IN
Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
Notulae Botanicae Horti Agrobotanici Cluj-Napoca
A549
Brazilian spinach
flavonoid
HepG2
joyweed
MCF-7
title Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
title_full Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
title_fullStr Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
title_full_unstemmed Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
title_short Antioxidant properties and cytotoxic effects of Alternanthera sissoo and Alternanthera bettzickiana extracts against cancer cells
title_sort antioxidant properties and cytotoxic effects of alternanthera sissoo and alternanthera bettzickiana extracts against cancer cells
topic A549
Brazilian spinach
flavonoid
HepG2
joyweed
MCF-7
url https://www.notulaebotanicae.ro/index.php/nbha/article/view/12776
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