Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
IntroductionEnvironmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic st...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1200926/full |
| _version_ | 1797810986789371904 |
|---|---|
| author | Vidhya Prakash Aravind Madhavan Archana Palillam Veedu Pradeesh Babu Abhirami Jothish Sruthy S. Nair Alin Suhail Meera Prabhakar Thasleema Sain Raveena Rajan Priyanka Somanathan Kuniyil Abhinand Bipin G. Nair Sanjay Pal |
| author_facet | Vidhya Prakash Aravind Madhavan Archana Palillam Veedu Pradeesh Babu Abhirami Jothish Sruthy S. Nair Alin Suhail Meera Prabhakar Thasleema Sain Raveena Rajan Priyanka Somanathan Kuniyil Abhinand Bipin G. Nair Sanjay Pal |
| author_sort | Vidhya Prakash |
| collection | DOAJ |
| description | IntroductionEnvironmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic strains from fermented foods can be an effective strategy to inhibit enteric pathogens and prevent chronic gut inflammation.MethodsWe isolated potential strains from fermented rice water and lemon pickle and investigated their cell surface properties, antagonistic properties, adhesion to HT-29 cells, and inhibition of pathogen adherence to HT-29 cells. Bacteriocin-like inhibitory substances (BLIS) were purified, and in vivo, survival studies in Caenorhabditis elegans infected with Salmonella enterica MW116733 were performed. We further checked the expression pattern of pro and anti-inflammatory cytokines (IL-6, IL8, and IL-10) in HT-29 cells supplemented with strains.ResultsThe strains isolated from rice water (RS) and lemon pickle (T1) were identified as Limosilactobacillus fermentum MN410703 and MN410702, respectively. Strains showed probiotic properties like tolerance to low pH (pH 3.0), bile salts up to 0.5%, simulated gastric juice at low pH, and binding to extracellular matrix molecules. Auto-aggregation of T1 was in the range of 85% and significantly co-aggregated with Klebsiella pneumoniae, S. enterica, and Escherichia coli at 48, 79, and 65%, respectively. Both strains had a higher binding affinity to gelatin and heparin compared to Bacillus clausii. Susceptibility to most aminoglycoside, cephalosporin, and macrolide classes of antibiotics was also observed. RS showed BLIS activity against K. pneumoniae, S. aureus, and S. enterica at 60, 48, and 30%, respectively, and the protective effects of BLIS from RS in the C. elegans infection model demonstrated a 70% survival rate of the worms infected with S. enterica. RS and T1 demonstrated binding efficiency to HT-29 cell lines in the 38–46% range, and both strains inhibited the adhesion of E. coli MDR and S. enterica. Upregulation of IL-6 and IL-10 and the downregulation of IL-8 were observed when HT-29 cells were treated with RS, indicating the immunomodulatory effects of the strain.DiscussionThe potential strains identified could effectively inhibit enteric pathogens and prevent environmental enteropathy. |
| first_indexed | 2024-03-13T07:16:58Z |
| format | Article |
| id | doaj.art-551db72854fe4ab188be4aae985e9b2d |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-13T07:16:58Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-551db72854fe4ab188be4aae985e9b2d2023-06-05T05:04:39ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-06-011010.3389/fnut.2023.12009261200926Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathyVidhya PrakashAravind MadhavanArchana Palillam VeeduPradeesh BabuAbhirami JothishSruthy S. NairAlin SuhailMeera PrabhakarThasleema SainRaveena RajanPriyanka SomanathanKuniyil AbhinandBipin G. NairSanjay PalIntroductionEnvironmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic strains from fermented foods can be an effective strategy to inhibit enteric pathogens and prevent chronic gut inflammation.MethodsWe isolated potential strains from fermented rice water and lemon pickle and investigated their cell surface properties, antagonistic properties, adhesion to HT-29 cells, and inhibition of pathogen adherence to HT-29 cells. Bacteriocin-like inhibitory substances (BLIS) were purified, and in vivo, survival studies in Caenorhabditis elegans infected with Salmonella enterica MW116733 were performed. We further checked the expression pattern of pro and anti-inflammatory cytokines (IL-6, IL8, and IL-10) in HT-29 cells supplemented with strains.ResultsThe strains isolated from rice water (RS) and lemon pickle (T1) were identified as Limosilactobacillus fermentum MN410703 and MN410702, respectively. Strains showed probiotic properties like tolerance to low pH (pH 3.0), bile salts up to 0.5%, simulated gastric juice at low pH, and binding to extracellular matrix molecules. Auto-aggregation of T1 was in the range of 85% and significantly co-aggregated with Klebsiella pneumoniae, S. enterica, and Escherichia coli at 48, 79, and 65%, respectively. Both strains had a higher binding affinity to gelatin and heparin compared to Bacillus clausii. Susceptibility to most aminoglycoside, cephalosporin, and macrolide classes of antibiotics was also observed. RS showed BLIS activity against K. pneumoniae, S. aureus, and S. enterica at 60, 48, and 30%, respectively, and the protective effects of BLIS from RS in the C. elegans infection model demonstrated a 70% survival rate of the worms infected with S. enterica. RS and T1 demonstrated binding efficiency to HT-29 cell lines in the 38–46% range, and both strains inhibited the adhesion of E. coli MDR and S. enterica. Upregulation of IL-6 and IL-10 and the downregulation of IL-8 were observed when HT-29 cells were treated with RS, indicating the immunomodulatory effects of the strain.DiscussionThe potential strains identified could effectively inhibit enteric pathogens and prevent environmental enteropathy.https://www.frontiersin.org/articles/10.3389/fnut.2023.1200926/fullprobioticsenvironmental enteropathyCaenorhabditis elegansfermented riceLimosilactobacillus fermentumHT 29 |
| spellingShingle | Vidhya Prakash Aravind Madhavan Archana Palillam Veedu Pradeesh Babu Abhirami Jothish Sruthy S. Nair Alin Suhail Meera Prabhakar Thasleema Sain Raveena Rajan Priyanka Somanathan Kuniyil Abhinand Bipin G. Nair Sanjay Pal Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy Frontiers in Nutrition probiotics environmental enteropathy Caenorhabditis elegans fermented rice Limosilactobacillus fermentum HT 29 |
| title | Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy |
| title_full | Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy |
| title_fullStr | Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy |
| title_full_unstemmed | Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy |
| title_short | Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy |
| title_sort | harnessing the probiotic properties and immunomodulatory effects of fermented food derived limosilactobacillus fermentum strains implications for environmental enteropathy |
| topic | probiotics environmental enteropathy Caenorhabditis elegans fermented rice Limosilactobacillus fermentum HT 29 |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1200926/full |
| work_keys_str_mv | AT vidhyaprakash harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT aravindmadhavan harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT archanapalillamveedu harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT pradeeshbabu harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT abhiramijothish harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT sruthysnair harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT alinsuhail harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT meeraprabhakar harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT thasleemasain harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT raveenarajan harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT priyankasomanathan harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT kuniyilabhinand harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT bipingnair harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy AT sanjaypal harnessingtheprobioticpropertiesandimmunomodulatoryeffectsoffermentedfoodderivedlimosilactobacillusfermentumstrainsimplicationsforenvironmentalenteropathy |