Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model
Vertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry prote...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-03-01
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| Series: | Pathogens |
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| Online Access: | http://www.mdpi.com/2076-0817/2/2/193 |
| _version_ | 1811298415426928640 |
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| author | Denis Grandgirard Stephen L. Leib Andrew Hemphill Thierry Monney |
| author_facet | Denis Grandgirard Stephen L. Leib Andrew Hemphill Thierry Monney |
| author_sort | Denis Grandgirard |
| collection | DOAJ |
| description | Vertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry protein NcROP2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant BALB/c mice. Protection was associated with a mixed Th1/Th2-type cytokine response. However, the same vaccine formulation elicited a Th2-type immune response in pregnant mice and did not prevent vertical transmission or disease, neither in dams nor in offspring mice. In this study, an alternative vaccine formulation containing recNcMIC3-1-R emulsified in Freund’s incomplete adjuvant, a stimulator of the cellular immunity, was investigated. No protection against vertical transmission and cerebral infection in the pregnant mice and a very limited protective effect in the non-pregnant mice were observed. The vaccine induced a Th1-type immune response characterized by high IgG2a titres and strong IFN-γ expression, which appeared detrimental to pregnancy. |
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| format | Article |
| id | doaj.art-551ff08850b848e594fbe36d9daef9dd |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-13T06:19:55Z |
| publishDate | 2013-03-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-551ff08850b848e594fbe36d9daef9dd2022-12-22T02:58:42ZengMDPI AGPathogens2076-08172013-03-012219320810.3390/pathogens2020193Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse ModelDenis GrandgirardStephen L. LeibAndrew HemphillThierry MonneyVertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry protein NcROP2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant BALB/c mice. Protection was associated with a mixed Th1/Th2-type cytokine response. However, the same vaccine formulation elicited a Th2-type immune response in pregnant mice and did not prevent vertical transmission or disease, neither in dams nor in offspring mice. In this study, an alternative vaccine formulation containing recNcMIC3-1-R emulsified in Freund’s incomplete adjuvant, a stimulator of the cellular immunity, was investigated. No protection against vertical transmission and cerebral infection in the pregnant mice and a very limited protective effect in the non-pregnant mice were observed. The vaccine induced a Th1-type immune response characterized by high IgG2a titres and strong IFN-γ expression, which appeared detrimental to pregnancy.http://www.mdpi.com/2076-0817/2/2/193Neospora caninumvaccinationrecombinant antigenpregnancyvertical transmissioncytokinesmouse modelabortion |
| spellingShingle | Denis Grandgirard Stephen L. Leib Andrew Hemphill Thierry Monney Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model Pathogens Neospora caninum vaccination recombinant antigen pregnancy vertical transmission cytokines mouse model abortion |
| title | Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model |
| title_full | Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model |
| title_fullStr | Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model |
| title_full_unstemmed | Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model |
| title_short | Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model |
| title_sort | use of a th1 stimulator adjuvant for vaccination against neospora caninum infection in the pregnant mouse model |
| topic | Neospora caninum vaccination recombinant antigen pregnancy vertical transmission cytokines mouse model abortion |
| url | http://www.mdpi.com/2076-0817/2/2/193 |
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