Nanoparticles Obtained from Zein for Encapsulation of Mesalazine
We encapsulated MSZ in zein nanoparticles (NP-ZN) using a desolvation method followed by drying in a mini spray dryer. These nanoparticles exhibited a size of 266.6 ± 52 nm, IPD of 0.14 ± 1.1 and zeta potential of −36.4 ± 1.5 mV, suggesting colloidal stability. Quantification using HPLC showed a dru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/12/2830 |
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| author | Izabela Borges C. Lima Lina Clara G. A. I. Moreno Ana Victória Peres Ana Cristina Gramoza Santana Adonias Carvalho Mariana H. Chaves Lorena Lima Rayran Walter Sousa Dalton Dittz Hercília M. L. Rolim Lívio César Cunha Nunes |
| author_facet | Izabela Borges C. Lima Lina Clara G. A. I. Moreno Ana Victória Peres Ana Cristina Gramoza Santana Adonias Carvalho Mariana H. Chaves Lorena Lima Rayran Walter Sousa Dalton Dittz Hercília M. L. Rolim Lívio César Cunha Nunes |
| author_sort | Izabela Borges C. Lima |
| collection | DOAJ |
| description | We encapsulated MSZ in zein nanoparticles (NP-ZN) using a desolvation method followed by drying in a mini spray dryer. These nanoparticles exhibited a size of 266.6 ± 52 nm, IPD of 0.14 ± 1.1 and zeta potential of −36.4 ± 1.5 mV, suggesting colloidal stability. Quantification using HPLC showed a drug-loaded of 43.8 µg/mg. SEM demonstrated a spherical morphology with a size variation from 220 to 400 nm. A FTIR analysis did not show drug spectra in the NPs in relation to the physical mixture, which suggests drug encapsulation without changing its chemical structure. A TGA analysis showed thermal stability up to 300 °C. In vitro release studies demonstrated gastroresistance and a sustained drug release at pH 7.4 (97.67 ± 0.32%) in 120 h. The kinetic model used for the release of MSZ from the NP-ZN in a pH 1.2 medium was the Fickian diffusion, in a pH 6.8 medium it was the Peppas–Sahlin model with the polymeric relaxation mechanism and in a pH 7.4 medium it was the Korsmeyer–Peppas model with the Fickian release mechanism, or “Case I”. An in vitro cytotoxicity study in the CT26.WT cell line showed no basal cytotoxicity up to 500 μg/mL. The NP-ZN showed to be a promising vector for the sustained release of MSZ in the colon by oral route. |
| first_indexed | 2024-03-09T15:57:20Z |
| format | Article |
| id | doaj.art-5520f8d93f2844179b5a9a162940a0ba |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:57:20Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-5520f8d93f2844179b5a9a162940a0ba2023-11-24T17:22:45ZengMDPI AGPharmaceutics1999-49232022-12-011412283010.3390/pharmaceutics14122830Nanoparticles Obtained from Zein for Encapsulation of MesalazineIzabela Borges C. Lima0Lina Clara G. A. I. Moreno1Ana Victória Peres2Ana Cristina Gramoza Santana3Adonias Carvalho4Mariana H. Chaves5Lorena Lima6Rayran Walter Sousa7Dalton Dittz8Hercília M. L. Rolim9Lívio César Cunha Nunes10Laboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilPharmaceutical Nanosystems Laboratory (NANOSFAR), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilLaboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilLaboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilNatural Products Laboratory (LPN), Department of Chemistry, Federal University of Piauí, Teresina 64049-550, PI, BrazilNatural Products Laboratory (LPN), Department of Chemistry, Federal University of Piauí, Teresina 64049-550, PI, BrazilLaboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilLaboratory of Experimental Cancerology (LabCâncer), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilLaboratory of Experimental Cancerology (LabCâncer), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilPharmaceutical Nanosystems Laboratory (NANOSFAR), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilLaboratory of Technological Innovation, Entrepreneurship, Medicines and Related (LITE), Nucleus of Pharmaceutical Technology, Federal University of Piauí, Teresina 64049-550, PI, BrazilWe encapsulated MSZ in zein nanoparticles (NP-ZN) using a desolvation method followed by drying in a mini spray dryer. These nanoparticles exhibited a size of 266.6 ± 52 nm, IPD of 0.14 ± 1.1 and zeta potential of −36.4 ± 1.5 mV, suggesting colloidal stability. Quantification using HPLC showed a drug-loaded of 43.8 µg/mg. SEM demonstrated a spherical morphology with a size variation from 220 to 400 nm. A FTIR analysis did not show drug spectra in the NPs in relation to the physical mixture, which suggests drug encapsulation without changing its chemical structure. A TGA analysis showed thermal stability up to 300 °C. In vitro release studies demonstrated gastroresistance and a sustained drug release at pH 7.4 (97.67 ± 0.32%) in 120 h. The kinetic model used for the release of MSZ from the NP-ZN in a pH 1.2 medium was the Fickian diffusion, in a pH 6.8 medium it was the Peppas–Sahlin model with the polymeric relaxation mechanism and in a pH 7.4 medium it was the Korsmeyer–Peppas model with the Fickian release mechanism, or “Case I”. An in vitro cytotoxicity study in the CT26.WT cell line showed no basal cytotoxicity up to 500 μg/mL. The NP-ZN showed to be a promising vector for the sustained release of MSZ in the colon by oral route.https://www.mdpi.com/1999-4923/14/12/2830natural polymerzein5-ASAnanoparticlescolon |
| spellingShingle | Izabela Borges C. Lima Lina Clara G. A. I. Moreno Ana Victória Peres Ana Cristina Gramoza Santana Adonias Carvalho Mariana H. Chaves Lorena Lima Rayran Walter Sousa Dalton Dittz Hercília M. L. Rolim Lívio César Cunha Nunes Nanoparticles Obtained from Zein for Encapsulation of Mesalazine Pharmaceutics natural polymer zein 5-ASA nanoparticles colon |
| title | Nanoparticles Obtained from Zein for Encapsulation of Mesalazine |
| title_full | Nanoparticles Obtained from Zein for Encapsulation of Mesalazine |
| title_fullStr | Nanoparticles Obtained from Zein for Encapsulation of Mesalazine |
| title_full_unstemmed | Nanoparticles Obtained from Zein for Encapsulation of Mesalazine |
| title_short | Nanoparticles Obtained from Zein for Encapsulation of Mesalazine |
| title_sort | nanoparticles obtained from zein for encapsulation of mesalazine |
| topic | natural polymer zein 5-ASA nanoparticles colon |
| url | https://www.mdpi.com/1999-4923/14/12/2830 |
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