<i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant
<i>Salmonella enterica</i> serovar Enteritidis (<i>S</i>. Enteritidis) is an important zoonotic pathogen that can lead to diarrhea and systemic infections in humans and mortality in animals. This is a major public health issue worldwide. Safe and effective vaccines are urgent...
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MDPI AG
2022-04-01
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author | Xilong Kang Tingting Huang Huanhuan Shen Chuang Meng Xinan Jiao Zhiming Pan |
author_facet | Xilong Kang Tingting Huang Huanhuan Shen Chuang Meng Xinan Jiao Zhiming Pan |
author_sort | Xilong Kang |
collection | DOAJ |
description | <i>Salmonella enterica</i> serovar Enteritidis (<i>S</i>. Enteritidis) is an important zoonotic pathogen that can lead to diarrhea and systemic infections in humans and mortality in animals. This is a major public health issue worldwide. Safe and effective vaccines are urgently needed to control and prevent <i>Salmonella</i> infection. Subunit vaccines are safe and provide targeted protection against <i>Salmonella</i> spp. Here, we developed and evaluated an <i>S</i>. Enteritidis subunit vaccine candidate, the rHis-SseB adjuvant with simvastatin. We amplified the <i>SseB</i> gene from <i>S</i>. Enteritidis C50041 genomic DNA and expressed the recombinant proteins rHis-SseB and rGST-SseB using the <i>Escherichia coli</i> system. Western blotting confirmed the immunoreactivity of recombinant proteins rHis-SseB and rGST-SseB with antisera against <i>Salmonella</i> Enteritidis C50041. In a mouse model of intramuscular vaccination, co-immunization with rHis-SseB and simvastatin significantly enhanced both the SseB-specific antibody titer in serum (humoral immune response) and splenic lymphocyte proliferation (cellular immune response). Co-immunization with rHis-SseB and simvastatin provided 60% protection against subsequent challenge with the <i>S</i>. Enteritidis C50041 strain and decreased bacterial colonization in the liver and spleen. These findings provide a basis for the development of an <i>S</i>. Enteritidis subunit vaccine. |
first_indexed | 2024-03-09T13:10:32Z |
format | Article |
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institution | Directory Open Access Journal |
issn | 2076-0817 |
language | English |
last_indexed | 2024-03-09T13:10:32Z |
publishDate | 2022-04-01 |
publisher | MDPI AG |
record_format | Article |
series | Pathogens |
spelling | doaj.art-55229c6be4324b1cb74d78450beaa6172023-11-30T21:42:18ZengMDPI AGPathogens2076-08172022-04-0111444310.3390/pathogens11040443<i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as AdjuvantXilong Kang0Tingting Huang1Huanhuan Shen2Chuang Meng3Xinan Jiao4Zhiming Pan5Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China<i>Salmonella enterica</i> serovar Enteritidis (<i>S</i>. Enteritidis) is an important zoonotic pathogen that can lead to diarrhea and systemic infections in humans and mortality in animals. This is a major public health issue worldwide. Safe and effective vaccines are urgently needed to control and prevent <i>Salmonella</i> infection. Subunit vaccines are safe and provide targeted protection against <i>Salmonella</i> spp. Here, we developed and evaluated an <i>S</i>. Enteritidis subunit vaccine candidate, the rHis-SseB adjuvant with simvastatin. We amplified the <i>SseB</i> gene from <i>S</i>. Enteritidis C50041 genomic DNA and expressed the recombinant proteins rHis-SseB and rGST-SseB using the <i>Escherichia coli</i> system. Western blotting confirmed the immunoreactivity of recombinant proteins rHis-SseB and rGST-SseB with antisera against <i>Salmonella</i> Enteritidis C50041. In a mouse model of intramuscular vaccination, co-immunization with rHis-SseB and simvastatin significantly enhanced both the SseB-specific antibody titer in serum (humoral immune response) and splenic lymphocyte proliferation (cellular immune response). Co-immunization with rHis-SseB and simvastatin provided 60% protection against subsequent challenge with the <i>S</i>. Enteritidis C50041 strain and decreased bacterial colonization in the liver and spleen. These findings provide a basis for the development of an <i>S</i>. Enteritidis subunit vaccine.https://www.mdpi.com/2076-0817/11/4/443<i>S</i>. Enteritidissubunit vaccineSseBsimvastatinimmune responseimmune protection |
spellingShingle | Xilong Kang Tingting Huang Huanhuan Shen Chuang Meng Xinan Jiao Zhiming Pan <i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant Pathogens <i>S</i>. Enteritidis subunit vaccine SseB simvastatin immune response immune protection |
title | <i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant |
title_full | <i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant |
title_fullStr | <i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant |
title_full_unstemmed | <i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant |
title_short | <i>Salmonella</i> Enteritidis Subunit Vaccine Candidate Based on SseB Protein Co-Delivered with Simvastatin as Adjuvant |
title_sort | i salmonella i enteritidis subunit vaccine candidate based on sseb protein co delivered with simvastatin as adjuvant |
topic | <i>S</i>. Enteritidis subunit vaccine SseB simvastatin immune response immune protection |
url | https://www.mdpi.com/2076-0817/11/4/443 |
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