Immediate reuse of patch-clamp pipettes after ultrasonic cleaning
Abstract The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile. However, the throughput in recordings is limited because of the manual replacement of patch-pipettes after each...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51837-7 |
| _version_ | 1797349992342487040 |
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| author | Kevin Jehasse Jean-Sébastien Jouhanneau Sophie Wetz Alexander Schwedt James F. A. Poulet Peter Neumann-Raizel Björn M. Kampa |
| author_facet | Kevin Jehasse Jean-Sébastien Jouhanneau Sophie Wetz Alexander Schwedt James F. A. Poulet Peter Neumann-Raizel Björn M. Kampa |
| author_sort | Kevin Jehasse |
| collection | DOAJ |
| description | Abstract The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile. However, the throughput in recordings is limited because of the manual replacement of patch-pipettes after each attempt which are often also unsuccessful. This has been overcome by automated cleaning the tips in detergent solutions, allowing to reuse the pipette for further recordings. Here, we developed a novel method of automated cleaning by sonicating the tips within the bath solution wherein the cells are placed, reducing the risk of contaminating the bath solution or internal solution of the recording pipette by any detergent and avoiding the necessity of a separate chamber for cleaning. We showed that the patch-pipettes can be used consecutively at least ten times and that the cleaning process does not negatively impact neither the brain slices nor other patched neurons. This method, combined with automated patch-clamp, highly improves the throughput for single and especially multiple recordings. |
| first_indexed | 2024-03-08T12:38:21Z |
| format | Article |
| id | doaj.art-5525a42883af425ab37f820e962b5190 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:38:21Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5525a42883af425ab37f820e962b51902024-01-21T12:18:34ZengNature PortfolioScientific Reports2045-23222024-01-011411910.1038/s41598-024-51837-7Immediate reuse of patch-clamp pipettes after ultrasonic cleaningKevin Jehasse0Jean-Sébastien Jouhanneau1Sophie Wetz2Alexander Schwedt3James F. A. Poulet4Peter Neumann-Raizel5Björn M. Kampa6Systems Neurophysiology, Institute of Biology II, RWTH-Aachen UniversityMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)Systems Neurophysiology, Institute of Biology II, RWTH-Aachen UniversityCentral Facility for Electron Microscopy, RWTH-Aachen UniversityMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)Luigs & Neumann GmbHSystems Neurophysiology, Institute of Biology II, RWTH-Aachen UniversityAbstract The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile. However, the throughput in recordings is limited because of the manual replacement of patch-pipettes after each attempt which are often also unsuccessful. This has been overcome by automated cleaning the tips in detergent solutions, allowing to reuse the pipette for further recordings. Here, we developed a novel method of automated cleaning by sonicating the tips within the bath solution wherein the cells are placed, reducing the risk of contaminating the bath solution or internal solution of the recording pipette by any detergent and avoiding the necessity of a separate chamber for cleaning. We showed that the patch-pipettes can be used consecutively at least ten times and that the cleaning process does not negatively impact neither the brain slices nor other patched neurons. This method, combined with automated patch-clamp, highly improves the throughput for single and especially multiple recordings.https://doi.org/10.1038/s41598-024-51837-7 |
| spellingShingle | Kevin Jehasse Jean-Sébastien Jouhanneau Sophie Wetz Alexander Schwedt James F. A. Poulet Peter Neumann-Raizel Björn M. Kampa Immediate reuse of patch-clamp pipettes after ultrasonic cleaning Scientific Reports |
| title | Immediate reuse of patch-clamp pipettes after ultrasonic cleaning |
| title_full | Immediate reuse of patch-clamp pipettes after ultrasonic cleaning |
| title_fullStr | Immediate reuse of patch-clamp pipettes after ultrasonic cleaning |
| title_full_unstemmed | Immediate reuse of patch-clamp pipettes after ultrasonic cleaning |
| title_short | Immediate reuse of patch-clamp pipettes after ultrasonic cleaning |
| title_sort | immediate reuse of patch clamp pipettes after ultrasonic cleaning |
| url | https://doi.org/10.1038/s41598-024-51837-7 |
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