Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts
Reconstruction of segmental bone defects by autologous bone grafting is still the standard of care but presents challenges including anatomical availability and potential donor site morbidity. The process of 3D bioprinting, the application of 3D printing for direct fabrication of living tissue, open...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/796 |
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| author | Anna-Klara Amler Patrick H. Dinkelborg Domenic Schlauch Jacob Spinnen Stefan Stich Roland Lauster Michael Sittinger Susanne Nahles Max Heiland Lutz Kloke Carsten Rendenbach Benedicta Beck-Broichsitter Tilo Dehne |
| author_facet | Anna-Klara Amler Patrick H. Dinkelborg Domenic Schlauch Jacob Spinnen Stefan Stich Roland Lauster Michael Sittinger Susanne Nahles Max Heiland Lutz Kloke Carsten Rendenbach Benedicta Beck-Broichsitter Tilo Dehne |
| author_sort | Anna-Klara Amler |
| collection | DOAJ |
| description | Reconstruction of segmental bone defects by autologous bone grafting is still the standard of care but presents challenges including anatomical availability and potential donor site morbidity. The process of 3D bioprinting, the application of 3D printing for direct fabrication of living tissue, opens new possibilities for highly personalized tissue implants, making it an appealing alternative to autologous bone grafts. One of the most crucial hurdles for the clinical application of 3D bioprinting is the choice of a suitable cell source, which should be minimally invasive, with high osteogenic potential, with fast, easy expansion. In this study, mesenchymal progenitor cells were isolated from clinically relevant human bone biopsy sites (explant cultures from alveolar bone, iliac crest and fibula; bone marrow aspirates; and periosteal bone shaving from the mastoid) and 3D bioprinted using projection-based stereolithography. Printed constructs were cultivated for 28 days and analyzed regarding their osteogenic potential by assessing viability, mineralization, and gene expression. While viability levels of all cell sources were comparable over the course of the cultivation, cells obtained by periosteal bone shaving showed higher mineralization of the print matrix, with gene expression data suggesting advanced osteogenic differentiation. These results indicate that periosteum-derived cells represent a highly promising cell source for translational bioprinting of bone tissue given their superior osteogenic potential as well as their minimally invasive obtainability. |
| first_indexed | 2024-03-09T04:44:51Z |
| format | Article |
| id | doaj.art-5526d70f95bc4b49ab122dcddf9383ab |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:44:51Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5526d70f95bc4b49ab122dcddf9383ab2023-12-03T13:16:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122279610.3390/ijms22020796Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone GraftsAnna-Klara Amler0Patrick H. Dinkelborg1Domenic Schlauch2Jacob Spinnen3Stefan Stich4Roland Lauster5Michael Sittinger6Susanne Nahles7Max Heiland8Lutz Kloke9Carsten Rendenbach10Benedicta Beck-Broichsitter11Tilo Dehne12Department of Medical Biotechnology, Technische Universität Berlin, 13355 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Oral and Maxillofacial Surgery, and Berlin Institute of Health, 13353 Berlin, GermanyDepartment of Medical Biotechnology, Technische Universität Berlin, 13355 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Rheumatology, and Berlin Institute of Health, 10117 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Rheumatology, and Berlin Institute of Health, 10117 Berlin, GermanyDepartment of Medical Biotechnology, Technische Universität Berlin, 13355 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Rheumatology, and Berlin Institute of Health, 10117 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Oral and Maxillofacial Surgery, and Berlin Institute of Health, 13353 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Oral and Maxillofacial Surgery, and Berlin Institute of Health, 13353 Berlin, GermanyCellbricks GmbH, 13355 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Oral and Maxillofacial Surgery, and Berlin Institute of Health, 13353 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Oral and Maxillofacial Surgery, and Berlin Institute of Health, 13353 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin, Department of Rheumatology, and Berlin Institute of Health, 10117 Berlin, GermanyReconstruction of segmental bone defects by autologous bone grafting is still the standard of care but presents challenges including anatomical availability and potential donor site morbidity. The process of 3D bioprinting, the application of 3D printing for direct fabrication of living tissue, opens new possibilities for highly personalized tissue implants, making it an appealing alternative to autologous bone grafts. One of the most crucial hurdles for the clinical application of 3D bioprinting is the choice of a suitable cell source, which should be minimally invasive, with high osteogenic potential, with fast, easy expansion. In this study, mesenchymal progenitor cells were isolated from clinically relevant human bone biopsy sites (explant cultures from alveolar bone, iliac crest and fibula; bone marrow aspirates; and periosteal bone shaving from the mastoid) and 3D bioprinted using projection-based stereolithography. Printed constructs were cultivated for 28 days and analyzed regarding their osteogenic potential by assessing viability, mineralization, and gene expression. While viability levels of all cell sources were comparable over the course of the cultivation, cells obtained by periosteal bone shaving showed higher mineralization of the print matrix, with gene expression data suggesting advanced osteogenic differentiation. These results indicate that periosteum-derived cells represent a highly promising cell source for translational bioprinting of bone tissue given their superior osteogenic potential as well as their minimally invasive obtainability.https://www.mdpi.com/1422-0067/22/2/796bioprintingtissue engineeringgelatin methacrylateregenerative medicinesegmental bone defectmesenchymal progenitor cell |
| spellingShingle | Anna-Klara Amler Patrick H. Dinkelborg Domenic Schlauch Jacob Spinnen Stefan Stich Roland Lauster Michael Sittinger Susanne Nahles Max Heiland Lutz Kloke Carsten Rendenbach Benedicta Beck-Broichsitter Tilo Dehne Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts International Journal of Molecular Sciences bioprinting tissue engineering gelatin methacrylate regenerative medicine segmental bone defect mesenchymal progenitor cell |
| title | Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts |
| title_full | Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts |
| title_fullStr | Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts |
| title_full_unstemmed | Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts |
| title_short | Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts |
| title_sort | comparison of the translational potential of human mesenchymal progenitor cells from different bone entities for autologous 3d bioprinted bone grafts |
| topic | bioprinting tissue engineering gelatin methacrylate regenerative medicine segmental bone defect mesenchymal progenitor cell |
| url | https://www.mdpi.com/1422-0067/22/2/796 |
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