Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats
Introduction: Diabetes is one of the most common metabolic diseases that is associated with high blood sugar and can cause disorders of the central nervous system. The purpose of the present study was to investigate the effect of a course of endurance activity on the levels of hippocampal Pannexin-1...
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2020-06-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/article-1-5017-en.html |
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| author | Mohammad Rami Mohammad Fathi Masoud Rahmati Mohammad Reza Tabandeh |
| author_facet | Mohammad Rami Mohammad Fathi Masoud Rahmati Mohammad Reza Tabandeh |
| author_sort | Mohammad Rami |
| collection | DOAJ |
| description | Introduction: Diabetes is one of the most common metabolic diseases that is associated with high blood sugar and can cause disorders of the central nervous system. The purpose of the present study was to investigate the effect of a course of endurance activity on the levels of hippocampal Pannexin-1 and NLRP-1 proteins in diabetic Wistar rats.
Methods: In this experimental study, 28 rats with 10 weeks of age and average weight of 245±9.4 g were randomly divided into 4 groups of 7: control diabetic, trained diabetic, healthy control and healthy trained. Intraperitoneal injection of streptozotocin (45 mg/kg) was used to induce diabetes. Then, the endurance training protocol was performed for 6 weeks. 24 h after the last training session, hippocampal tissue of rats was extracted and Pannexin-1 and NLRP-1 protein levels were measured by Western Blot Method. In this study, one-way analysis of variance and Tukey test as a follow-up test were used. Statistical analysis was performed using SPSS software version 18.
Results: The results showed that the expression of Pannexin-1 and NLRP-1 protein was significantly increased in diabetic rats (P<0.05). Moreover, the results of Pannexin-1 and NLRP-1 protein expression showed that the expression of both proteins decreased significantly after 6 weeks of endurance training in the training diabetic group compared to the control diabetic group (P <0.05).
Conclusion: Our findings confirmed the effects of hyperglycemia induced by endurance physical activity and consequently its effect on the decreased expression of Pannexin-1 and NLRP-1 proteins. Therefore, it seems that physical activity may play an important role in ameliorating neurodegenerative disorders in type 1 diabetic patients by reducing the expression of these proteins. |
| first_indexed | 2024-12-11T19:33:48Z |
| format | Article |
| id | doaj.art-5527c0dd93c24b98979e91103de403f2 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-11T19:33:48Z |
| publishDate | 2020-06-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-5527c0dd93c24b98979e91103de403f22022-12-22T00:53:12ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332020-06-0128223842398Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar RatsMohammad Rami0Mohammad Fathi1Masoud Rahmati2Mohammad Reza Tabandeh3 Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz Department of Physical Education, Faculty of Literature and Human Sciences, Lorestan University Department of Physical Education, Faculty of Literature and Human Sciences, Lorestan University Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine,Shahid Chamran University of Ahvaz, Ahvaz Introduction: Diabetes is one of the most common metabolic diseases that is associated with high blood sugar and can cause disorders of the central nervous system. The purpose of the present study was to investigate the effect of a course of endurance activity on the levels of hippocampal Pannexin-1 and NLRP-1 proteins in diabetic Wistar rats. Methods: In this experimental study, 28 rats with 10 weeks of age and average weight of 245±9.4 g were randomly divided into 4 groups of 7: control diabetic, trained diabetic, healthy control and healthy trained. Intraperitoneal injection of streptozotocin (45 mg/kg) was used to induce diabetes. Then, the endurance training protocol was performed for 6 weeks. 24 h after the last training session, hippocampal tissue of rats was extracted and Pannexin-1 and NLRP-1 protein levels were measured by Western Blot Method. In this study, one-way analysis of variance and Tukey test as a follow-up test were used. Statistical analysis was performed using SPSS software version 18. Results: The results showed that the expression of Pannexin-1 and NLRP-1 protein was significantly increased in diabetic rats (P<0.05). Moreover, the results of Pannexin-1 and NLRP-1 protein expression showed that the expression of both proteins decreased significantly after 6 weeks of endurance training in the training diabetic group compared to the control diabetic group (P <0.05). Conclusion: Our findings confirmed the effects of hyperglycemia induced by endurance physical activity and consequently its effect on the decreased expression of Pannexin-1 and NLRP-1 proteins. Therefore, it seems that physical activity may play an important role in ameliorating neurodegenerative disorders in type 1 diabetic patients by reducing the expression of these proteins.http://jssu.ssu.ac.ir/article-1-5017-en.htmldiabetesendurance traininghippocampus. |
| spellingShingle | Mohammad Rami Mohammad Fathi Masoud Rahmati Mohammad Reza Tabandeh Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd diabetes endurance training hippocampus. |
| title | Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats |
| title_full | Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats |
| title_fullStr | Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats |
| title_full_unstemmed | Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats |
| title_short | Effect of 6 Weeks Endurance Exercise on Hippocampal Pannexin-1 and NLRP-1 Protein Levels in Experimental Diabetic Male Wistar Rats |
| title_sort | effect of 6 weeks endurance exercise on hippocampal pannexin 1 and nlrp 1 protein levels in experimental diabetic male wistar rats |
| topic | diabetes endurance training hippocampus. |
| url | http://jssu.ssu.ac.ir/article-1-5017-en.html |
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