Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction
Nitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow withou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.758213/full |
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| author | Wanda Biała-Leonhard Laura Zanin Stefano Gottardi Rita de Brito Francisco Silvia Venuti Fabio Valentinuzzi Fabio Valentinuzzi Tanja Mimmo Stefano Cesco Barbara Bassin Enrico Martinoia Enrico Martinoia Roberto Pinton Michał Jasiński Michał Jasiński Nicola Tomasi |
| author_facet | Wanda Biała-Leonhard Laura Zanin Stefano Gottardi Rita de Brito Francisco Silvia Venuti Fabio Valentinuzzi Fabio Valentinuzzi Tanja Mimmo Stefano Cesco Barbara Bassin Enrico Martinoia Enrico Martinoia Roberto Pinton Michał Jasiński Michał Jasiński Nicola Tomasi |
| author_sort | Wanda Biała-Leonhard |
| collection | DOAJ |
| description | Nitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow without supply of N or P fertilizers. The legume-rhizobial symbiosis starts with root release of isoflavonoids that act as signaling molecules perceived by compatible bacteria. Subsequently, bacteria release nod factors, which induce signaling cascades allowing the formation of functional N-fixing nodules. We report here the identification and functional characterization of a plasma membrane-localized MATE-type transporter (LaMATE2) involved in the release of genistein from white lupin roots. The LaMATE2 expression in the root is upregulated under N deficiency as well as low phosphate availability, two nutritional deficiencies that induce the release of this isoflavonoid. LaMATE2 silencing reduced genistein efflux and even more the formation of symbiotic nodules, supporting the crucial role of LaMATE2 in isoflavonoid release and nodulation. Furthermore, silencing of LaMATE2 limited the P-solubilization activity of lupin root exudates. Transport assays in yeast vesicles demonstrated that LaMATE2 acts as a proton-driven isoflavonoid transporter. |
| first_indexed | 2024-12-19T04:25:24Z |
| format | Article |
| id | doaj.art-5527de94f54c44cc9c2ca5b9bd8bc5b2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T04:25:24Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5527de94f54c44cc9c2ca5b9bd8bc5b22022-12-21T20:36:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.758213758213Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic InteractionWanda Biała-Leonhard0Laura Zanin1Stefano Gottardi2Rita de Brito Francisco3Silvia Venuti4Fabio Valentinuzzi5Fabio Valentinuzzi6Tanja Mimmo7Stefano Cesco8Barbara Bassin9Enrico Martinoia10Enrico Martinoia11Roberto Pinton12Michał Jasiński13Michał Jasiński14Nicola Tomasi15Department of Plant Molecular Physiology, Polish Academy of Sciences, Institute of Bioorganic Chemistry, Poznań, PolandDipartimento di Scienze Agro-Alimentari, Ambientali e Animali, University of Udine, Udine, ItalyDipartimento di Scienze Agro-Alimentari, Ambientali e Animali, University of Udine, Udine, ItalyInstitute of Plant and Microbial Biology, University of Zurich, Zurich, SwitzerlandDipartimento di Scienze Agro-Alimentari, Ambientali e Animali, University of Udine, Udine, ItalyDipartimento di Scienze Agro-Alimentari, Ambientali e Animali, University of Udine, Udine, ItalyFaculty of Science and Technology, Free University of Bozen Bolzano, Bolzano, ItalyFaculty of Science and Technology, Free University of Bozen Bolzano, Bolzano, ItalyFaculty of Science and Technology, Free University of Bozen Bolzano, Bolzano, ItalyInstitute of Plant and Microbial Biology, University of Zurich, Zurich, SwitzerlandInstitute of Plant and Microbial Biology, University of Zurich, Zurich, SwitzerlandInternational Research Center for Environmental Membrane Biology, Foshan University, Foshan, ChinaDipartimento di Scienze Agro-Alimentari, Ambientali e Animali, University of Udine, Udine, ItalyDepartment of Plant Molecular Physiology, Polish Academy of Sciences, Institute of Bioorganic Chemistry, Poznań, PolandDepartment of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, PolandDipartimento di Scienze Agro-Alimentari, Ambientali e Animali, University of Udine, Udine, ItalyNitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow without supply of N or P fertilizers. The legume-rhizobial symbiosis starts with root release of isoflavonoids that act as signaling molecules perceived by compatible bacteria. Subsequently, bacteria release nod factors, which induce signaling cascades allowing the formation of functional N-fixing nodules. We report here the identification and functional characterization of a plasma membrane-localized MATE-type transporter (LaMATE2) involved in the release of genistein from white lupin roots. The LaMATE2 expression in the root is upregulated under N deficiency as well as low phosphate availability, two nutritional deficiencies that induce the release of this isoflavonoid. LaMATE2 silencing reduced genistein efflux and even more the formation of symbiotic nodules, supporting the crucial role of LaMATE2 in isoflavonoid release and nodulation. Furthermore, silencing of LaMATE2 limited the P-solubilization activity of lupin root exudates. Transport assays in yeast vesicles demonstrated that LaMATE2 acts as a proton-driven isoflavonoid transporter.https://www.frontiersin.org/articles/10.3389/fpls.2021.758213/fullBradyrhizobiumgenisteinLupinus albusMATE transporternitrogenphosphorus |
| spellingShingle | Wanda Biała-Leonhard Laura Zanin Stefano Gottardi Rita de Brito Francisco Silvia Venuti Fabio Valentinuzzi Fabio Valentinuzzi Tanja Mimmo Stefano Cesco Barbara Bassin Enrico Martinoia Enrico Martinoia Roberto Pinton Michał Jasiński Michał Jasiński Nicola Tomasi Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction Frontiers in Plant Science Bradyrhizobium genistein Lupinus albus MATE transporter nitrogen phosphorus |
| title | Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction |
| title_full | Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction |
| title_fullStr | Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction |
| title_full_unstemmed | Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction |
| title_short | Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction |
| title_sort | identification of an isoflavonoid transporter required for the nodule establishment of the rhizobium fabaceae symbiotic interaction |
| topic | Bradyrhizobium genistein Lupinus albus MATE transporter nitrogen phosphorus |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.758213/full |
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