Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China
Comparing gene expressions among parasitic plants infecting different host species can have significant implications for understanding host–parasite interactions. <i>Taxillus nigrans</i> is a common hemiparasitic species in Southwest China that parasitizes a variety of host species. Howe...
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MDPI AG
2022-06-01
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| author | Jingge Kuang Yufei Wang Kangshan Mao Richard Milne Mingcheng Wang Ning Miao |
| author_facet | Jingge Kuang Yufei Wang Kangshan Mao Richard Milne Mingcheng Wang Ning Miao |
| author_sort | Jingge Kuang |
| collection | DOAJ |
| description | Comparing gene expressions among parasitic plants infecting different host species can have significant implications for understanding host–parasite interactions. <i>Taxillus nigrans</i> is a common hemiparasitic species in Southwest China that parasitizes a variety of host species. However, a lack of nucleotide sequence data to date has hindered transcriptome-level research on <i>T. nigrans</i>. In this study, the transcriptomes of <i>T. nigrans</i> individuals parasitizing four typical host species (<i>Broussonetia papyrifera</i> (Bpap), a broad-leaved tree species; <i>Cryptomeria fortunei</i> (Cfor), a coniferous tree species; <i>Cinnamomum septentrionale</i> (Csep), an evergreen tree species; and <i>Ginkgo biloba</i> (Gbil), a deciduous-coniferous tree species) were sequenced, and the expression profiles and metabolic pathways were compared among hosts. A total of greater than 400 million reads were generated in nine cDNA libraries. These were de novo assembled into 293823 transcripts with an N50 value of 1790 bp. A large number of differentially expressed genes (DEGs) were identified when comparing <i>T. nigrans</i> individuals on different host species: Bpap vs. Cfor (1253 DEGs), Bpap vs. Csep (864), Bpap vs. Gbil (517), Cfor vs. Csep (259), Cfor vs. Gbil (95), and Csep vs. Gbil (40). Four hundred and fifteen unigenes were common to all six pairwise comparisons; these were primarily associated with Cytochrome P450 and environmental adaptation, as determined in a KEGG enrichment analysis. Unique unigenes were also identified, specific to Bpap vs. Cfor (808 unigenes), Bpap vs. Csep (329 unigenes), Bpap vs. Gbil (87 unigenes), Cfor vs. Csep (108 unigenes), Cfor vs. Gbil (32 unigenes), and Csep vs. Gbil comparisons (23 unigenes); partial unigenes were associated with the metabolism of terpenoids and polyketides regarding plant hormone signal transduction. Weighted gene co-expression network analysis (WGCNA) revealed four modules that were associated with the hosts. These results provide a foundation for further exploration of the detailed molecular mechanisms involved in plant parasitism. |
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| spelling | doaj.art-553218e065e24af0ba6eed5289b0527c2023-12-01T22:11:29ZengMDPI AGGenes2073-44252022-06-01137117310.3390/genes13071173Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest ChinaJingge Kuang0Yufei Wang1Kangshan Mao2Richard Milne3Mingcheng Wang4Ning Miao5Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaInstitute of Molecular Plant Sciences, The University of Edinburgh, Edinburgh EH9 3JH, UKInstitute for Advanced Study, Chengdu University, Chengdu 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaComparing gene expressions among parasitic plants infecting different host species can have significant implications for understanding host–parasite interactions. <i>Taxillus nigrans</i> is a common hemiparasitic species in Southwest China that parasitizes a variety of host species. However, a lack of nucleotide sequence data to date has hindered transcriptome-level research on <i>T. nigrans</i>. In this study, the transcriptomes of <i>T. nigrans</i> individuals parasitizing four typical host species (<i>Broussonetia papyrifera</i> (Bpap), a broad-leaved tree species; <i>Cryptomeria fortunei</i> (Cfor), a coniferous tree species; <i>Cinnamomum septentrionale</i> (Csep), an evergreen tree species; and <i>Ginkgo biloba</i> (Gbil), a deciduous-coniferous tree species) were sequenced, and the expression profiles and metabolic pathways were compared among hosts. A total of greater than 400 million reads were generated in nine cDNA libraries. These were de novo assembled into 293823 transcripts with an N50 value of 1790 bp. A large number of differentially expressed genes (DEGs) were identified when comparing <i>T. nigrans</i> individuals on different host species: Bpap vs. Cfor (1253 DEGs), Bpap vs. Csep (864), Bpap vs. Gbil (517), Cfor vs. Csep (259), Cfor vs. Gbil (95), and Csep vs. Gbil (40). Four hundred and fifteen unigenes were common to all six pairwise comparisons; these were primarily associated with Cytochrome P450 and environmental adaptation, as determined in a KEGG enrichment analysis. Unique unigenes were also identified, specific to Bpap vs. Cfor (808 unigenes), Bpap vs. Csep (329 unigenes), Bpap vs. Gbil (87 unigenes), Cfor vs. Csep (108 unigenes), Cfor vs. Gbil (32 unigenes), and Csep vs. Gbil comparisons (23 unigenes); partial unigenes were associated with the metabolism of terpenoids and polyketides regarding plant hormone signal transduction. Weighted gene co-expression network analysis (WGCNA) revealed four modules that were associated with the hosts. These results provide a foundation for further exploration of the detailed molecular mechanisms involved in plant parasitism.https://www.mdpi.com/2073-4425/13/7/1173mistletoehost selectionhemiparasite<i>Taxillus nigrans</i>RNA-seqWGCNA |
| spellingShingle | Jingge Kuang Yufei Wang Kangshan Mao Richard Milne Mingcheng Wang Ning Miao Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China Genes mistletoe host selection hemiparasite <i>Taxillus nigrans</i> RNA-seq WGCNA |
| title | Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China |
| title_full | Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China |
| title_fullStr | Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China |
| title_full_unstemmed | Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China |
| title_short | Transcriptome Profiling of a Common Mistletoe Species Parasitizing Four Typical Host Species in Urban Southwest China |
| title_sort | transcriptome profiling of a common mistletoe species parasitizing four typical host species in urban southwest china |
| topic | mistletoe host selection hemiparasite <i>Taxillus nigrans</i> RNA-seq WGCNA |
| url | https://www.mdpi.com/2073-4425/13/7/1173 |
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