Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues
Benzylisoquinoline alkaloids in lotus (Nelumbo nucifera) seed plumules and leaves exhibit significant tissue specificity for their pharmacological effects and potential nutritional properties. Herein, 46 benzylisoquinoline alkaloids were identified via UPLC-QTOF-HRMS, of which 9 were annotated as gl...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023033455 |
| _version_ | 1827940899324166144 |
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| author | Chenyang Hao Wei Yang Gangqiang Dong Yuetong Yu Yan Liu Jun Zhang Yongping Zhu Xiaolu Wei Sha Chen |
| author_facet | Chenyang Hao Wei Yang Gangqiang Dong Yuetong Yu Yan Liu Jun Zhang Yongping Zhu Xiaolu Wei Sha Chen |
| author_sort | Chenyang Hao |
| collection | DOAJ |
| description | Benzylisoquinoline alkaloids in lotus (Nelumbo nucifera) seed plumules and leaves exhibit significant tissue specificity for their pharmacological effects and potential nutritional properties. Herein, 46 benzylisoquinoline alkaloids were identified via UPLC-QTOF-HRMS, of which 9 were annotated as glycosylated monobenzylisoquinoline alkaloids concentrated in the seed plumules. The spatial distribution of targeted benzylisoquinoline alkaloids in leaves, seed plumules, and milky sap was determined via MALDI–MSI. Furthermore, 37 Nelumbo cultivars were investigated using targeted metabolomics to provide insights into functional tea development. While aporphine alkaloids comprised the main compounds present in lotus leaves, bisbenzylisoquinoline alkaloids were the main compounds in lotus plumules, where glycosylation primarily occurs. These findings can help understand the distribution of benzylisoquinoline alkaloids in lotus tissue and the directional breeding of varieties enriched with specific chemical functional groups for nutritional and pharmacological applications. |
| first_indexed | 2024-03-13T09:29:08Z |
| format | Article |
| id | doaj.art-5533765682a545e0a834dab7eb761264 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T09:29:08Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-5533765682a545e0a834dab7eb7612642023-05-26T04:21:45ZengElsevierHeliyon2405-84402023-06-0196e16138Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissuesChenyang Hao0Wei Yang1Gangqiang Dong2Yuetong Yu3Yan Liu4Jun Zhang5Yongping Zhu6Xiaolu Wei7Sha Chen8Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, ChinaAmway (China) Botanical R&D Center, Wuxi 214115, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China; Corresponding author.Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China; Corresponding author.Benzylisoquinoline alkaloids in lotus (Nelumbo nucifera) seed plumules and leaves exhibit significant tissue specificity for their pharmacological effects and potential nutritional properties. Herein, 46 benzylisoquinoline alkaloids were identified via UPLC-QTOF-HRMS, of which 9 were annotated as glycosylated monobenzylisoquinoline alkaloids concentrated in the seed plumules. The spatial distribution of targeted benzylisoquinoline alkaloids in leaves, seed plumules, and milky sap was determined via MALDI–MSI. Furthermore, 37 Nelumbo cultivars were investigated using targeted metabolomics to provide insights into functional tea development. While aporphine alkaloids comprised the main compounds present in lotus leaves, bisbenzylisoquinoline alkaloids were the main compounds in lotus plumules, where glycosylation primarily occurs. These findings can help understand the distribution of benzylisoquinoline alkaloids in lotus tissue and the directional breeding of varieties enriched with specific chemical functional groups for nutritional and pharmacological applications.http://www.sciencedirect.com/science/article/pii/S2405844023033455In situ detectionMetabolomicsBenzylisoquinoline alkaloidsMALDI–MSIUPLC-QTOF-HRMS |
| spellingShingle | Chenyang Hao Wei Yang Gangqiang Dong Yuetong Yu Yan Liu Jun Zhang Yongping Zhu Xiaolu Wei Sha Chen Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues Heliyon In situ detection Metabolomics Benzylisoquinoline alkaloids MALDI–MSI UPLC-QTOF-HRMS |
| title | Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues |
| title_full | Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues |
| title_fullStr | Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues |
| title_full_unstemmed | Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues |
| title_short | Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues |
| title_sort | visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues |
| topic | In situ detection Metabolomics Benzylisoquinoline alkaloids MALDI–MSI UPLC-QTOF-HRMS |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023033455 |
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