Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells
Abstract Objectives Breast cancer cell growth and proliferation requires lipids for energy production, cell membrane synthesis, or as signaling molecules. Lipids can be delivered to cells by lipoprotein lipase (LPL), an extracellular lipase that hydrolyzes triacylglycerols and phospholipids from lip...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-021-05728-z |
| _version_ | 1819110804873543680 |
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| author | Alexandria J. Tobin Nicholas P. Noel Sherri L. Christian Robert J. Brown |
| author_facet | Alexandria J. Tobin Nicholas P. Noel Sherri L. Christian Robert J. Brown |
| author_sort | Alexandria J. Tobin |
| collection | DOAJ |
| description | Abstract Objectives Breast cancer cell growth and proliferation requires lipids for energy production, cell membrane synthesis, or as signaling molecules. Lipids can be delivered to cells by lipoprotein lipase (LPL), an extracellular lipase that hydrolyzes triacylglycerols and phospholipids from lipoproteins, that is expressed by adipose tissue and some breast cancer cell lines. Studies have shown that lipoprotein hydrolysis products induce pro-inflammatory cytokine secretion by endothelial cells. Thus, our objective was to determine if hydrolysis products generated by LPL from total lipoproteins can also promote pro-inflammatory cytokine secretion from breast cancer cells. Results Using cytokine arrays, we found that MDA-MB-231 cells increased secretion of seven cytokines in response to treatment with lipoprotein hydrolysis products. In contrast, MCF-7 cells showed decreased secretion of two cytokines. Expanding the analysis to additional cell lines by ELISA, we found increased secretion of TNF-α and IL-6 by MDA-MB-468 cells, and increased secretion of IL-4 by MDA-MB-468 and SKBR3 cells. The changes to cytokine secretion profiles of the breast cancer cell types examined, including the non-cancerous MCF-10a breast cells, were independent of increased cell metabolic activity. These results provide information on how lipoprotein hydrolysis products within the tumor microenvironment might affect breast cancer cell viability and progression. |
| first_indexed | 2024-12-22T03:47:33Z |
| format | Article |
| id | doaj.art-55357eb564e5438d9371aaf53f519679 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-22T03:47:33Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-55357eb564e5438d9371aaf53f5196792022-12-21T18:40:06ZengBMCBMC Research Notes1756-05002021-08-011411610.1186/s13104-021-05728-zLipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cellsAlexandria J. Tobin0Nicholas P. Noel1Sherri L. Christian2Robert J. Brown3Department of Biochemistry, Memorial University of NewfoundlandDepartment of Biochemistry, Memorial University of NewfoundlandDepartment of Biochemistry, Memorial University of NewfoundlandDepartment of Biochemistry, Memorial University of NewfoundlandAbstract Objectives Breast cancer cell growth and proliferation requires lipids for energy production, cell membrane synthesis, or as signaling molecules. Lipids can be delivered to cells by lipoprotein lipase (LPL), an extracellular lipase that hydrolyzes triacylglycerols and phospholipids from lipoproteins, that is expressed by adipose tissue and some breast cancer cell lines. Studies have shown that lipoprotein hydrolysis products induce pro-inflammatory cytokine secretion by endothelial cells. Thus, our objective was to determine if hydrolysis products generated by LPL from total lipoproteins can also promote pro-inflammatory cytokine secretion from breast cancer cells. Results Using cytokine arrays, we found that MDA-MB-231 cells increased secretion of seven cytokines in response to treatment with lipoprotein hydrolysis products. In contrast, MCF-7 cells showed decreased secretion of two cytokines. Expanding the analysis to additional cell lines by ELISA, we found increased secretion of TNF-α and IL-6 by MDA-MB-468 cells, and increased secretion of IL-4 by MDA-MB-468 and SKBR3 cells. The changes to cytokine secretion profiles of the breast cancer cell types examined, including the non-cancerous MCF-10a breast cells, were independent of increased cell metabolic activity. These results provide information on how lipoprotein hydrolysis products within the tumor microenvironment might affect breast cancer cell viability and progression.https://doi.org/10.1186/s13104-021-05728-zLipoprotein lipaseLipoproteinsBreast cancerCytokinesMetabolic activityAntibody arrays |
| spellingShingle | Alexandria J. Tobin Nicholas P. Noel Sherri L. Christian Robert J. Brown Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells BMC Research Notes Lipoprotein lipase Lipoproteins Breast cancer Cytokines Metabolic activity Antibody arrays |
| title | Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells |
| title_full | Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells |
| title_fullStr | Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells |
| title_full_unstemmed | Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells |
| title_short | Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells |
| title_sort | lipoprotein lipase hydrolysis products induce pro inflammatory cytokine expression in triple negative breast cancer cells |
| topic | Lipoprotein lipase Lipoproteins Breast cancer Cytokines Metabolic activity Antibody arrays |
| url | https://doi.org/10.1186/s13104-021-05728-z |
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