Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging
The main trend of current research in neurodegenerative diseases (NDDs) is directed towards the discovery of novel biomarkers for disease diagnostics and progression. The pathological features of NDDs suggest that diagnostic markers can be found in peripheral fluids and cells. Herein, we investigate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/10/1500 |
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| author | Svetla Todinova Sashka Krumova Desislava Bogdanova Avgustina Danailova Elena Zlatareva Nikolay Kalaydzhiev Ariana Langari Ivan Milanov Stefka G. Taneva |
| author_facet | Svetla Todinova Sashka Krumova Desislava Bogdanova Avgustina Danailova Elena Zlatareva Nikolay Kalaydzhiev Ariana Langari Ivan Milanov Stefka G. Taneva |
| author_sort | Svetla Todinova |
| collection | DOAJ |
| description | The main trend of current research in neurodegenerative diseases (NDDs) is directed towards the discovery of novel biomarkers for disease diagnostics and progression. The pathological features of NDDs suggest that diagnostic markers can be found in peripheral fluids and cells. Herein, we investigated the thermodynamic behavior of the peripheral red blood cells (RBCs) derived from patients diagnosed with three common NDDs—Parkinson’s disease (PD), Alzheimer’s disease (AD), and amyotrophic lateral sclerosis (ALS) and compared it with that of healthy individuals, evaluating both fresh and aged RBCs. We established that NDDs can be differentiated from the normal healthy state on the basis of the variation in the thermodynamic parameters of the unfolding of major RBCs proteins—the cytoplasmic hemoglobin (Hb) and the membrane Band 3 (B3) protein. A common feature of NDDs is the higher thermal stability of both Hb and B3 proteins along the RBCs aging, while the calorimetric enthalpy can distinguish PD from ALS and AD. Our data provide insights into the RBCs thermodynamic behavior in two complex and tightly related phenomena—neurodegenerative pathologies and aging, and it suggests that the determined thermodynamic parameters are fingerprints of the altered conformation of Hb and B3 protein and modified RBCs’ aging in the studied NDDs. |
| first_indexed | 2024-03-10T06:42:21Z |
| format | Article |
| id | doaj.art-553652749f6246b6aaba9f6098632f3a |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T06:42:21Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-553652749f6246b6aaba9f6098632f3a2023-11-22T17:34:33ZengMDPI AGBiomolecules2218-273X2021-10-011110150010.3390/biom11101500Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and AgingSvetla Todinova0Sashka Krumova1Desislava Bogdanova2Avgustina Danailova3Elena Zlatareva4Nikolay Kalaydzhiev5Ariana Langari6Ivan Milanov7Stefka G. Taneva8Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev, 1113 Sofia, BulgariaDepartment of Neurology, University Multiprofile Hospital for Active Treatment in Neurology and Psychiatry Sv. Naum, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev, 1113 Sofia, BulgariaDepartment of Neurology, University Multiprofile Hospital for Active Treatment in Neurology and Psychiatry Sv. Naum, 1113 Sofia, BulgariaDepartment of Neurology, University Multiprofile Hospital for Active Treatment in Neurology and Psychiatry Sv. Naum, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev, 1113 Sofia, BulgariaDepartment of Neurology, University Multiprofile Hospital for Active Treatment in Neurology and Psychiatry Sv. Naum, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev, 1113 Sofia, BulgariaThe main trend of current research in neurodegenerative diseases (NDDs) is directed towards the discovery of novel biomarkers for disease diagnostics and progression. The pathological features of NDDs suggest that diagnostic markers can be found in peripheral fluids and cells. Herein, we investigated the thermodynamic behavior of the peripheral red blood cells (RBCs) derived from patients diagnosed with three common NDDs—Parkinson’s disease (PD), Alzheimer’s disease (AD), and amyotrophic lateral sclerosis (ALS) and compared it with that of healthy individuals, evaluating both fresh and aged RBCs. We established that NDDs can be differentiated from the normal healthy state on the basis of the variation in the thermodynamic parameters of the unfolding of major RBCs proteins—the cytoplasmic hemoglobin (Hb) and the membrane Band 3 (B3) protein. A common feature of NDDs is the higher thermal stability of both Hb and B3 proteins along the RBCs aging, while the calorimetric enthalpy can distinguish PD from ALS and AD. Our data provide insights into the RBCs thermodynamic behavior in two complex and tightly related phenomena—neurodegenerative pathologies and aging, and it suggests that the determined thermodynamic parameters are fingerprints of the altered conformation of Hb and B3 protein and modified RBCs’ aging in the studied NDDs.https://www.mdpi.com/2218-273X/11/10/1500neurodegenerative diseasesred blood cellshemoglobinBand 3 proteindifferential scanning calorimetrythermal transitions |
| spellingShingle | Svetla Todinova Sashka Krumova Desislava Bogdanova Avgustina Danailova Elena Zlatareva Nikolay Kalaydzhiev Ariana Langari Ivan Milanov Stefka G. Taneva Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging Biomolecules neurodegenerative diseases red blood cells hemoglobin Band 3 protein differential scanning calorimetry thermal transitions |
| title | Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging |
| title_full | Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging |
| title_fullStr | Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging |
| title_full_unstemmed | Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging |
| title_short | Red Blood Cells’ Thermodynamic Behavior in Neurodegenerative Pathologies and Aging |
| title_sort | red blood cells thermodynamic behavior in neurodegenerative pathologies and aging |
| topic | neurodegenerative diseases red blood cells hemoglobin Band 3 protein differential scanning calorimetry thermal transitions |
| url | https://www.mdpi.com/2218-273X/11/10/1500 |
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