Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources
X-ray free electron lasers (XFELs) now routinely produce millijoule level pulses of x-ray photons with tens of femtoseconds duration. Such x-ray intensities gave rise to the idea that weakly scattering particles—perhaps single biomolecules or viruses—could be investigated free of radiation damage. H...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC and ACA
2020-07-01
|
| Series: | Structural Dynamics |
| Online Access: | http://dx.doi.org/10.1063/4.0000024 |
| _version_ | 1819110024024162304 |
|---|---|
| author | Johan Bielecki Filipe R. N. C. Maia Adrian P. Mancuso |
| author_facet | Johan Bielecki Filipe R. N. C. Maia Adrian P. Mancuso |
| author_sort | Johan Bielecki |
| collection | DOAJ |
| description | X-ray free electron lasers (XFELs) now routinely produce millijoule level pulses of x-ray photons with tens of femtoseconds duration. Such x-ray intensities gave rise to the idea that weakly scattering particles—perhaps single biomolecules or viruses—could be investigated free of radiation damage. Here, we examine elements from the past decade of so-called single particle imaging with hard XFELs. We look at the progress made to date and identify some future possible directions for the field. In particular, we summarize the presently achieved resolutions as well as identifying the bottlenecks and enabling technologies to future resolution improvement, which in turn enables application to samples of scientific interest. |
| first_indexed | 2024-12-22T03:35:08Z |
| format | Article |
| id | doaj.art-5538b39980f24db78249d03c8896a133 |
| institution | Directory Open Access Journal |
| issn | 2329-7778 |
| language | English |
| last_indexed | 2024-12-22T03:35:08Z |
| publishDate | 2020-07-01 |
| publisher | AIP Publishing LLC and ACA |
| record_format | Article |
| series | Structural Dynamics |
| spelling | doaj.art-5538b39980f24db78249d03c8896a1332022-12-21T18:40:24ZengAIP Publishing LLC and ACAStructural Dynamics2329-77782020-07-0174040901040901-510.1063/4.0000024Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sourcesJohan Bielecki0Filipe R. N. C. Maia1Adrian P. Mancuso2 European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden European XFEL, Holzkoppel 4, 22869 Schenefeld, GermanyX-ray free electron lasers (XFELs) now routinely produce millijoule level pulses of x-ray photons with tens of femtoseconds duration. Such x-ray intensities gave rise to the idea that weakly scattering particles—perhaps single biomolecules or viruses—could be investigated free of radiation damage. Here, we examine elements from the past decade of so-called single particle imaging with hard XFELs. We look at the progress made to date and identify some future possible directions for the field. In particular, we summarize the presently achieved resolutions as well as identifying the bottlenecks and enabling technologies to future resolution improvement, which in turn enables application to samples of scientific interest.http://dx.doi.org/10.1063/4.0000024 |
| spellingShingle | Johan Bielecki Filipe R. N. C. Maia Adrian P. Mancuso Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources Structural Dynamics |
| title | Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources |
| title_full | Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources |
| title_fullStr | Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources |
| title_full_unstemmed | Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources |
| title_short | Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources |
| title_sort | perspectives on single particle imaging with x rays at the advent of high repetition rate x ray free electron laser sources |
| url | http://dx.doi.org/10.1063/4.0000024 |
| work_keys_str_mv | AT johanbielecki perspectivesonsingleparticleimagingwithxraysattheadventofhighrepetitionratexrayfreeelectronlasersources AT filiperncmaia perspectivesonsingleparticleimagingwithxraysattheadventofhighrepetitionratexrayfreeelectronlasersources AT adrianpmancuso perspectivesonsingleparticleimagingwithxraysattheadventofhighrepetitionratexrayfreeelectronlasersources |