Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells
Background This study was performed to provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calci...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Yeungnam University College of Medicine
2020-07-01
|
| Series: | Yeungnam University Journal of Medicine |
| Subjects: | |
| Online Access: | http://www.e-yujm.org/upload/pdf/yujm-2020-00248.pdf |
| _version_ | 1818522972406677504 |
|---|---|
| author | Sohee Kang |
| author_facet | Sohee Kang |
| author_sort | Sohee Kang |
| collection | DOAJ |
| description | Background This study was performed to provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calcium silicate-based pulp capping materials (ProRoot MTA [PR], Biodentine [BD], and TheraCal LC [TC]) in human dental pulp cells (HDPCs). Methods Specimens of test materials were placed in deionized water for various incubation times to measure the pH variation and the concentration of calcium released. The morphology of HDPCs cultured on the specimens was examined using a confocal laser scanning microscope (CLSM). Alizarin red S staining and alkaline phosphatase assays were used to evaluate mineralization-inducing potentials of the capping materials. Results BD showed the highest calcium release in all test periods, followed by PR and TC. (p<0.05). All experimental groups showed high alkalinity after 1 day, except at 14 days. BD showed the highest cell viability compared with PR and TC after 1 and 3 days, while TC showed the lowest value (p<0.05). The CLSM analysis showed that cells were well adhered and expressed actin filaments for all pulp capping materials. Mineralization by PR and BD groups was higher than that by TC group based on alizarin red S staining. BD showed significantly higher alkaline phosphatase activity than PR and TC, while TC showed the lowest value (p<0.05). Conclusion Within the limitations of the in vitro study, BD had higher mineralization-inducing potential than PR and TC. |
| first_indexed | 2024-12-11T05:39:39Z |
| format | Article |
| id | doaj.art-553dccc382ce4b439f4a20723835be66 |
| institution | Directory Open Access Journal |
| issn | 2384-0293 |
| language | English |
| last_indexed | 2024-12-11T05:39:39Z |
| publishDate | 2020-07-01 |
| publisher | Yeungnam University College of Medicine |
| record_format | Article |
| series | Yeungnam University Journal of Medicine |
| spelling | doaj.art-553dccc382ce4b439f4a20723835be662022-12-22T01:19:10ZengYeungnam University College of MedicineYeungnam University Journal of Medicine2384-02932020-07-0137321722510.12701/yujm.2020.002482491Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cellsSohee KangBackground This study was performed to provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calcium silicate-based pulp capping materials (ProRoot MTA [PR], Biodentine [BD], and TheraCal LC [TC]) in human dental pulp cells (HDPCs). Methods Specimens of test materials were placed in deionized water for various incubation times to measure the pH variation and the concentration of calcium released. The morphology of HDPCs cultured on the specimens was examined using a confocal laser scanning microscope (CLSM). Alizarin red S staining and alkaline phosphatase assays were used to evaluate mineralization-inducing potentials of the capping materials. Results BD showed the highest calcium release in all test periods, followed by PR and TC. (p<0.05). All experimental groups showed high alkalinity after 1 day, except at 14 days. BD showed the highest cell viability compared with PR and TC after 1 and 3 days, while TC showed the lowest value (p<0.05). The CLSM analysis showed that cells were well adhered and expressed actin filaments for all pulp capping materials. Mineralization by PR and BD groups was higher than that by TC group based on alizarin red S staining. BD showed significantly higher alkaline phosphatase activity than PR and TC, while TC showed the lowest value (p<0.05). Conclusion Within the limitations of the in vitro study, BD had higher mineralization-inducing potential than PR and TC.http://www.e-yujm.org/upload/pdf/yujm-2020-00248.pdfbiocompatibility testingdental materialdental pulp cappingdentin |
| spellingShingle | Sohee Kang Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells Yeungnam University Journal of Medicine biocompatibility testing dental material dental pulp capping dentin |
| title | Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells |
| title_full | Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells |
| title_fullStr | Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells |
| title_full_unstemmed | Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells |
| title_short | Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells |
| title_sort | mineralization inducing potentials of calcium silicate based pulp capping materials in human dental pulp cells |
| topic | biocompatibility testing dental material dental pulp capping dentin |
| url | http://www.e-yujm.org/upload/pdf/yujm-2020-00248.pdf |
| work_keys_str_mv | AT soheekang mineralizationinducingpotentialsofcalciumsilicatebasedpulpcappingmaterialsinhumandentalpulpcells |