Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression
Ciliated cell markers expressed in epithelial ovarian cancers (EOC) are associated with improved survival. We examined the distribution of cells expressing ciliated cell markers in various EOC histologies and stages. Immunohistochemistry and/or multiplex immunofluorescence were used to determine the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/24/4009 |
| _version_ | 1797460939866374144 |
|---|---|
| author | Michael T. Richardson Maria Sol Recouvreux Beth Y. Karlan Ann E. Walts Sandra Orsulic |
| author_facet | Michael T. Richardson Maria Sol Recouvreux Beth Y. Karlan Ann E. Walts Sandra Orsulic |
| author_sort | Michael T. Richardson |
| collection | DOAJ |
| description | Ciliated cell markers expressed in epithelial ovarian cancers (EOC) are associated with improved survival. We examined the distribution of cells expressing ciliated cell markers in various EOC histologies and stages. Immunohistochemistry and/or multiplex immunofluorescence were used to determine the expression of FOXJ1 and/or CAPS (ciliated cell markers) in tissue microarrays including 4 normal fallopian tubes, 6 normal endometria, 16 cystadenomas, 25 borderline tumors, 21 low-grade carcinomas, and 118 high-grade carcinomas (HGSOC) (46 serous, 29 endometrioid, 30 clear cell, 13 mucinous). CAPS+ cells were observed in normal fallopian tubes and endometria and in ~85% of serous benign and borderline tumors and low-grade carcinomas but only in <40% of HGSOC. mRNA data from an independent cohort showed higher FOXJ1 and CAPS expression in serous borderline tumors and low-grade carcinomas compared to HGSOC. In HGSOC, ciliated cell-positive markers were observed in 52% primary tumors compared to 26% of patient-matched synchronous metastases, and 24% metachronous metastases (<i>p</i> = 0.009). mRNA data from an independent HGSOC cohort showed lower levels of CAPS in metastases than in primary tumors (<i>p</i> = 0.03). Overall, the study revealed that ciliated cells were less common in mucinous EOC, the percentage of ciliated cell marker-positive cases decreased with increasing grade, and the percentage of ciliated cells decreased in HGSOC metastases compared to patient-matched primary tumors. |
| first_indexed | 2024-03-09T17:13:18Z |
| format | Article |
| id | doaj.art-553ebd3328ec4095b84aeae9faa0bc79 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T17:13:18Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-553ebd3328ec4095b84aeae9faa0bc792023-11-24T13:54:16ZengMDPI AGCells2073-44092022-12-011124400910.3390/cells11244009Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease ProgressionMichael T. Richardson0Maria Sol Recouvreux1Beth Y. Karlan2Ann E. Walts3Sandra Orsulic4Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USADepartment of Obstetrics and Gynecology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USADepartment of Obstetrics and Gynecology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USADepartment of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USADepartment of Obstetrics and Gynecology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USACiliated cell markers expressed in epithelial ovarian cancers (EOC) are associated with improved survival. We examined the distribution of cells expressing ciliated cell markers in various EOC histologies and stages. Immunohistochemistry and/or multiplex immunofluorescence were used to determine the expression of FOXJ1 and/or CAPS (ciliated cell markers) in tissue microarrays including 4 normal fallopian tubes, 6 normal endometria, 16 cystadenomas, 25 borderline tumors, 21 low-grade carcinomas, and 118 high-grade carcinomas (HGSOC) (46 serous, 29 endometrioid, 30 clear cell, 13 mucinous). CAPS+ cells were observed in normal fallopian tubes and endometria and in ~85% of serous benign and borderline tumors and low-grade carcinomas but only in <40% of HGSOC. mRNA data from an independent cohort showed higher FOXJ1 and CAPS expression in serous borderline tumors and low-grade carcinomas compared to HGSOC. In HGSOC, ciliated cell-positive markers were observed in 52% primary tumors compared to 26% of patient-matched synchronous metastases, and 24% metachronous metastases (<i>p</i> = 0.009). mRNA data from an independent HGSOC cohort showed lower levels of CAPS in metastases than in primary tumors (<i>p</i> = 0.03). Overall, the study revealed that ciliated cells were less common in mucinous EOC, the percentage of ciliated cell marker-positive cases decreased with increasing grade, and the percentage of ciliated cells decreased in HGSOC metastases compared to patient-matched primary tumors.https://www.mdpi.com/2073-4409/11/24/4009fallopian tubeciliated cellssecretory cellsovarian cancerserousmucinous |
| spellingShingle | Michael T. Richardson Maria Sol Recouvreux Beth Y. Karlan Ann E. Walts Sandra Orsulic Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression Cells fallopian tube ciliated cells secretory cells ovarian cancer serous mucinous |
| title | Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression |
| title_full | Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression |
| title_fullStr | Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression |
| title_full_unstemmed | Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression |
| title_short | Ciliated Cells in Ovarian Cancer Decrease with Increasing Tumor Grade and Disease Progression |
| title_sort | ciliated cells in ovarian cancer decrease with increasing tumor grade and disease progression |
| topic | fallopian tube ciliated cells secretory cells ovarian cancer serous mucinous |
| url | https://www.mdpi.com/2073-4409/11/24/4009 |
| work_keys_str_mv | AT michaeltrichardson ciliatedcellsinovariancancerdecreasewithincreasingtumorgradeanddiseaseprogression AT mariasolrecouvreux ciliatedcellsinovariancancerdecreasewithincreasingtumorgradeanddiseaseprogression AT bethykarlan ciliatedcellsinovariancancerdecreasewithincreasingtumorgradeanddiseaseprogression AT annewalts ciliatedcellsinovariancancerdecreasewithincreasingtumorgradeanddiseaseprogression AT sandraorsulic ciliatedcellsinovariancancerdecreasewithincreasingtumorgradeanddiseaseprogression |