Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity
In this paper instrumental methods of carbon dioxide (CO2) detection in biological material were compared. Using cis-[Cr(C2O4)(pm)(OH2)2]+ cation as a specific molecular biosensor and the stopped-flow technique the concentrations of CO2 released from the cell culture medium as one of final products...
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MDPI AG
2008-08-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/8/8/4487/ |
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| author | Lech Chmurzyński Michał Woźniak Aleksandra Dąbrowska Agnieszka Chylewska Michał Szkatuła Dagmara Jacewicz |
| author_facet | Lech Chmurzyński Michał Woźniak Aleksandra Dąbrowska Agnieszka Chylewska Michał Szkatuła Dagmara Jacewicz |
| author_sort | Lech Chmurzyński |
| collection | DOAJ |
| description | In this paper instrumental methods of carbon dioxide (CO2) detection in biological material were compared. Using cis-[Cr(C2O4)(pm)(OH2)2]+ cation as a specific molecular biosensor and the stopped-flow technique the concentrations of CO2 released from the cell culture medium as one of final products of pyruvate decomposition caused by hydrogen peroxide were determined. To prove the usefulness of our method of CO2 assessment in the case of biological samples we investigated protective properties of exogenous pyruvate in cultured osteosarcoma 143B cells exposed to 1 mM hydrogen peroxide (H2O2) added directly to culture medium. Pyruvic acid is well known scavenger of H2O2 and, moreover, a molecule which is recognized as one of the major mediator of oxidative stress detected in many diseases and pathological situations like ischemiareperfusion states. The pyruvate's antioxidant activity is described as its rapid reaction with H2O2,which causes nonenzymatic decarboxylation of pyruvate and releases of CO2, water and acetate as final products. In this work for the first time we have correlated the concentration of CO2 dissolved in culture medium with pyruvate's oxidant-scavenging abilities. Moreover, the kinetics of the reaction between aqueous solution of CO2 and coordinate ion, cis-[Cr(C2O4)(pm)(OH2)2]+ was analysed. The results obtained enabled determination of the number of steps of the reaction studied. Based on the kinetic equations, rate constants were determined for each step. |
| first_indexed | 2024-04-11T22:09:57Z |
| format | Article |
| id | doaj.art-553f80a459a14325889fe1289540495d |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:09:57Z |
| publishDate | 2008-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-553f80a459a14325889fe1289540495d2022-12-22T04:00:36ZengMDPI AGSensors1424-82202008-08-018844874504Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated CytotoxityLech ChmurzyńskiMichał WoźniakAleksandra DąbrowskaAgnieszka ChylewskaMichał SzkatułaDagmara JacewiczIn this paper instrumental methods of carbon dioxide (CO2) detection in biological material were compared. Using cis-[Cr(C2O4)(pm)(OH2)2]+ cation as a specific molecular biosensor and the stopped-flow technique the concentrations of CO2 released from the cell culture medium as one of final products of pyruvate decomposition caused by hydrogen peroxide were determined. To prove the usefulness of our method of CO2 assessment in the case of biological samples we investigated protective properties of exogenous pyruvate in cultured osteosarcoma 143B cells exposed to 1 mM hydrogen peroxide (H2O2) added directly to culture medium. Pyruvic acid is well known scavenger of H2O2 and, moreover, a molecule which is recognized as one of the major mediator of oxidative stress detected in many diseases and pathological situations like ischemiareperfusion states. The pyruvate's antioxidant activity is described as its rapid reaction with H2O2,which causes nonenzymatic decarboxylation of pyruvate and releases of CO2, water and acetate as final products. In this work for the first time we have correlated the concentration of CO2 dissolved in culture medium with pyruvate's oxidant-scavenging abilities. Moreover, the kinetics of the reaction between aqueous solution of CO2 and coordinate ion, cis-[Cr(C2O4)(pm)(OH2)2]+ was analysed. The results obtained enabled determination of the number of steps of the reaction studied. Based on the kinetic equations, rate constants were determined for each step.http://www.mdpi.com/1424-8220/8/8/4487/carbon dioxidemolecular biosensoroxidative stresshydrogen peroxide. |
| spellingShingle | Lech Chmurzyński Michał Woźniak Aleksandra Dąbrowska Agnieszka Chylewska Michał Szkatuła Dagmara Jacewicz Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity Sensors carbon dioxide molecular biosensor oxidative stress hydrogen peroxide. |
| title | Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity |
| title_full | Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity |
| title_fullStr | Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity |
| title_full_unstemmed | Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity |
| title_short | Coordinate cis-[Cr(C2O4)(pm)(OH2)2]+ Cation as Molecular Biosensor of Pyruvate’s Protective Activity Against Hydrogen Peroxide Mediated Cytotoxity |
| title_sort | coordinate cis cr c2o4 pm oh2 2 cation as molecular biosensor of pyruvatea¢a€a™s protective activity against hydrogen peroxide mediated cytotoxity |
| topic | carbon dioxide molecular biosensor oxidative stress hydrogen peroxide. |
| url | http://www.mdpi.com/1424-8220/8/8/4487/ |
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