Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids
For the production of polyhydroxyalkanoates (PHA) using nitrogen-rich feedstocks (e.g., protein-rich resources), the typical strategy of restricting cell growth as a means to enhance overall PHA productivity by nitrogen limitation is not applicable. In this case, a possible alternative to remove the...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/13/3/347 |
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| author | Kayo Santana Barros Mónica Carvalheira Bruno Costa Marreiros Maria Ascensão M. Reis João Goulão Crespo Valentín Pérez-Herranz Svetlozar Velizarov |
| author_facet | Kayo Santana Barros Mónica Carvalheira Bruno Costa Marreiros Maria Ascensão M. Reis João Goulão Crespo Valentín Pérez-Herranz Svetlozar Velizarov |
| author_sort | Kayo Santana Barros |
| collection | DOAJ |
| description | For the production of polyhydroxyalkanoates (PHA) using nitrogen-rich feedstocks (e.g., protein-rich resources), the typical strategy of restricting cell growth as a means to enhance overall PHA productivity by nitrogen limitation is not applicable. In this case, a possible alternative to remove the nitrogen excess (NH<sub>4</sub><sup>+</sup>/NH<sub>3</sub>) is by applying membrane separation processes. In the present study, the use of Donnan dialysis to separate ammonium ions from volatile fatty acids present in the media for the production of PHA was evaluated. Synthetic and real feed solutions were used, applying NaCl and HCl receiver solutions separated by commercial cation-exchange membranes. For this specific purpose, Fumasep and Ralex membranes showed better performance than Ionsep. Sorption of ammonium ions occurred in the Ralex membrane, thus intensifying the ammonium extraction. The separation performances with NaCl and HCl as receiver solutions were similar, despite sorption occurring in the Ralex membrane more intensely in the presence of NaCl. Higher volumetric flow rates, NaCl receiver concentrations, and volume ratios of feed:receiver solutions enhanced the degree of ammonium recovery. The application of an external electric potential difference to the two-compartment system did not significantly enhance the rate of ammonium appearance in the receiver solution. The results obtained using a real ammonium-containing solution after fermentation of cheese whey showed that Donnan dialysis can be successfully applied for ammonium recovery from such solutions. |
| first_indexed | 2024-03-11T06:11:16Z |
| format | Article |
| id | doaj.art-5541f6fbb257451da6d7a2318fdf56bf |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-11T06:11:16Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-5541f6fbb257451da6d7a2318fdf56bf2023-11-17T12:35:16ZengMDPI AGMembranes2077-03752023-03-0113334710.3390/membranes13030347Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty AcidsKayo Santana Barros0Mónica Carvalheira1Bruno Costa Marreiros2Maria Ascensão M. Reis3João Goulão Crespo4Valentín Pérez-Herranz5Svetlozar Velizarov6LAQV/REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalLAQV/REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalIEC Group, ISIRYM, Universitat Politècnica de València, Camí de Vera s/n, 46022, P.O. Box 22012, E-46071 València, SpainLAQV/REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalFor the production of polyhydroxyalkanoates (PHA) using nitrogen-rich feedstocks (e.g., protein-rich resources), the typical strategy of restricting cell growth as a means to enhance overall PHA productivity by nitrogen limitation is not applicable. In this case, a possible alternative to remove the nitrogen excess (NH<sub>4</sub><sup>+</sup>/NH<sub>3</sub>) is by applying membrane separation processes. In the present study, the use of Donnan dialysis to separate ammonium ions from volatile fatty acids present in the media for the production of PHA was evaluated. Synthetic and real feed solutions were used, applying NaCl and HCl receiver solutions separated by commercial cation-exchange membranes. For this specific purpose, Fumasep and Ralex membranes showed better performance than Ionsep. Sorption of ammonium ions occurred in the Ralex membrane, thus intensifying the ammonium extraction. The separation performances with NaCl and HCl as receiver solutions were similar, despite sorption occurring in the Ralex membrane more intensely in the presence of NaCl. Higher volumetric flow rates, NaCl receiver concentrations, and volume ratios of feed:receiver solutions enhanced the degree of ammonium recovery. The application of an external electric potential difference to the two-compartment system did not significantly enhance the rate of ammonium appearance in the receiver solution. The results obtained using a real ammonium-containing solution after fermentation of cheese whey showed that Donnan dialysis can be successfully applied for ammonium recovery from such solutions.https://www.mdpi.com/2077-0375/13/3/347Donnan dialysiscation-exchange membraneammonium sorptionammonium limitationbiopolymerprotein-rich feedstocks |
| spellingShingle | Kayo Santana Barros Mónica Carvalheira Bruno Costa Marreiros Maria Ascensão M. Reis João Goulão Crespo Valentín Pérez-Herranz Svetlozar Velizarov Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids Membranes Donnan dialysis cation-exchange membrane ammonium sorption ammonium limitation biopolymer protein-rich feedstocks |
| title | Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids |
| title_full | Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids |
| title_fullStr | Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids |
| title_full_unstemmed | Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids |
| title_short | Donnan Dialysis for Recovering Ammonium from Fermentation Solutions Rich in Volatile Fatty Acids |
| title_sort | donnan dialysis for recovering ammonium from fermentation solutions rich in volatile fatty acids |
| topic | Donnan dialysis cation-exchange membrane ammonium sorption ammonium limitation biopolymer protein-rich feedstocks |
| url | https://www.mdpi.com/2077-0375/13/3/347 |
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