Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells
H2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of brea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/11/1665 |
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| author | Christophe E. Redon Zoe Schmal Gargi Tewary Adèle Mangelinck Régis Courbeyrette Jean-Yves Thuret Mirit I. Aladjem William M. Bonner Claudia E. Rübe Carl Mann |
| author_facet | Christophe E. Redon Zoe Schmal Gargi Tewary Adèle Mangelinck Régis Courbeyrette Jean-Yves Thuret Mirit I. Aladjem William M. Bonner Claudia E. Rübe Carl Mann |
| author_sort | Christophe E. Redon |
| collection | DOAJ |
| description | H2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of breast, prostate, pancreas, thyroid, stomach, and salivary glands. H2A.J was also highly expressed in many carcinoma cell lines and in particular, those derived from luminal breast and prostate cancer. H2A.J thus appears to be a novel marker for luminal epithelial cancers. Knocking-out the <i>H2AFJ</i> gene in T47D luminal breast cancer cells reduced the expression of several estrogen-responsive genes which may explain its putative tumorigenic role in luminal-B breast cancer. |
| first_indexed | 2024-03-10T05:28:28Z |
| format | Article |
| id | doaj.art-55425fbdbbf947028af58aaa0d78030c |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T05:28:28Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-55425fbdbbf947028af58aaa0d78030c2023-11-22T23:26:59ZengMDPI AGGenes2073-44252021-10-011211166510.3390/genes12111665Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland CellsChristophe E. Redon0Zoe Schmal1Gargi Tewary2Adèle Mangelinck3Régis Courbeyrette4Jean-Yves Thuret5Mirit I. Aladjem6William M. Bonner7Claudia E. Rübe8Carl Mann9Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USADepartment of Radiation Oncology, Saarland University Hospital, 66421 Homburg/Saar, GermanyDepartment of Radiation Oncology, Saarland University Hospital, 66421 Homburg/Saar, GermanyInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91190 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91190 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91190 Gif-sur-Yvette, FranceDevelopmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USADevelopmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USADepartment of Radiation Oncology, Saarland University Hospital, 66421 Homburg/Saar, GermanyInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91190 Gif-sur-Yvette, FranceH2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of breast, prostate, pancreas, thyroid, stomach, and salivary glands. H2A.J was also highly expressed in many carcinoma cell lines and in particular, those derived from luminal breast and prostate cancer. H2A.J thus appears to be a novel marker for luminal epithelial cancers. Knocking-out the <i>H2AFJ</i> gene in T47D luminal breast cancer cells reduced the expression of several estrogen-responsive genes which may explain its putative tumorigenic role in luminal-B breast cancer.https://www.mdpi.com/2073-4425/12/11/1665histone variant H2A.Jluminal breast cancerluminal prostate cancercancer biomarker<i>H2AFJ</i>H2AJ |
| spellingShingle | Christophe E. Redon Zoe Schmal Gargi Tewary Adèle Mangelinck Régis Courbeyrette Jean-Yves Thuret Mirit I. Aladjem William M. Bonner Claudia E. Rübe Carl Mann Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells Genes histone variant H2A.J luminal breast cancer luminal prostate cancer cancer biomarker <i>H2AFJ</i> H2AJ |
| title | Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells |
| title_full | Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells |
| title_fullStr | Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells |
| title_full_unstemmed | Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells |
| title_short | Histone Variant H2A.J Is Enriched in Luminal Epithelial Gland Cells |
| title_sort | histone variant h2a j is enriched in luminal epithelial gland cells |
| topic | histone variant H2A.J luminal breast cancer luminal prostate cancer cancer biomarker <i>H2AFJ</i> H2AJ |
| url | https://www.mdpi.com/2073-4425/12/11/1665 |
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