Direct and Rapid Detection of Mycoplasma bovis in Bovine Milk Samples by Recombinase Polymerase Amplification Assays

Direct and Rapid Detection of Mycoplasma bovis in Bovine Milk Samples by Recombinase Polymerase Amplification Assays

This study aimed to detetct Mycoplasma bovis (M. bovis) in bovine milk quickly and directly by developing and validating isothermal recombinase polymerase amplification (RPA) assays. Targeting the uvrC gene of M. bovis, an RPA assay based on the fluorescence monitoring (real-time RPA) and an RPA ass...

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Bibliographic Details
Main Authors: Ruiwen Li, Jinfeng Wang, Xiaoxia Sun, Libing Liu, Jianchang Wang, Wanzhe Yuan
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Mycoplasma bovis
uvrC gene
real-time RPA
LFS RPA
isothermal amplification
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.639083/full

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