Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat
<i>Aragoa</i>, comprising 19 high-altitude North Andean species, is one of three genera in the Plantagineae (Plantaginaceae, Lamiales), along with <i>Littorella</i> and <i>Plantago</i>. Based primarily on plastid data and nuclear ITS, <i>Aragoa</i> is...
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MDPI AG
2021-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/12/2673 |
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| author | Jeffrey P. Mower Lilly Hanley Kirsten Wolff Natalia Pabón-Mora Favio González |
| author_facet | Jeffrey P. Mower Lilly Hanley Kirsten Wolff Natalia Pabón-Mora Favio González |
| author_sort | Jeffrey P. Mower |
| collection | DOAJ |
| description | <i>Aragoa</i>, comprising 19 high-altitude North Andean species, is one of three genera in the Plantagineae (Plantaginaceae, Lamiales), along with <i>Littorella</i> and <i>Plantago</i>. Based primarily on plastid data and nuclear ITS, <i>Aragoa</i> is sister to a clade of <i>Littorella</i> + <i>Plantago</i>, but Plantagineae relationships have yet to be assessed using multigene datasets from the nuclear and mitochondrial genomes. Here, complete mitogenomes were assembled for two species of <i>Aragoa</i> (<i>A. abietina</i> and <i>A. cleefii</i>). The mitogenomes of both species have a typical suite of genes for 34 proteins, 17 tRNAs, and three rRNAs. The <i>A. abietina</i> mitogenome assembled into a simple circular map, with no large repeats capable of producing alternative isoforms. The <i>A. cleefii</i> mitogenomic map was more complex, involving two circular maps bridged by a substoichiometric linear fragment. Phylogenetics of three mitochondrial genes or the nuclear rRNA repeat placed <i>Aragoa</i> as sister to <i>Littorella</i> + <i>Plantago</i>, consistent with previous studies. However, <i>P. nubicola</i>, the sole representative of subg. <i>Bougueria</i>, was nested within subg. <i>Psyllium</i> based on the mitochondrial and nuclear data, conflicting with plastid-based analyses. Phylogenetics of the nuclear rRNA repeat provided better resolution overall, whereas relationships from mitochondrial data were hindered by extensive substitution rate variation among lineages. |
| first_indexed | 2024-03-10T03:16:28Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:16:28Z |
| publishDate | 2021-12-01 |
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| series | Plants |
| spelling | doaj.art-554a28ed8a5049bda87a07a43e0052d92023-11-23T10:11:31ZengMDPI AGPlants2223-77472021-12-011012267310.3390/plants10122673Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA RepeatJeffrey P. Mower0Lilly Hanley1Kirsten Wolff2Natalia Pabón-Mora3Favio González4Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588, USACenter for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588, USASchool of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, UKInstituto de Biología, Universidad de Antioquia, Medellín 050010, ColombiaSede Bogotá, Facultad de Ciencias, Instituto de Ciencias Naturales, Universidad Nacional de Colombia, Bogotá 111321, Colombia<i>Aragoa</i>, comprising 19 high-altitude North Andean species, is one of three genera in the Plantagineae (Plantaginaceae, Lamiales), along with <i>Littorella</i> and <i>Plantago</i>. Based primarily on plastid data and nuclear ITS, <i>Aragoa</i> is sister to a clade of <i>Littorella</i> + <i>Plantago</i>, but Plantagineae relationships have yet to be assessed using multigene datasets from the nuclear and mitochondrial genomes. Here, complete mitogenomes were assembled for two species of <i>Aragoa</i> (<i>A. abietina</i> and <i>A. cleefii</i>). The mitogenomes of both species have a typical suite of genes for 34 proteins, 17 tRNAs, and three rRNAs. The <i>A. abietina</i> mitogenome assembled into a simple circular map, with no large repeats capable of producing alternative isoforms. The <i>A. cleefii</i> mitogenomic map was more complex, involving two circular maps bridged by a substoichiometric linear fragment. Phylogenetics of three mitochondrial genes or the nuclear rRNA repeat placed <i>Aragoa</i> as sister to <i>Littorella</i> + <i>Plantago</i>, consistent with previous studies. However, <i>P. nubicola</i>, the sole representative of subg. <i>Bougueria</i>, was nested within subg. <i>Psyllium</i> based on the mitochondrial and nuclear data, conflicting with plastid-based analyses. Phylogenetics of the nuclear rRNA repeat provided better resolution overall, whereas relationships from mitochondrial data were hindered by extensive substitution rate variation among lineages.https://www.mdpi.com/2223-7747/10/12/2673<i>Aragoa</i>mitochondrial genomenuclear rRNAphylogeneticsPlantagineae |
| spellingShingle | Jeffrey P. Mower Lilly Hanley Kirsten Wolff Natalia Pabón-Mora Favio González Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat Plants <i>Aragoa</i> mitochondrial genome nuclear rRNA phylogenetics Plantagineae |
| title | Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat |
| title_full | Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat |
| title_fullStr | Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat |
| title_full_unstemmed | Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat |
| title_short | Complete Mitogenomes of Two <i>Aragoa</i> Species and Phylogeny of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes and the Nuclear Ribosomal RNA Repeat |
| title_sort | complete mitogenomes of two i aragoa i species and phylogeny of plantagineae plantaginaceae lamiales using mitochondrial genes and the nuclear ribosomal rna repeat |
| topic | <i>Aragoa</i> mitochondrial genome nuclear rRNA phylogenetics Plantagineae |
| url | https://www.mdpi.com/2223-7747/10/12/2673 |
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