Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste
Industrial waste from Jumputan cloth production pose a significant risk to environmental safety due to their toxic synthetic dye content. Several studies have shown that the presence of bacteria in these materials plays a very important role in decolorization process of the constituent dye. Therefor...
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| Format: | Article |
| Language: | English |
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Magister Program of Material Sciences, Graduate School of Universitas Sriwijaya
2023-07-01
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| Series: | Science and Technology Indonesia |
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| Online Access: | https://sciencetechindonesia.com/index.php/jsti/article/view/733/334 |
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| author | Muharni Elisa Nurnawati Heni Yohandini Hary Widjajanti |
| author_facet | Muharni Elisa Nurnawati Heni Yohandini Hary Widjajanti |
| author_sort | Muharni |
| collection | DOAJ |
| description | Industrial waste from Jumputan cloth production pose a significant risk to environmental safety due to their toxic synthetic dye content. Several studies have shown that the presence of bacteria in these materials plays a very important role in decolorization process of the constituent dye. Therefore, this study aims to isolate bacteria with the ability to decolorize direct red 80 from Jumputan cloth industrial waste. Characterization of isolates was carried out macroscopically, microscopically, and biochemically, followed by molecular identification using the 16S rRNA gene. Decolorization effects of the samples on red dye 80 were then assessed using a spectrophotometer at a maximum wavelength of 528 nm. The results showed that 6 bacteria isolates can degrade dye, with decolorizing power ranging from 26.33±0.94 - 73.67±0.47. The highest potential for decolorizing waste synthetic dye is seen in isolate BD 05. Phylogenetic analysis showed that there were 3 genera of bacteria among the samples obtained, namely Bacillus, Aeromonas, and Pseudomonas. These bacteria were closely related to Bacillus tropicus, Areomonas jandaei, and Pseudomonas stutzeri. Pseudomonas stutzeri (BD 05) has the highest potential in handling jumputan industrial waste. |
| first_indexed | 2024-03-13T00:55:06Z |
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| id | doaj.art-554a4246fb1f440cafea2017f164dceb |
| institution | Directory Open Access Journal |
| issn | 2580-4405 2580-4391 |
| language | English |
| last_indexed | 2024-04-24T14:08:37Z |
| publishDate | 2023-07-01 |
| publisher | Magister Program of Material Sciences, Graduate School of Universitas Sriwijaya |
| record_format | Article |
| series | Science and Technology Indonesia |
| spelling | doaj.art-554a4246fb1f440cafea2017f164dceb2024-04-03T09:42:58ZengMagister Program of Material Sciences, Graduate School of Universitas SriwijayaScience and Technology Indonesia2580-44052580-43912023-07-018342943510.26554/sti.2023.8.3.429-435Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial WasteMuharni0Elisa Nurnawati1Heni Yohandini2Hary Widjajanti3Department of Biology, FMIPA Sriwijaya University, Indralaya, 30662, IndonesiaDepartment of Biology, FMIPA Sriwijaya University, Indralaya, 30662, IndonesiaDepartment of Chemistry, FMIPA Sriwijaya University, Indralaya, 30662, IndonesiaDepartment of Biology, FMIPA Sriwijaya University, Indralaya, 30662, IndonesiaIndustrial waste from Jumputan cloth production pose a significant risk to environmental safety due to their toxic synthetic dye content. Several studies have shown that the presence of bacteria in these materials plays a very important role in decolorization process of the constituent dye. Therefore, this study aims to isolate bacteria with the ability to decolorize direct red 80 from Jumputan cloth industrial waste. Characterization of isolates was carried out macroscopically, microscopically, and biochemically, followed by molecular identification using the 16S rRNA gene. Decolorization effects of the samples on red dye 80 were then assessed using a spectrophotometer at a maximum wavelength of 528 nm. The results showed that 6 bacteria isolates can degrade dye, with decolorizing power ranging from 26.33±0.94 - 73.67±0.47. The highest potential for decolorizing waste synthetic dye is seen in isolate BD 05. Phylogenetic analysis showed that there were 3 genera of bacteria among the samples obtained, namely Bacillus, Aeromonas, and Pseudomonas. These bacteria were closely related to Bacillus tropicus, Areomonas jandaei, and Pseudomonas stutzeri. Pseudomonas stutzeri (BD 05) has the highest potential in handling jumputan industrial waste.https://sciencetechindonesia.com/index.php/jsti/article/view/733/334decolorizationdirect red 8016s rrna gene |
| spellingShingle | Muharni Elisa Nurnawati Heni Yohandini Hary Widjajanti Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste Science and Technology Indonesia decolorization direct red 80 16s rrna gene |
| title | Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste |
| title_full | Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste |
| title_fullStr | Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste |
| title_full_unstemmed | Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste |
| title_short | Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste |
| title_sort | isolation and molecular identification of direct red 80 synthetic dye degradation bacteria from palembang indonesia jumputan cloth industrial waste |
| topic | decolorization direct red 80 16s rrna gene |
| url | https://sciencetechindonesia.com/index.php/jsti/article/view/733/334 |
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