Heterofucans from Dictyota menstrualis have anticoagulant activity
Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the struc...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2004-02-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200002 |
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| author | I.R.L. Albuquerque K.C.S. Queiroz L.G. Alves E.A. Santos E.L. Leite H.A.O. Rocha |
| author_facet | I.R.L. Albuquerque K.C.S. Queiroz L.G. Alves E.A. Santos E.L. Leite H.A.O. Rocha |
| author_sort | I.R.L. Albuquerque |
| collection | DOAJ |
| description | Fucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all fractions are composed mainly of fucose, xylose, galactose, uronic acid, and sulfate. The anticoagulant activity of these heterofucans was determined by activated partial thromboplastin time (APTT) using citrate normal human plasma. Only the fucans F1.0v and F1.5v showed anticoagulant activity. To prolong the coagulation time to double the baseline value in the APTT, the required concentration of fucan F1.0v (20 µg/ml) was only 4.88-fold higher than that of the low molecular weight heparin Clexane® (4.1 µg/ml), whereas 80 µg/ml fucan 1.5 was needed to obtain the same effect. For both fucans this effect was abolished by desulfation. These polymers are composed of fucose, xylose, uronic acid, galactose, and sulfate at molar ratios of 1.0:0.8:0.7:0.8:0.4 and 1.0:0.3:0.4:1.5:1.3, respectively. This is the fist report indicating the presence of a heterofucan with higher anticoagulant activity from brown seaweed. |
| first_indexed | 2024-04-13T18:19:44Z |
| format | Article |
| id | doaj.art-554b28276e7f4f2884a6fb231fc615c1 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-13T18:19:44Z |
| publishDate | 2004-02-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-554b28276e7f4f2884a6fb231fc615c12022-12-22T02:35:29ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2004-02-0137216717110.1590/S0100-879X2004000200002Heterofucans from Dictyota menstrualis have anticoagulant activityI.R.L. AlbuquerqueK.C.S. QueirozL.G. AlvesE.A. SantosE.L. LeiteH.A.O. RochaFucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all fractions are composed mainly of fucose, xylose, galactose, uronic acid, and sulfate. The anticoagulant activity of these heterofucans was determined by activated partial thromboplastin time (APTT) using citrate normal human plasma. Only the fucans F1.0v and F1.5v showed anticoagulant activity. To prolong the coagulation time to double the baseline value in the APTT, the required concentration of fucan F1.0v (20 µg/ml) was only 4.88-fold higher than that of the low molecular weight heparin Clexane® (4.1 µg/ml), whereas 80 µg/ml fucan 1.5 was needed to obtain the same effect. For both fucans this effect was abolished by desulfation. These polymers are composed of fucose, xylose, uronic acid, galactose, and sulfate at molar ratios of 1.0:0.8:0.7:0.8:0.4 and 1.0:0.3:0.4:1.5:1.3, respectively. This is the fist report indicating the presence of a heterofucan with higher anticoagulant activity from brown seaweed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200002FucanFucoidanAnticoagulant drugsBrown algaeAntithrombotic agents |
| spellingShingle | I.R.L. Albuquerque K.C.S. Queiroz L.G. Alves E.A. Santos E.L. Leite H.A.O. Rocha Heterofucans from Dictyota menstrualis have anticoagulant activity Brazilian Journal of Medical and Biological Research Fucan Fucoidan Anticoagulant drugs Brown algae Antithrombotic agents |
| title | Heterofucans from Dictyota menstrualis have anticoagulant activity |
| title_full | Heterofucans from Dictyota menstrualis have anticoagulant activity |
| title_fullStr | Heterofucans from Dictyota menstrualis have anticoagulant activity |
| title_full_unstemmed | Heterofucans from Dictyota menstrualis have anticoagulant activity |
| title_short | Heterofucans from Dictyota menstrualis have anticoagulant activity |
| title_sort | heterofucans from dictyota menstrualis have anticoagulant activity |
| topic | Fucan Fucoidan Anticoagulant drugs Brown algae Antithrombotic agents |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200002 |
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