Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it>
<p>Abstract</p> <p>Background</p> <p><it>Ralstonia pickettii </it>is a nosocomial infectious agent and a significant industrial contaminant. It has been found in many different environments including clinical situations, soil and industrial High Purity Water...
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| Format: | Article |
| Language: | English |
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BMC
2011-08-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/11/194 |
| _version_ | 1818352964317741056 |
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| author | Adley Catherine C Pembroke J Tony Ryan Michael P |
| author_facet | Adley Catherine C Pembroke J Tony Ryan Michael P |
| author_sort | Adley Catherine C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Ralstonia pickettii </it>is a nosocomial infectious agent and a significant industrial contaminant. It has been found in many different environments including clinical situations, soil and industrial High Purity Water. This study compares the phenotypic and genotypic diversity of a selection of strains of <it>Ralstonia </it>collected from a variety of sources.</p> <p>Results</p> <p><it>Ralstonia </it>isolates (fifty-nine) from clinical, industrial and environmental origins were compared genotypically using i) Species-specific-PCR, ii) PCR and sequencing of the 16<it>S-</it>23<it>S </it>rRNA Interspatial region (ISR) iii) the <it>fliC </it>gene genes, iv) RAPD and BOX-PCR and v) phenotypically using biochemical testing. The species specific-PCR identified fifteen out of fifty-nine designated <it>R. pickettii </it>isolates as actually being the closely related species <it>R. insidiosa</it>. PCR-ribotyping of the 16<it>S-</it>23<it>S </it>rRNA ISR indicated few major differences between the isolates. Analysis of all isolates demonstrated different banding patterns for both the RAPD and BOX primers however these were found not to vary significantly.</p> <p>Conclusions</p> <p><it>R. pickettii </it>species isolated from wide geographic and environmental sources appear to be reasonably homogenous based on genotypic and phenotypic characteristics. <it>R. insidiosa </it>can at present only be distinguished from <it>R. pickettii </it>using species specific PCR. <it>R. pickettii </it>and <it>R. insidiosa </it>isolates do not differ significantly phenotypically or genotypically based on environmental or geographical origin.</p> |
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| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-13T19:02:00Z |
| publishDate | 2011-08-01 |
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| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-554c59164b0541229ed6f4d9e33b41f82022-12-21T23:34:40ZengBMCBMC Microbiology1471-21802011-08-0111119410.1186/1471-2180-11-194Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it>Adley Catherine CPembroke J TonyRyan Michael P<p>Abstract</p> <p>Background</p> <p><it>Ralstonia pickettii </it>is a nosocomial infectious agent and a significant industrial contaminant. It has been found in many different environments including clinical situations, soil and industrial High Purity Water. This study compares the phenotypic and genotypic diversity of a selection of strains of <it>Ralstonia </it>collected from a variety of sources.</p> <p>Results</p> <p><it>Ralstonia </it>isolates (fifty-nine) from clinical, industrial and environmental origins were compared genotypically using i) Species-specific-PCR, ii) PCR and sequencing of the 16<it>S-</it>23<it>S </it>rRNA Interspatial region (ISR) iii) the <it>fliC </it>gene genes, iv) RAPD and BOX-PCR and v) phenotypically using biochemical testing. The species specific-PCR identified fifteen out of fifty-nine designated <it>R. pickettii </it>isolates as actually being the closely related species <it>R. insidiosa</it>. PCR-ribotyping of the 16<it>S-</it>23<it>S </it>rRNA ISR indicated few major differences between the isolates. Analysis of all isolates demonstrated different banding patterns for both the RAPD and BOX primers however these were found not to vary significantly.</p> <p>Conclusions</p> <p><it>R. pickettii </it>species isolated from wide geographic and environmental sources appear to be reasonably homogenous based on genotypic and phenotypic characteristics. <it>R. insidiosa </it>can at present only be distinguished from <it>R. pickettii </it>using species specific PCR. <it>R. pickettii </it>and <it>R. insidiosa </it>isolates do not differ significantly phenotypically or genotypically based on environmental or geographical origin.</p>http://www.biomedcentral.com/1471-2180/11/194<it>Ralstonia pickettii</it>random amplified polymorphic DNAPCRgenotypingHigh-Purity Water |
| spellingShingle | Adley Catherine C Pembroke J Tony Ryan Michael P Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it> BMC Microbiology <it>Ralstonia pickettii</it> random amplified polymorphic DNA PCR genotyping High-Purity Water |
| title | Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it> |
| title_full | Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it> |
| title_fullStr | Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it> |
| title_full_unstemmed | Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it> |
| title_short | Genotypic and phenotypic diversity of <it>Ralstonia pickettii </it>and <it>Ralstonia insidiosa </it>isolates from clinical and environmental sources including High-purity Water. Diversity in <it>Ralstonia pickettii</it> |
| title_sort | genotypic and phenotypic diversity of it ralstonia pickettii it and it ralstonia insidiosa it isolates from clinical and environmental sources including high purity water diversity in it ralstonia pickettii it |
| topic | <it>Ralstonia pickettii</it> random amplified polymorphic DNA PCR genotyping High-Purity Water |
| url | http://www.biomedcentral.com/1471-2180/11/194 |
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