Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions
Simple protein elastic networks which neglect amino-acid information often yield reasonable predictions of conformational dynamics and are broadly used. Recently, model variants which incorporate sequence-specific and distance-dependent interactions of residue pairs have been constructed and demonst...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/10/549 |
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| author | Romain Amyot Yuichi Togashi Holger Flechsig |
| author_facet | Romain Amyot Yuichi Togashi Holger Flechsig |
| author_sort | Romain Amyot |
| collection | DOAJ |
| description | Simple protein elastic networks which neglect amino-acid information often yield reasonable predictions of conformational dynamics and are broadly used. Recently, model variants which incorporate sequence-specific and distance-dependent interactions of residue pairs have been constructed and demonstrated to improve agreement with experimental data. We have applied the new variants in a systematic study of protein fluctuation properties and compared their predictions with those of conventional anisotropic network models. We find that the quality of predictions is frequently linked to poor estimations in highly flexible protein regions. An analysis of a large set of protein structures shows that fluctuations of very weakly connected network residues are intrinsically prone to be significantly overestimated by all models. This problem persists in the new models and is not resolved by taking into account sequence information. The effect becomes even enhanced in the model variant which takes into account very soft long-ranged residue interactions. Beyond these shortcomings, we find that model predictions are largely insensitive to the integration of chemical information, at least regarding the fluctuation properties of individual residues. One can furthermore conclude that the inherent drawbacks may present a serious hindrance when improvement of elastic network models are attempted. |
| first_indexed | 2024-12-20T08:37:55Z |
| format | Article |
| id | doaj.art-554ddb88453540e1bc2705bcd09a5bdb |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-20T08:37:55Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj.art-554ddb88453540e1bc2705bcd09a5bdb2022-12-21T19:46:29ZengMDPI AGBiomolecules2218-273X2019-09-0191054910.3390/biom9100549biom9100549Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent InteractionsRomain Amyot0Yuichi Togashi1Holger Flechsig2Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, JapanResearch Center for the Mathematics on Chromatin Live Dynamics (RcMcD), Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, JapanNano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, JapanSimple protein elastic networks which neglect amino-acid information often yield reasonable predictions of conformational dynamics and are broadly used. Recently, model variants which incorporate sequence-specific and distance-dependent interactions of residue pairs have been constructed and demonstrated to improve agreement with experimental data. We have applied the new variants in a systematic study of protein fluctuation properties and compared their predictions with those of conventional anisotropic network models. We find that the quality of predictions is frequently linked to poor estimations in highly flexible protein regions. An analysis of a large set of protein structures shows that fluctuations of very weakly connected network residues are intrinsically prone to be significantly overestimated by all models. This problem persists in the new models and is not resolved by taking into account sequence information. The effect becomes even enhanced in the model variant which takes into account very soft long-ranged residue interactions. Beyond these shortcomings, we find that model predictions are largely insensitive to the integration of chemical information, at least regarding the fluctuation properties of individual residues. One can furthermore conclude that the inherent drawbacks may present a serious hindrance when improvement of elastic network models are attempted.https://www.mdpi.com/2218-273X/9/10/549protein fluctuationscoarse-grained modelingelastic networksb-factorssequence specificity |
| spellingShingle | Romain Amyot Yuichi Togashi Holger Flechsig Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions Biomolecules protein fluctuations coarse-grained modeling elastic networks b-factors sequence specificity |
| title | Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions |
| title_full | Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions |
| title_fullStr | Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions |
| title_full_unstemmed | Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions |
| title_short | Analyzing Fluctuation Properties in Protein Elastic Networks with Sequence-Specific and Distance-Dependent Interactions |
| title_sort | analyzing fluctuation properties in protein elastic networks with sequence specific and distance dependent interactions |
| topic | protein fluctuations coarse-grained modeling elastic networks b-factors sequence specificity |
| url | https://www.mdpi.com/2218-273X/9/10/549 |
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