Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed
Abstract We aim to assess the antibacterial and anti-biofilm properties of Niosome-encapsulated Imipenem. After isolating Staphylococcus epidermidis isolates and determining their microbial sensitivity, their ability to form biofilms was examined using plate microtiter assay. Various formulations of...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09195-9 |
| _version_ | 1818772515510550528 |
|---|---|
| author | Tohid Piri-Gharaghie Neda Jegargoshe-Shirin Sara Saremi-Nouri Seyed-hossein Khademhosseini Eskandar Hoseinnezhad-lazarjani Aezam Mousavi Hamidreza Kabiri Negin Rajaei Anali Riahi Ali Farhadi-Biregani Sadegh Fatehi-Ghahfarokhi |
| author_facet | Tohid Piri-Gharaghie Neda Jegargoshe-Shirin Sara Saremi-Nouri Seyed-hossein Khademhosseini Eskandar Hoseinnezhad-lazarjani Aezam Mousavi Hamidreza Kabiri Negin Rajaei Anali Riahi Ali Farhadi-Biregani Sadegh Fatehi-Ghahfarokhi |
| author_sort | Tohid Piri-Gharaghie |
| collection | DOAJ |
| description | Abstract We aim to assess the antibacterial and anti-biofilm properties of Niosome-encapsulated Imipenem. After isolating Staphylococcus epidermidis isolates and determining their microbial sensitivity, their ability to form biofilms was examined using plate microtiter assay. Various formulations of Niosome-encapsulated Imipenem were prepared using the thin-film hydration method, Minimum Biofilm Inhibitory Concentration (MBIC) and Minimum Inhibitory Concentration (MIC) were determined, and biofilm genes expression was examined. Drug formulations’ toxicity effect on HDF cells were determined using MTT assay. Out of the 162 separated S. epidermidis, 106 were resistant to methicillin. 87 MRSE isolates were vancomycin-resistant, all of which could form biofilms. The F1 formulation of niosomal Imipenem with a size of 192.3 ± 5.84 and an encapsulation index of 79.36 ± 1.14 was detected, which prevented biofilm growth with a BGI index of 69% and reduced icaD, FnbA, EbpS biofilms’ expression with P ≤ 0.001 in addition to reducing MBIC and MIC by 4–6 times. Interestingly, F1 formulation of niosomal Imipenem indicated cell viability over 90% at all tested concentrations. The results of the present study indicate that Niosome-encapsulated Imipenem reduces the resistance of MRSE to antibiotics in addition to increasing its anti-biofilm and antibiotic activity, and could prove useful as a new strategy for drug delivery. |
| first_indexed | 2024-12-18T10:10:35Z |
| format | Article |
| id | doaj.art-554e3af32afa4ec5af217e4f218f5a55 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T10:10:35Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-554e3af32afa4ec5af217e4f218f5a552022-12-21T21:11:26ZengNature PortfolioScientific Reports2045-23222022-03-0112111310.1038/s41598-022-09195-9Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formedTohid Piri-Gharaghie0Neda Jegargoshe-Shirin1Sara Saremi-Nouri2Seyed-hossein Khademhosseini3Eskandar Hoseinnezhad-lazarjani4Aezam Mousavi5Hamidreza Kabiri6Negin Rajaei7Anali Riahi8Ali Farhadi-Biregani9Sadegh Fatehi-Ghahfarokhi10Biotechnology Research Center, Microbial Biotechnology Laboratory, AmitisGen Med TECH GroupDepartment of Biotechnology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad UniversityDepartment of Biology, Faculty of Basic Sciences, Azarbaijan Branch, Azarbaijan Shahid Madani UniversityBiotechnology Research Center, Islamic Azad University, Tonekabon BranchBiotechnology Research Center, Islamic Azad University, Shahrekord BranchBiotechnology Research Center, Islamic Azad University, Shahrekord BranchYoung Researchers and Elite Club, Shahrekord Branch, Islamic Azad UniversityYoung Researchers and Elite Club, Shahrekord Branch, Islamic Azad UniversityDepartment of Biotechnology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad UniversityDepartment of Biotechnology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad UniversityDepartment of Biotechnology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad UniversityAbstract We aim to assess the antibacterial and anti-biofilm properties of Niosome-encapsulated Imipenem. After isolating Staphylococcus epidermidis isolates and determining their microbial sensitivity, their ability to form biofilms was examined using plate microtiter assay. Various formulations of Niosome-encapsulated Imipenem were prepared using the thin-film hydration method, Minimum Biofilm Inhibitory Concentration (MBIC) and Minimum Inhibitory Concentration (MIC) were determined, and biofilm genes expression was examined. Drug formulations’ toxicity effect on HDF cells were determined using MTT assay. Out of the 162 separated S. epidermidis, 106 were resistant to methicillin. 87 MRSE isolates were vancomycin-resistant, all of which could form biofilms. The F1 formulation of niosomal Imipenem with a size of 192.3 ± 5.84 and an encapsulation index of 79.36 ± 1.14 was detected, which prevented biofilm growth with a BGI index of 69% and reduced icaD, FnbA, EbpS biofilms’ expression with P ≤ 0.001 in addition to reducing MBIC and MIC by 4–6 times. Interestingly, F1 formulation of niosomal Imipenem indicated cell viability over 90% at all tested concentrations. The results of the present study indicate that Niosome-encapsulated Imipenem reduces the resistance of MRSE to antibiotics in addition to increasing its anti-biofilm and antibiotic activity, and could prove useful as a new strategy for drug delivery.https://doi.org/10.1038/s41598-022-09195-9 |
| spellingShingle | Tohid Piri-Gharaghie Neda Jegargoshe-Shirin Sara Saremi-Nouri Seyed-hossein Khademhosseini Eskandar Hoseinnezhad-lazarjani Aezam Mousavi Hamidreza Kabiri Negin Rajaei Anali Riahi Ali Farhadi-Biregani Sadegh Fatehi-Ghahfarokhi Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed Scientific Reports |
| title | Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed |
| title_full | Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed |
| title_fullStr | Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed |
| title_full_unstemmed | Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed |
| title_short | Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed |
| title_sort | effects of imipenem containing niosome nanoparticles against high prevalence methicillin resistant staphylococcus epidermidis biofilm formed |
| url | https://doi.org/10.1038/s41598-022-09195-9 |
| work_keys_str_mv | AT tohidpirigharaghie effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT nedajegargosheshirin effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT sarasareminouri effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT seyedhosseinkhademhosseini effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT eskandarhoseinnezhadlazarjani effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT aezammousavi effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT hamidrezakabiri effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT neginrajaei effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT analiriahi effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT alifarhadibiregani effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed AT sadeghfatehighahfarokhi effectsofimipenemcontainingniosomenanoparticlesagainsthighprevalencemethicillinresistantstaphylococcusepidermidisbiofilmformed |