Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens
Aim: Green synthesis of silver nanoparticles from medicinal plants have been progressively acquiring attractiveness to the researchers due to its sustainable nature, nontoxic and economically beneficial. The present study was to synthesize silver nanoparticles (AgNPs) from aqueous extract of Commeli...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722005547 |
| _version_ | 1811295468312854528 |
|---|---|
| author | R. Vijay V.M. Drisya Daniel Raja Femil Selta M.A. Rathi VK. Gopalakrishnan Dalal Hussien M. Alkhalifah Wael N. Hozzein |
| author_facet | R. Vijay V.M. Drisya Daniel Raja Femil Selta M.A. Rathi VK. Gopalakrishnan Dalal Hussien M. Alkhalifah Wael N. Hozzein |
| author_sort | R. Vijay |
| collection | DOAJ |
| description | Aim: Green synthesis of silver nanoparticles from medicinal plants have been progressively acquiring attractiveness to the researchers due to its sustainable nature, nontoxic and economically beneficial. The present study was to synthesize silver nanoparticles (AgNPs) from aqueous extract of Commelina forskaolii Vahl and exhibit its potential antimicrobial and cytotoxic activity. Material and Methods: The whole plant of Commelina forskaolii was used to synthesize AgNPs. The synthesized AgNPs was then characterized by UV – visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The AgNps are widely tested for antibacterial, antifungal and cytotoxic property. Results: The phytochemical screening of the aqueous extract showed the presence of secondary metabolites such as alkaloids, flavonoids, tannins, phenols, saponins, steroids, glycosides and proteins. The UV – vis absorption spectrum exhibited key peaks at 425 nm. FTIR spectrum revealed that the biochemical compounds are responsible for the reduction and capping material of AgNPs. SEM analysis showed, the average size of synthesized AgNPs ranged from 18 to 27 nm. TEM micrographs revealed that the particle size was to be 30–40 nm. The AgNPs exhibited potential antimicrobial activity against bacterial species (Enterococcus fecalis, Pseudomonas aeruginosa) showed MIC at about 62.5 µg/ml and 125 µg/ml respectively and fungal species (Candida albicans and Aspergilus niger) 250 µg/ml and 31.2 µg/ml respectively. The synthesized AgNPs showed potential cytotoxic activity against human breast cancer cell line (MCF-7) with the IC50 value of 50.2 µg/ml. The present investigation concludes the effectiveness of confirmed AgNPs might be used in pharmacological field for the treatment of bacterial, fungal and breast cancer. |
| first_indexed | 2024-04-13T05:33:51Z |
| format | Article |
| id | doaj.art-554e9b5fd0754b2795f29381650c021d |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-13T05:33:51Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-554e9b5fd0754b2795f29381650c021d2022-12-22T03:00:21ZengElsevierJournal of King Saud University: Science1018-36472023-01-01351102373Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogensR. Vijay0V.M. Drisya1Daniel Raja Femil Selta2M.A. Rathi3VK. Gopalakrishnan4Dalal Hussien M. Alkhalifah5Wael N. Hozzein6Department of Biochemistry, Sree Narayana Guru College, Coimbatore – 641 105, TamilNadu, IndiaDepartment of Biochemistry, Sree Narayana Guru College, Coimbatore – 641 105, TamilNadu, IndiaDepartment of Biochemistry and Cancer Research Center, FASCM, Karpagam Academy of Higher Education, Coimbatore 641 021, Tamil Nadu, IndiaDepartment of Biochemistry and Cancer Research Center, FASCM, Karpagam Academy of Higher Education, Coimbatore 641 021, Tamil Nadu, India; Corresponding authors.School of Medicine, Bule Hora University Institute of Health, Bule Hora University, BuleHora, Ethiopia; Corresponding authors.Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, B.O. Box 84428, Riyadh 11671, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptAim: Green synthesis of silver nanoparticles from medicinal plants have been progressively acquiring attractiveness to the researchers due to its sustainable nature, nontoxic and economically beneficial. The present study was to synthesize silver nanoparticles (AgNPs) from aqueous extract of Commelina forskaolii Vahl and exhibit its potential antimicrobial and cytotoxic activity. Material and Methods: The whole plant of Commelina forskaolii was used to synthesize AgNPs. The synthesized AgNPs was then characterized by UV – visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The AgNps are widely tested for antibacterial, antifungal and cytotoxic property. Results: The phytochemical screening of the aqueous extract showed the presence of secondary metabolites such as alkaloids, flavonoids, tannins, phenols, saponins, steroids, glycosides and proteins. The UV – vis absorption spectrum exhibited key peaks at 425 nm. FTIR spectrum revealed that the biochemical compounds are responsible for the reduction and capping material of AgNPs. SEM analysis showed, the average size of synthesized AgNPs ranged from 18 to 27 nm. TEM micrographs revealed that the particle size was to be 30–40 nm. The AgNPs exhibited potential antimicrobial activity against bacterial species (Enterococcus fecalis, Pseudomonas aeruginosa) showed MIC at about 62.5 µg/ml and 125 µg/ml respectively and fungal species (Candida albicans and Aspergilus niger) 250 µg/ml and 31.2 µg/ml respectively. The synthesized AgNPs showed potential cytotoxic activity against human breast cancer cell line (MCF-7) with the IC50 value of 50.2 µg/ml. The present investigation concludes the effectiveness of confirmed AgNPs might be used in pharmacological field for the treatment of bacterial, fungal and breast cancer.http://www.sciencedirect.com/science/article/pii/S1018364722005547Commelina forskaoliiMTTTEMSilver nanoparticlesAntimicrobialCytotoxic activity |
| spellingShingle | R. Vijay V.M. Drisya Daniel Raja Femil Selta M.A. Rathi VK. Gopalakrishnan Dalal Hussien M. Alkhalifah Wael N. Hozzein Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens Journal of King Saud University: Science Commelina forskaolii MTT TEM Silver nanoparticles Antimicrobial Cytotoxic activity |
| title | Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens |
| title_full | Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens |
| title_fullStr | Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens |
| title_full_unstemmed | Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens |
| title_short | Synthesis and characterization of silver nanomaterial from aqueous extract of Commelina forskaolii and its potential antimicrobial activity against Gram negative pathogens |
| title_sort | synthesis and characterization of silver nanomaterial from aqueous extract of commelina forskaolii and its potential antimicrobial activity against gram negative pathogens |
| topic | Commelina forskaolii MTT TEM Silver nanoparticles Antimicrobial Cytotoxic activity |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722005547 |
| work_keys_str_mv | AT rvijay synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens AT vmdrisya synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens AT danielrajafemilselta synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens AT marathi synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens AT vkgopalakrishnan synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens AT dalalhussienmalkhalifah synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens AT waelnhozzein synthesisandcharacterizationofsilvernanomaterialfromaqueousextractofcommelinaforskaoliianditspotentialantimicrobialactivityagainstgramnegativepathogens |