Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae)
Infections by multiresistant microorganisms have led to a continuous investigation of substances acting as modifiers of this resistance. By following this approach, the chemical composition of the essential oil from <i>Piper caldense</i> leaf and its antimicrobial potential were investig...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/2/631 |
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| author | José Weverton Almeida Bezerra Felicidade Caroline Rodrigues Rafael Pereira da Cruz Luiz Everson da Silva Wanderlei do Amaral Ricardo Andrade Rebelo Ieda Maria Begnini Camila Fonseca Bezerra Marcello Iriti Elena Maria Varoni Henrique Douglas Melo Coutinho Maria Flaviana Bezerra Morais-Braga |
| author_facet | José Weverton Almeida Bezerra Felicidade Caroline Rodrigues Rafael Pereira da Cruz Luiz Everson da Silva Wanderlei do Amaral Ricardo Andrade Rebelo Ieda Maria Begnini Camila Fonseca Bezerra Marcello Iriti Elena Maria Varoni Henrique Douglas Melo Coutinho Maria Flaviana Bezerra Morais-Braga |
| author_sort | José Weverton Almeida Bezerra |
| collection | DOAJ |
| description | Infections by multiresistant microorganisms have led to a continuous investigation of substances acting as modifiers of this resistance. By following this approach, the chemical composition of the essential oil from <i>Piper caldense</i> leaf and its antimicrobial potential were investigated. The antimicrobial activity was determined by broth microdilution method providing values for minimum inhibitory concentration (MIC), IC<sub>50</sub>, and minimum fungicidal concentration (MFC). The essential oil was tested as a modulator for several antibiotics, and its effect on the morphology of <i>Candida</i> <i>albicans</i> (CA) strains was also investigated. The chemical characterization revealed an oil composed mainly of sesquiterpenes. Among them are caryophyllene oxide (13.9%), spathulenol (9.1%), δ-cadinene (7.6%) and bicyclogermacrene (6.7%) with the highest concentrations. The essential oil showed very low activity against the strains of CA with the lowest values for IC<sub>50</sub> and MFC of 1790 μg/mL and 8192 μg/mL, respectively. The essential oil modulated the activity of fluconazole against CA URM 4387 strain, which was demonstrated by the lower IC<sub>50</sub> obtained, 2.7 μg/mL, whereas fluconazole itself presented an IC<sub>50</sub> of 7.76 μg/mL. No modulating effect was observed in the MFC bioassays. The effect on fungal morphology was observed for both CA INCQS 40006 and URM 4387 strains. The hyphae projection was completely inhibited at 4096 μg/mL and 2048 μg/mL, respectively. Thus, the oil has potential as an adjuvant in antimicrobial formulations. |
| first_indexed | 2024-12-16T07:00:20Z |
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| id | doaj.art-554fe07b10d24157a31a7a94ba818cf0 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-16T07:00:20Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-554fe07b10d24157a31a7a94ba818cf02022-12-21T22:40:11ZengMDPI AGApplied Sciences2076-34172020-01-0110263110.3390/app10020631app10020631Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae)José Weverton Almeida Bezerra0Felicidade Caroline Rodrigues1Rafael Pereira da Cruz2Luiz Everson da Silva3Wanderlei do Amaral4Ricardo Andrade Rebelo5Ieda Maria Begnini6Camila Fonseca Bezerra7Marcello Iriti8Elena Maria Varoni9Henrique Douglas Melo Coutinho10Maria Flaviana Bezerra Morais-Braga11Postgraduate Program in Plant Biology, Federal University of Pernambuco—UFPE, Recife 50670-901, PE, BrazilPostgraduate Program in Plant Biology, Federal University of Pernambuco—UFPE, Recife 50670-901, PE, BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilResearch Laboratory of Natural Products, Federal University of Paraná—UFPR, Matinhos 83260-000, PR, BrazilResearch Laboratory of Natural Products, Federal University of Paraná—UFPR, Matinhos 83260-000, PR, BrazilResearch Laboratory of Natural Products, Federal University of Paraná—UFPR, Matinhos 83260-000, PR, BrazilResearch Laboratory of Natural Products, Federal University of Paraná—UFPR, Matinhos 83260-000, PR, BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilDepartment of Agricultural and Environmental Sciences, Milan State University, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Milan State University, 20142 Milan, ItalyLaboratory of Microbiology and Molecular Biology—LMBM, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilLaboratory of Applied Mycology of Cariri, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilInfections by multiresistant microorganisms have led to a continuous investigation of substances acting as modifiers of this resistance. By following this approach, the chemical composition of the essential oil from <i>Piper caldense</i> leaf and its antimicrobial potential were investigated. The antimicrobial activity was determined by broth microdilution method providing values for minimum inhibitory concentration (MIC), IC<sub>50</sub>, and minimum fungicidal concentration (MFC). The essential oil was tested as a modulator for several antibiotics, and its effect on the morphology of <i>Candida</i> <i>albicans</i> (CA) strains was also investigated. The chemical characterization revealed an oil composed mainly of sesquiterpenes. Among them are caryophyllene oxide (13.9%), spathulenol (9.1%), δ-cadinene (7.6%) and bicyclogermacrene (6.7%) with the highest concentrations. The essential oil showed very low activity against the strains of CA with the lowest values for IC<sub>50</sub> and MFC of 1790 μg/mL and 8192 μg/mL, respectively. The essential oil modulated the activity of fluconazole against CA URM 4387 strain, which was demonstrated by the lower IC<sub>50</sub> obtained, 2.7 μg/mL, whereas fluconazole itself presented an IC<sub>50</sub> of 7.76 μg/mL. No modulating effect was observed in the MFC bioassays. The effect on fungal morphology was observed for both CA INCQS 40006 and URM 4387 strains. The hyphae projection was completely inhibited at 4096 μg/mL and 2048 μg/mL, respectively. Thus, the oil has potential as an adjuvant in antimicrobial formulations.https://www.mdpi.com/2076-3417/10/2/631pimenta d’água<i>candida</i>fungistatic effectinhibition of dimorphismgc/ms |
| spellingShingle | José Weverton Almeida Bezerra Felicidade Caroline Rodrigues Rafael Pereira da Cruz Luiz Everson da Silva Wanderlei do Amaral Ricardo Andrade Rebelo Ieda Maria Begnini Camila Fonseca Bezerra Marcello Iriti Elena Maria Varoni Henrique Douglas Melo Coutinho Maria Flaviana Bezerra Morais-Braga Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae) Applied Sciences pimenta d’água <i>candida</i> fungistatic effect inhibition of dimorphism gc/ms |
| title | Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae) |
| title_full | Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae) |
| title_fullStr | Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae) |
| title_full_unstemmed | Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae) |
| title_short | Antibiotic Potential and Chemical Composition of the Essential Oil of <i>Piper caldense</i> C. DC. (Piperaceae) |
| title_sort | antibiotic potential and chemical composition of the essential oil of i piper caldense i c dc piperaceae |
| topic | pimenta d’água <i>candida</i> fungistatic effect inhibition of dimorphism gc/ms |
| url | https://www.mdpi.com/2076-3417/10/2/631 |
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