A preliminary study on the establishment of an animal model of conjunctivochalasis
AIM: To explore a feasible method on the establishment of an animal model of conjunctivochalasis (CCH). METHODS: Twelve clean-grade New Zealand white rabbits were divided into four groups (n=3/group): the control group (one received no interventions, and the others underwent subconjunctival injecti...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-06-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2018/6/20180601.pdf |
| _version_ | 1828421232778805248 |
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| author | Jing-Yun Gan Qing-Song Li Huan-Ming Zhou Wei Zhang Ling-Zhi Lian Zhang Yu Zhen-Yong Zhang |
| author_facet | Jing-Yun Gan Qing-Song Li Huan-Ming Zhou Wei Zhang Ling-Zhi Lian Zhang Yu Zhen-Yong Zhang |
| author_sort | Jing-Yun Gan |
| collection | DOAJ |
| description | AIM: To explore a feasible method on the establishment of an animal model of conjunctivochalasis (CCH).
METHODS: Twelve clean-grade New Zealand white rabbits were divided into four groups (n=3/group): the control group (one received no interventions, and the others underwent subconjunctival injection of sterile water), the matrix metalloproteinases (MMPs) group (administered subconjunctival injection of MMP-3), the aging group (administered subcutaneous injection of D-galactose), the tumor necrosis factor-α (TNF-α) solution group (administered eye drops of TNF-α). Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy (TEM) were performed after 12wk.
RESULTS: Among all groups, the MMPs group had the following changes: the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers (Trichrome staining) and the broken elastic fibers (Aldehyde-fuchsin staining); the focal necrosis of fibroblasts (TEM).
CONCLUSION: Administration of MMPs may be a feasible method for the establishment of an animal model of CCH. |
| first_indexed | 2024-12-10T15:25:56Z |
| format | Article |
| id | doaj.art-5555826214354a1db1c376d8b0b1285a |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-10T15:25:56Z |
| publishDate | 2018-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-5555826214354a1db1c376d8b0b1285a2022-12-22T01:43:33ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-06-0111689990410.18240/ijo.2018.06.01A preliminary study on the establishment of an animal model of conjunctivochalasisJing-Yun Gan0Qing-Song Li1Huan-Ming Zhou2Wei Zhang3Ling-Zhi Lian4Zhang Yu5Zhen-Yong Zhang6Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, ChinaDepartment of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, ChinaDepartment of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, ChinaDepartment of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, ChinaDepartment of Pathology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, ChinaDepartment of Electron Microscopy, Shanghai Medical College, Fudan University, Shanghai 200062, ChinaDepartment of Pathology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, ChinaAIM: To explore a feasible method on the establishment of an animal model of conjunctivochalasis (CCH). METHODS: Twelve clean-grade New Zealand white rabbits were divided into four groups (n=3/group): the control group (one received no interventions, and the others underwent subconjunctival injection of sterile water), the matrix metalloproteinases (MMPs) group (administered subconjunctival injection of MMP-3), the aging group (administered subcutaneous injection of D-galactose), the tumor necrosis factor-α (TNF-α) solution group (administered eye drops of TNF-α). Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy (TEM) were performed after 12wk. RESULTS: Among all groups, the MMPs group had the following changes: the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers (Trichrome staining) and the broken elastic fibers (Aldehyde-fuchsin staining); the focal necrosis of fibroblasts (TEM). CONCLUSION: Administration of MMPs may be a feasible method for the establishment of an animal model of CCH.http://www.ijo.cn/en_publish/2018/6/20180601.pdf904animal modelconjunctivochalasisextracellular matrixinflammatory factorsmatrix metalloproteinase |
| spellingShingle | Jing-Yun Gan Qing-Song Li Huan-Ming Zhou Wei Zhang Ling-Zhi Lian Zhang Yu Zhen-Yong Zhang A preliminary study on the establishment of an animal model of conjunctivochalasis International Journal of Ophthalmology 904 animal model conjunctivochalasis extracellular matrix inflammatory factors matrix metalloproteinase |
| title | A preliminary study on the establishment of an animal model of conjunctivochalasis |
| title_full | A preliminary study on the establishment of an animal model of conjunctivochalasis |
| title_fullStr | A preliminary study on the establishment of an animal model of conjunctivochalasis |
| title_full_unstemmed | A preliminary study on the establishment of an animal model of conjunctivochalasis |
| title_short | A preliminary study on the establishment of an animal model of conjunctivochalasis |
| title_sort | preliminary study on the establishment of an animal model of conjunctivochalasis |
| topic | 904 animal model conjunctivochalasis extracellular matrix inflammatory factors matrix metalloproteinase |
| url | http://www.ijo.cn/en_publish/2018/6/20180601.pdf |
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