Harnessing bioluminescence for drug discovery and epigenetic research
The naturally occurring phenomenon of bioluminescence has intrigued on-lookers for decades and is now being developed as a powerful tool for medical research and preclinical imaging. Luciferase enzymes emit light upon substrate encounter, enabling their activity to be visualised and dynamically trac...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Drug Discovery |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fddsv.2023.1249507/full |
| _version_ | 1827161255596523520 |
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| author | Hannah J. Gleneadie Andrew Dimond Amanda G. Fisher Amanda G. Fisher |
| author_facet | Hannah J. Gleneadie Andrew Dimond Amanda G. Fisher Amanda G. Fisher |
| author_sort | Hannah J. Gleneadie |
| collection | DOAJ |
| description | The naturally occurring phenomenon of bioluminescence has intrigued on-lookers for decades and is now being developed as a powerful tool for medical research and preclinical imaging. Luciferase enzymes emit light upon substrate encounter, enabling their activity to be visualised and dynamically tracked. By inserting luciferase genes into specific sites in the genome, it is possible to engineer reporters to monitor gene expression in its native context, and to detect epigenetic change in vivo. Endogenous bioluminescent reporters provide a highly sensitive, quantitative read-out of gene expression that is both well suited to longitudinal studies and can be adapted for high-throughput drug screens. In this article we outline some of the applications and benefits of bioluminescent reporters for epigenetic research, with a particular focus on revealing new therapeutic options for treating genetic and epigenetic disorders. |
| first_indexed | 2024-03-12T02:28:53Z |
| format | Article |
| id | doaj.art-5558af16256c41448270066153e44e08 |
| institution | Directory Open Access Journal |
| issn | 2674-0338 |
| language | English |
| last_indexed | 2025-03-21T00:26:01Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Drug Discovery |
| spelling | doaj.art-5558af16256c41448270066153e44e082024-08-03T07:13:34ZengFrontiers Media S.A.Frontiers in Drug Discovery2674-03382023-09-01310.3389/fddsv.2023.12495071249507Harnessing bioluminescence for drug discovery and epigenetic researchHannah J. Gleneadie0Andrew Dimond1Amanda G. Fisher2Amanda G. Fisher3Epigenetic Memory Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus, London, United KingdomEpigenetic Memory Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus, London, United KingdomEpigenetic Memory Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus, London, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomThe naturally occurring phenomenon of bioluminescence has intrigued on-lookers for decades and is now being developed as a powerful tool for medical research and preclinical imaging. Luciferase enzymes emit light upon substrate encounter, enabling their activity to be visualised and dynamically tracked. By inserting luciferase genes into specific sites in the genome, it is possible to engineer reporters to monitor gene expression in its native context, and to detect epigenetic change in vivo. Endogenous bioluminescent reporters provide a highly sensitive, quantitative read-out of gene expression that is both well suited to longitudinal studies and can be adapted for high-throughput drug screens. In this article we outline some of the applications and benefits of bioluminescent reporters for epigenetic research, with a particular focus on revealing new therapeutic options for treating genetic and epigenetic disorders.https://www.frontiersin.org/articles/10.3389/fddsv.2023.1249507/fullepigeneticsin vivo imagingbioluminescenceendogenous reporter genesluciferaseepidrugs |
| spellingShingle | Hannah J. Gleneadie Andrew Dimond Amanda G. Fisher Amanda G. Fisher Harnessing bioluminescence for drug discovery and epigenetic research Frontiers in Drug Discovery epigenetics in vivo imaging bioluminescence endogenous reporter genes luciferase epidrugs |
| title | Harnessing bioluminescence for drug discovery and epigenetic research |
| title_full | Harnessing bioluminescence for drug discovery and epigenetic research |
| title_fullStr | Harnessing bioluminescence for drug discovery and epigenetic research |
| title_full_unstemmed | Harnessing bioluminescence for drug discovery and epigenetic research |
| title_short | Harnessing bioluminescence for drug discovery and epigenetic research |
| title_sort | harnessing bioluminescence for drug discovery and epigenetic research |
| topic | epigenetics in vivo imaging bioluminescence endogenous reporter genes luciferase epidrugs |
| url | https://www.frontiersin.org/articles/10.3389/fddsv.2023.1249507/full |
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