| Summary: | <i>Zinc finger BED-type containing 6</i> (<i>ZBED6</i>), a highly conservative transcription factor of placental mammals, has conservative interaction of insulin-like growth factor 2 (<i>IGF2</i>) based on the 16 bp binding sites of <i>ZBED6</i> on the <i>IGF2</i> sequence. IGF2 is related to embryo growth and cell proliferation. At the same time, its functions in muscle and adipose in mammals have been widely mentioned in recent studies. To further investigate the mechanism of <i>ZBED6</i> on <i>IGF2</i>, we detected the expression of <i>IGF2</i> and related genes in <i>ZBED6</i> single allele knockout (<i>ZBED6</i>-SKO) pig tissues and analyzed the transcriptome of <i>ZBED6</i>-SKO pig liver. Through RNA-seq, we captured nine up-regulated genes and eight down-regulated genes which related to lipid metabolism. The results showed that the mRNA of <i>IGF2</i> had an upward trend after the partial knockout of <i>ZBED6</i> in liver and had no significant difference in protein expression of IGF2. In summary, <i>ZBED6</i>-SKO could affect the secretion of IGF2 in pig liver and its own lipid metabolism. Our research has provided basic information for revealing the regulatory mechanism of the interaction between ZBED6 and IGF2 in mammals.
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