In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli
Aim: The aim was to investigate antibacterial activity of Withania somnifera (Ashwagandha), an Indian traditional medicinal plant against Escherichia coli O78, a pathogenic strain. Materials and Methods: Two-fold serial dilutions of 20% aqueous W. somnifera root (WSR) extract were inoculated with E...
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| Format: | Article |
| Language: | English |
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Veterinary World
2015-01-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.8/January-2015/12.pdf |
| _version_ | 1818777574932742144 |
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| author | Mamta Kumari R. P. Gupta |
| author_facet | Mamta Kumari R. P. Gupta |
| author_sort | Mamta Kumari |
| collection | DOAJ |
| description | Aim: The aim was to investigate antibacterial activity of Withania somnifera (Ashwagandha), an Indian traditional medicinal plant against Escherichia coli O78, a pathogenic strain.
Materials and Methods: Two-fold serial dilutions of 20% aqueous W. somnifera root (WSR) extract were inoculated with E. coli O78 @ 1x107 colony forming units grown in nutrient broth. Following inoculation, turbidity optical density was measured by spectrophotometer at 600 nm in all the tubes at 0, 2, 4, 6 and 8 h of incubation at 37°C.
Result: The results revealed that the maximum inhibition of bacterial growth was observed at 1:8 dilution of WSR extract. The highest dilution of the extract that showed inhibited growth of the test organism when compared with control was 1:16. Therefore, the minimum inhibitory concentration of aqueous extract of WSR is 1:16.
Conclusion: It is concluded that WSR possessed good antibacterial activity, confirming the great potential of bioactive compounds and its rationalizing use in health care. |
| first_indexed | 2024-12-18T11:31:00Z |
| format | Article |
| id | doaj.art-555ca38824e9470f949d444bba8cfe03 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 0972-8988 |
| language | English |
| last_indexed | 2024-12-18T11:31:00Z |
| publishDate | 2015-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-555ca38824e9470f949d444bba8cfe032022-12-21T21:09:36ZengVeterinary WorldVeterinary World0972-89880972-89882015-01-0181576010.14202/vetworld.2015.57-60In vitro antibacterial effect of Withania somnifera root extract on Escherichia coliMamta Kumari 0R. P. Gupta1Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India; mamtabijarnia@gmail.comDepartment of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India; rpgupta2007@yahoo.co.inAim: The aim was to investigate antibacterial activity of Withania somnifera (Ashwagandha), an Indian traditional medicinal plant against Escherichia coli O78, a pathogenic strain. Materials and Methods: Two-fold serial dilutions of 20% aqueous W. somnifera root (WSR) extract were inoculated with E. coli O78 @ 1x107 colony forming units grown in nutrient broth. Following inoculation, turbidity optical density was measured by spectrophotometer at 600 nm in all the tubes at 0, 2, 4, 6 and 8 h of incubation at 37°C. Result: The results revealed that the maximum inhibition of bacterial growth was observed at 1:8 dilution of WSR extract. The highest dilution of the extract that showed inhibited growth of the test organism when compared with control was 1:16. Therefore, the minimum inhibitory concentration of aqueous extract of WSR is 1:16. Conclusion: It is concluded that WSR possessed good antibacterial activity, confirming the great potential of bioactive compounds and its rationalizing use in health care.http://www.veterinaryworld.org/Vol.8/January-2015/12.pdfantibacterialAshwagandhaWithania somniferaroot extract |
| spellingShingle | Mamta Kumari R. P. Gupta In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli Veterinary World antibacterial Ashwagandha Withania somnifera root extract |
| title | In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli |
| title_full | In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli |
| title_fullStr | In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli |
| title_full_unstemmed | In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli |
| title_short | In vitro antibacterial effect of Withania somnifera root extract on Escherichia coli |
| title_sort | in vitro antibacterial effect of withania somnifera root extract on escherichia coli |
| topic | antibacterial Ashwagandha Withania somnifera root extract |
| url | http://www.veterinaryworld.org/Vol.8/January-2015/12.pdf |
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