Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus
This study was carried out to analyze the effects of biogenic silver nanoparticles (AgNPs) on physiological, biochemical, and enzymatic attributes of rice plants against Aspergillus flavus. The plant-based AgNPs were synthesized by using the aqueous extract of Moringa oleifera leaves. The characteri...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-05-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2021-0034 |
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| author | Sultana Tahira Javed Bilal Raja Naveed Iqbal Mashwani Zia-ur-Rehman |
| author_facet | Sultana Tahira Javed Bilal Raja Naveed Iqbal Mashwani Zia-ur-Rehman |
| author_sort | Sultana Tahira |
| collection | DOAJ |
| description | This study was carried out to analyze the effects of biogenic silver nanoparticles (AgNPs) on physiological, biochemical, and enzymatic attributes of rice plants against Aspergillus flavus. The plant-based AgNPs were synthesized by using the aqueous extract of Moringa oleifera leaves. The characterization of AgNPs was accomplished through UV-visible spectrophotometry, SEM, and energy-dispersive X-ray (EDX) analysis, which confirmed that the nanoparticles are crystalline and are less than 100 nm in size. The exogenous applications of different concentrations of AgNPs (25, 50, 75, and 100 mg/L) on rice plants in field experiments were used to control the proliferation of A. flavus. The effects of biosynthesized AgNPs were evaluated for physiological (relative water content, membrane stability index, and chlorophyll content), nonenzymatic metabolites (total phenolic, total flavonoid, proline, soluble sugar, and protein contents), and enzymatic metabolites (superoxide dismutase, peroxidase, and catalase) in rice plants under biotic stress, and 50 mg/L concentration of AgNPs was found to be effective to elicit biochemical modifications to reduce biotic stress. The 50 mg/L concentration of AgNPs was also effective in controlling the proliferation of fungal pathogen. The applications of AgNPs reduced the biotic stress by decreasing the production level of osmolytes, enzymatic, and nonenzymatic compounds but significantly increased the protein content. |
| first_indexed | 2024-04-11T19:37:14Z |
| format | Article |
| id | doaj.art-555e8fe931e741a09f3b803b5f802698 |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-11T19:37:14Z |
| publishDate | 2021-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-555e8fe931e741a09f3b803b5f8026982022-12-22T04:06:49ZengDe GruyterGreen Processing and Synthesis2191-95502021-05-0110131432410.1515/gps-2021-0034Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavusSultana Tahira0Javed Bilal1Raja Naveed Iqbal2Mashwani Zia-ur-Rehman3Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanThis study was carried out to analyze the effects of biogenic silver nanoparticles (AgNPs) on physiological, biochemical, and enzymatic attributes of rice plants against Aspergillus flavus. The plant-based AgNPs were synthesized by using the aqueous extract of Moringa oleifera leaves. The characterization of AgNPs was accomplished through UV-visible spectrophotometry, SEM, and energy-dispersive X-ray (EDX) analysis, which confirmed that the nanoparticles are crystalline and are less than 100 nm in size. The exogenous applications of different concentrations of AgNPs (25, 50, 75, and 100 mg/L) on rice plants in field experiments were used to control the proliferation of A. flavus. The effects of biosynthesized AgNPs were evaluated for physiological (relative water content, membrane stability index, and chlorophyll content), nonenzymatic metabolites (total phenolic, total flavonoid, proline, soluble sugar, and protein contents), and enzymatic metabolites (superoxide dismutase, peroxidase, and catalase) in rice plants under biotic stress, and 50 mg/L concentration of AgNPs was found to be effective to elicit biochemical modifications to reduce biotic stress. The 50 mg/L concentration of AgNPs was also effective in controlling the proliferation of fungal pathogen. The applications of AgNPs reduced the biotic stress by decreasing the production level of osmolytes, enzymatic, and nonenzymatic compounds but significantly increased the protein content.https://doi.org/10.1515/gps-2021-0034agnpsricegreen synthesisbiochemical profilingenzymatic assays |
| spellingShingle | Sultana Tahira Javed Bilal Raja Naveed Iqbal Mashwani Zia-ur-Rehman Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus Green Processing and Synthesis agnps rice green synthesis biochemical profiling enzymatic assays |
| title | Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus |
| title_full | Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus |
| title_fullStr | Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus |
| title_full_unstemmed | Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus |
| title_short | Silver nanoparticles elicited physiological, biochemical, and antioxidant modifications in rice plants to control Aspergillus flavus |
| title_sort | silver nanoparticles elicited physiological biochemical and antioxidant modifications in rice plants to control aspergillus flavus |
| topic | agnps rice green synthesis biochemical profiling enzymatic assays |
| url | https://doi.org/10.1515/gps-2021-0034 |
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