Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae
Many gram-negative bacteria secrete so-called effector proteins via a type III secretion (T3S) system. Through genome screening for genes encoding potential T3S effectors, 60 candidates were selected from rice pathogen Xanthomonas oryzae pv. oryzae MAFF311018 using these criteria: i) homologs of kno...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-01-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-1-0096 |
| _version_ | 1818915226572029952 |
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| author | Ayako Furutani Minako Takaoka Harumi Sanada Yukari Noguchi Takashi Oku Kazunori Tsuno Hirokazu Ochiai Seiji Tsuge |
| author_facet | Ayako Furutani Minako Takaoka Harumi Sanada Yukari Noguchi Takashi Oku Kazunori Tsuno Hirokazu Ochiai Seiji Tsuge |
| author_sort | Ayako Furutani |
| collection | DOAJ |
| description | Many gram-negative bacteria secrete so-called effector proteins via a type III secretion (T3S) system. Through genome screening for genes encoding potential T3S effectors, 60 candidates were selected from rice pathogen Xanthomonas oryzae pv. oryzae MAFF311018 using these criteria: i) homologs of known T3S effectors in plant-pathogenic bacteria, ii) genes with expression regulated by hrp regulatory protein HrpX, or iii) proteins with N-terminal amino acid patterns associated with T3S substrates of Pseudomonas syringae. Of effector candidates tested with the Bordetella pertussis calmodulin-dependent adenylate cyclase reporter for translocation into plant cells, 16 proteins were translocated in a T3S system-dependent manner. Of these 16 proteins, nine were homologs of known effectors in other plant-pathogenic bacteria and seven were not. Most of the effectors were widely conserved in Xanthomonas spp.; however, some were specific to X. oryzae. Interestingly, all these effectors were expressed in an HrpX-dependent manner, suggesting coregulation of effectors and the T3S system. In X. campestris pv. vesicatoria, HpaB and HpaC (HpaP in X. oryzae pv. oryzae) have a central role in recruiting T3S substrates to the secretion apparatus. Secretion of all but one effector was reduced in both HpaB– and HpaP– mutant strains, indicating that HpaB and HpaP are widely involved in efficient secretion of the effectors. |
| first_indexed | 2024-12-19T23:58:55Z |
| format | Article |
| id | doaj.art-5560911cc6894d6f815dfcffbe714f1d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:58:55Z |
| publishDate | 2009-01-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-5560911cc6894d6f815dfcffbe714f1d2022-12-21T20:00:55ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-01-012219610610.1094/MPMI-22-1-0096Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzaeAyako FurutaniMinako TakaokaHarumi SanadaYukari NoguchiTakashi OkuKazunori TsunoHirokazu OchiaiSeiji TsugeMany gram-negative bacteria secrete so-called effector proteins via a type III secretion (T3S) system. Through genome screening for genes encoding potential T3S effectors, 60 candidates were selected from rice pathogen Xanthomonas oryzae pv. oryzae MAFF311018 using these criteria: i) homologs of known T3S effectors in plant-pathogenic bacteria, ii) genes with expression regulated by hrp regulatory protein HrpX, or iii) proteins with N-terminal amino acid patterns associated with T3S substrates of Pseudomonas syringae. Of effector candidates tested with the Bordetella pertussis calmodulin-dependent adenylate cyclase reporter for translocation into plant cells, 16 proteins were translocated in a T3S system-dependent manner. Of these 16 proteins, nine were homologs of known effectors in other plant-pathogenic bacteria and seven were not. Most of the effectors were widely conserved in Xanthomonas spp.; however, some were specific to X. oryzae. Interestingly, all these effectors were expressed in an HrpX-dependent manner, suggesting coregulation of effectors and the T3S system. In X. campestris pv. vesicatoria, HpaB and HpaC (HpaP in X. oryzae pv. oryzae) have a central role in recruiting T3S substrates to the secretion apparatus. Secretion of all but one effector was reduced in both HpaB– and HpaP– mutant strains, indicating that HpaB and HpaP are widely involved in efficient secretion of the effectors.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-1-0096 |
| spellingShingle | Ayako Furutani Minako Takaoka Harumi Sanada Yukari Noguchi Takashi Oku Kazunori Tsuno Hirokazu Ochiai Seiji Tsuge Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae Molecular Plant-Microbe Interactions |
| title | Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae |
| title_full | Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae |
| title_fullStr | Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae |
| title_full_unstemmed | Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae |
| title_short | Identification of Novel Type III Secretion Effectors in Xanthomonas oryzae pv. oryzae |
| title_sort | identification of novel type iii secretion effectors in xanthomonas oryzae pv oryzae |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-1-0096 |
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