Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins
Advances in the life sciences are now closely linked to the availability of new experimental tools that allow for the manipulation of biomolecules and the study of biological processes at the molecular level. In this context, we have optimized a synthesis process to obtain glutathione‐capped fluores...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi - SAGE Publishing
2012-10-01
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| Series: | Nanomaterials and Nanotechnology |
| Subjects: | |
| Online Access: | http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/preparation-and-characterization-of-fluorescent-cds-quantum-dots-used-for-the-direct-detection-of-gs |
| _version_ | 1797641609609740288 |
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| author | J.J. Beato-López C. Fernández-Ponce E. Blanco C. Barrera-Solano M. Ramírez-del-Solar M. Domínguez F. García-Cozar R. Litrán |
| author_facet | J.J. Beato-López C. Fernández-Ponce E. Blanco C. Barrera-Solano M. Ramírez-del-Solar M. Domínguez F. García-Cozar R. Litrán |
| author_sort | J.J. Beato-López |
| collection | DOAJ |
| description | Advances in the life sciences are now closely linked
to the availability of new experimental tools that allow for
the manipulation of biomolecules and the study of biological
processes at the molecular level. In this context, we have
optimized a synthesis process to obtain glutathione‐capped
fluorescent CdS nanoparticles to specifically detect
Glutathione S‐Transferase (GST) ‐tagged proteins. Using our
method, based on five different heating steps, brightly
fluorescent and biocompatible CdS quantum dots of
different sizes can be obtained. QD optical behaviour has
been evaluated studying both absorbance and fluorescence.
For all the samples, the excitonic absorption onset clearly
shows a blue shift at 512nm in comparison with that of bulk
CdS, due to the quantum confinement effect. At increased
average sizes of the nanocrystal, the emission fluorescent
band shows a red shift, from 440nm to 540nm. Among
different QD solutions, we demonstrate an expansion of the
emission range up to ~100 nm, thus improving their features
as biomarkers. Moreover we show that optimized glutathionecapped
quantum dots can directly bind GST blotted onto
polyvinylidene difluoride (PVDF) membranes, and thus are
suitable for the direct detection of GST fusion proteins. |
| first_indexed | 2024-03-11T13:48:08Z |
| format | Article |
| id | doaj.art-556294cb569c4259809c3004b2f348bb |
| institution | Directory Open Access Journal |
| issn | 1847-9804 |
| language | English |
| last_indexed | 2024-03-11T13:48:08Z |
| publishDate | 2012-10-01 |
| publisher | Hindawi - SAGE Publishing |
| record_format | Article |
| series | Nanomaterials and Nanotechnology |
| spelling | doaj.art-556294cb569c4259809c3004b2f348bb2023-11-02T09:36:27ZengHindawi - SAGE PublishingNanomaterials and Nanotechnology1847-98042012-10-01210http://dx.doi.org/10.5772/5392639577Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion ProteinsJ.J. Beato-LópezC. Fernández-PonceE. BlancoC. Barrera-SolanoM. Ramírez-del-SolarM. DomínguezF. García-CozarR. LitránAdvances in the life sciences are now closely linked to the availability of new experimental tools that allow for the manipulation of biomolecules and the study of biological processes at the molecular level. In this context, we have optimized a synthesis process to obtain glutathione‐capped fluorescent CdS nanoparticles to specifically detect Glutathione S‐Transferase (GST) ‐tagged proteins. Using our method, based on five different heating steps, brightly fluorescent and biocompatible CdS quantum dots of different sizes can be obtained. QD optical behaviour has been evaluated studying both absorbance and fluorescence. For all the samples, the excitonic absorption onset clearly shows a blue shift at 512nm in comparison with that of bulk CdS, due to the quantum confinement effect. At increased average sizes of the nanocrystal, the emission fluorescent band shows a red shift, from 440nm to 540nm. Among different QD solutions, we demonstrate an expansion of the emission range up to ~100 nm, thus improving their features as biomarkers. Moreover we show that optimized glutathionecapped quantum dots can directly bind GST blotted onto polyvinylidene difluoride (PVDF) membranes, and thus are suitable for the direct detection of GST fusion proteins.http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/preparation-and-characterization-of-fluorescent-cds-quantum-dots-used-for-the-direct-detection-of-gsquantum dotsfluorescenceglutathioneglutathione S‐transferase fusion protein |
| spellingShingle | J.J. Beato-López C. Fernández-Ponce E. Blanco C. Barrera-Solano M. Ramírez-del-Solar M. Domínguez F. García-Cozar R. Litrán Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins Nanomaterials and Nanotechnology quantum dots fluorescence glutathione glutathione S‐transferase fusion protein |
| title | Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins |
| title_full | Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins |
| title_fullStr | Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins |
| title_full_unstemmed | Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins |
| title_short | Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins |
| title_sort | preparation and characterization of fluorescent cds quantum dots used for the direct detection of gst fusion proteins |
| topic | quantum dots fluorescence glutathione glutathione S‐transferase fusion protein |
| url | http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/preparation-and-characterization-of-fluorescent-cds-quantum-dots-used-for-the-direct-detection-of-gs |
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