Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer
Background/Aims: MicroRNAs (miRNAs) are reported to regulate cell invasion and functions by interfering with the translation of target mRNAs, but the role of miRNAs in esophageal cancer (EC) remains unclear. Methods: RT-PCR and Western blot were used to detect the expression of miRNAs and candidate...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-10-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/366351 |
| _version_ | 1819101672621735936 |
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| author | Weiling Li Guojun Jiang Jianping Zhou Hongmin Wang Zegang Gong Zheng Zhang Ke Min Hongdi Zhu Yongfei Tan |
| author_facet | Weiling Li Guojun Jiang Jianping Zhou Hongmin Wang Zegang Gong Zheng Zhang Ke Min Hongdi Zhu Yongfei Tan |
| author_sort | Weiling Li |
| collection | DOAJ |
| description | Background/Aims: MicroRNAs (miRNAs) are reported to regulate cell invasion and functions by interfering with the translation of target mRNAs, but the role of miRNAs in esophageal cancer (EC) remains unclear. Methods: RT-PCR and Western blot were used to detect the expression of miRNAs and candidate genes in EC samples (n=89). miR-140 mimics and inhibitor were tansfected in human TE-1 and Eca-109 cells. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter assay. The markers of EMT were detected by using Western blot. Results: miR-140 expression was decreased in the EC tissues compared with the corresponding adjacent tumor tissues. Low expression of miR-140 promotes cell invasion by using transwell assay, while the effect of miR-140 high expression is reverse. Slug, a target gene of miR-140, was examined by luciferase assay and Western blot. Conclusions: miR-140 may regulate the cell invasion of EC via controlling Slug expression. |
| first_indexed | 2024-12-22T01:22:24Z |
| format | Article |
| id | doaj.art-55631b2c56074d25b44ccb1b9b5d8729 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T01:22:24Z |
| publishDate | 2014-10-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-55631b2c56074d25b44ccb1b9b5d87292022-12-21T18:43:42ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-10-013451466147610.1159/000366351366351Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal CancerWeiling LiGuojun JiangJianping ZhouHongmin WangZegang GongZheng ZhangKe MinHongdi ZhuYongfei TanBackground/Aims: MicroRNAs (miRNAs) are reported to regulate cell invasion and functions by interfering with the translation of target mRNAs, but the role of miRNAs in esophageal cancer (EC) remains unclear. Methods: RT-PCR and Western blot were used to detect the expression of miRNAs and candidate genes in EC samples (n=89). miR-140 mimics and inhibitor were tansfected in human TE-1 and Eca-109 cells. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter assay. The markers of EMT were detected by using Western blot. Results: miR-140 expression was decreased in the EC tissues compared with the corresponding adjacent tumor tissues. Low expression of miR-140 promotes cell invasion by using transwell assay, while the effect of miR-140 high expression is reverse. Slug, a target gene of miR-140, was examined by luciferase assay and Western blot. Conclusions: miR-140 may regulate the cell invasion of EC via controlling Slug expression.http://www.karger.com/Article/FullText/366351miR-140SlugInvasionEsophageal cancerEMT |
| spellingShingle | Weiling Li Guojun Jiang Jianping Zhou Hongmin Wang Zegang Gong Zheng Zhang Ke Min Hongdi Zhu Yongfei Tan Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer Cellular Physiology and Biochemistry miR-140 Slug Invasion Esophageal cancer EMT |
| title | Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer |
| title_full | Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer |
| title_fullStr | Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer |
| title_full_unstemmed | Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer |
| title_short | Down-Regulation of miR-140 Induces EMT and Promotes Invasion by Targeting Slug in Esophageal Cancer |
| title_sort | down regulation of mir 140 induces emt and promotes invasion by targeting slug in esophageal cancer |
| topic | miR-140 Slug Invasion Esophageal cancer EMT |
| url | http://www.karger.com/Article/FullText/366351 |
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