Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer
In the quest for the development of accurate, reliable, and cost-effective biosensing technology for early diagnostics of prostate cancer, we describe here an electrochemical biosensor combining a simple transducing method of differential pulse voltammetry (DPV) with an RNA-based aptamer labelled wi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Eng |
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| Online Access: | https://www.mdpi.com/2673-4117/4/1/22 |
| _version_ | 1797612022395830272 |
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| author | Sarra Takita Alexei Nabok Anna Lishchuk Magdi H. Mussa David Smith |
| author_facet | Sarra Takita Alexei Nabok Anna Lishchuk Magdi H. Mussa David Smith |
| author_sort | Sarra Takita |
| collection | DOAJ |
| description | In the quest for the development of accurate, reliable, and cost-effective biosensing technology for early diagnostics of prostate cancer, we describe here an electrochemical biosensor combining a simple transducing method of differential pulse voltammetry (DPV) with an RNA-based aptamer labelled with a methylene blue redox group acting as a highly specific bioreceptor to the prostate cancer biomarker PCA3. A series of DPV measurements on screen-printed gold electrodes is functionalised with a redox-labelled aptamer in solutions (either buffer or synthetic urine) containing PCA3 in a wide range of concentrations from 0.1 picomolar (pM) to 10 nanomolar (nM). In these measurements, the current peak values correlate with the concentration of PCA3 and yield a low detection limit (LDL) of 0.1 pM. Furthermore, the binding kinetics study revealed the high affinity of the aptamer to the target PCA3 with the affinity constants K<sub>D</sub> of about 3.0 × 10<sup>−8</sup> molar. In addition, the AFM study showed the increase in the molecular layer roughness caused by the binding of PCA3, which is a large RNA molecular fragment. |
| first_indexed | 2024-03-11T06:35:47Z |
| format | Article |
| id | doaj.art-55659d9332874d5db30ec30e1b04a0e9 |
| institution | Directory Open Access Journal |
| issn | 2673-4117 |
| language | English |
| last_indexed | 2024-03-11T06:35:47Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Eng |
| spelling | doaj.art-55659d9332874d5db30ec30e1b04a0e92023-11-17T10:53:17ZengMDPI AGEng2673-41172023-01-014136737910.3390/eng4010022Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific AptamerSarra Takita0Alexei Nabok1Anna Lishchuk2Magdi H. Mussa3David Smith4Material and Engineering Research Institute (MERI), Sheffield Hallam University, Sheffield S1 1WB, UKMaterial and Engineering Research Institute (MERI), Sheffield Hallam University, Sheffield S1 1WB, UKDepartment of Chemistry, University of Sheffield, Brook Hill, Sheffield S3 7HF, UKMaterial and Engineering Research Institute (MERI), Sheffield Hallam University, Sheffield S1 1WB, UKBiomolecular Sciences Research Centre (BMRC), Sheffield Hallam University, Sheffield S1 1WB, UKIn the quest for the development of accurate, reliable, and cost-effective biosensing technology for early diagnostics of prostate cancer, we describe here an electrochemical biosensor combining a simple transducing method of differential pulse voltammetry (DPV) with an RNA-based aptamer labelled with a methylene blue redox group acting as a highly specific bioreceptor to the prostate cancer biomarker PCA3. A series of DPV measurements on screen-printed gold electrodes is functionalised with a redox-labelled aptamer in solutions (either buffer or synthetic urine) containing PCA3 in a wide range of concentrations from 0.1 picomolar (pM) to 10 nanomolar (nM). In these measurements, the current peak values correlate with the concentration of PCA3 and yield a low detection limit (LDL) of 0.1 pM. Furthermore, the binding kinetics study revealed the high affinity of the aptamer to the target PCA3 with the affinity constants K<sub>D</sub> of about 3.0 × 10<sup>−8</sup> molar. In addition, the AFM study showed the increase in the molecular layer roughness caused by the binding of PCA3, which is a large RNA molecular fragment.https://www.mdpi.com/2673-4117/4/1/22prostate cancerPCA3 biomarkeraptamerelectrochemical sensorsdigital pulse voltammetrybinding kinetics |
| spellingShingle | Sarra Takita Alexei Nabok Anna Lishchuk Magdi H. Mussa David Smith Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer Eng prostate cancer PCA3 biomarker aptamer electrochemical sensors digital pulse voltammetry binding kinetics |
| title | Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer |
| title_full | Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer |
| title_fullStr | Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer |
| title_full_unstemmed | Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer |
| title_short | Enhanced Performance Electrochemical Biosensor for Detection of Prostate Cancer Biomarker PCA3 Using Specific Aptamer |
| title_sort | enhanced performance electrochemical biosensor for detection of prostate cancer biomarker pca3 using specific aptamer |
| topic | prostate cancer PCA3 biomarker aptamer electrochemical sensors digital pulse voltammetry binding kinetics |
| url | https://www.mdpi.com/2673-4117/4/1/22 |
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