Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR
Cytochrome c (cyt c) is an important multifunctional protein. In mitochondria, it acts as a carrier for electron transporting. In cytoplasm, it may act as an apoptotic initiator to initiate the apoptotic process. Whether and how a complex cytoplasmic environment affects its conformation has not been...
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Science Press
2023-03-01
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| Series: | Chinese Journal of Magnetic Resonance |
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| Online Access: | http://121.43.60.238/bpxzz/article/2023/1000-4556/1000-4556-40-1-22.shtml |
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| author | ZHAN Jianhua 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China ZHU Qinjun JIANG Bin 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, China LIU Maili |
| author_facet | ZHAN Jianhua 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China ZHU Qinjun JIANG Bin 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, China LIU Maili |
| author_sort | ZHAN Jianhua |
| collection | DOAJ |
| description | Cytochrome c (cyt c) is an important multifunctional protein. In mitochondria, it acts as a carrier for electron transporting. In cytoplasm, it may act as an apoptotic initiator to initiate the apoptotic process. Whether and how a complex cytoplasmic environment affects its conformation has not been confirmed yet. In this study, the conformational changes of wild type saccharomyces cerevisiae iso-1 cyt c in yeast cell homogenate were tracked by methyl-based nuclear magnetic resonance (NMR) technique. At least four different oxidative conformations and one reduced conformation of cyt c were identified in cell homogenate. And over time, the transitions among different conformations of cyt c were observed. The results indicate that the conformation of cyt c changes with environment, which may be closely related to resistance to oxidative stress. |
| first_indexed | 2024-04-09T19:10:10Z |
| format | Article |
| id | doaj.art-5567bfd7087645148bbd1102da86fca9 |
| institution | Directory Open Access Journal |
| issn | 1000-4556 |
| language | zho |
| last_indexed | 2024-04-09T19:10:10Z |
| publishDate | 2023-03-01 |
| publisher | Science Press |
| record_format | Article |
| series | Chinese Journal of Magnetic Resonance |
| spelling | doaj.art-5567bfd7087645148bbd1102da86fca92023-04-07T01:41:42ZzhoScience PressChinese Journal of Magnetic Resonance1000-45562023-03-01401222910.11938/cjmr20222985Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMRZHAN Jianhua01. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China1 ZHU Qinjun2JIANG Bin31. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, China4LIU Maili51. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, China1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, China1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, ChinaCytochrome c (cyt c) is an important multifunctional protein. In mitochondria, it acts as a carrier for electron transporting. In cytoplasm, it may act as an apoptotic initiator to initiate the apoptotic process. Whether and how a complex cytoplasmic environment affects its conformation has not been confirmed yet. In this study, the conformational changes of wild type saccharomyces cerevisiae iso-1 cyt c in yeast cell homogenate were tracked by methyl-based nuclear magnetic resonance (NMR) technique. At least four different oxidative conformations and one reduced conformation of cyt c were identified in cell homogenate. And over time, the transitions among different conformations of cyt c were observed. The results indicate that the conformation of cyt c changes with environment, which may be closely related to resistance to oxidative stress.http://121.43.60.238/bpxzz/article/2023/1000-4556/1000-4556-40-1-22.shtmlnuclear magnetic resonance (nmr)conformational changemethylcytochrome c |
| spellingShingle | ZHAN Jianhua 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China ZHU Qinjun JIANG Bin 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China 4. Optics Valley Laboratory, Wuhan 430074, China LIU Maili Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR Chinese Journal of Magnetic Resonance nuclear magnetic resonance (nmr) conformational change methyl cytochrome c |
| title | Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR |
| title_full | Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR |
| title_fullStr | Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR |
| title_full_unstemmed | Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR |
| title_short | Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR |
| title_sort | track the conformational change of unlabeled yeast cytochrome c in cell homogenate using nmr |
| topic | nuclear magnetic resonance (nmr) conformational change methyl cytochrome c |
| url | http://121.43.60.238/bpxzz/article/2023/1000-4556/1000-4556-40-1-22.shtml |
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