Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt
Hydrocotyle umbellata L. is a creeping aquatic herb belonging to family Araliaceae. The plant has been used in folk medicine as an anti-inflammatory, anxiolytic and memory stimulant herb. This study targets the botanical features of the leaf, rhizome and roots, in addition to DNA fingerprinting usin...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2018-06-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093118300073 |
| _version_ | 1797967231980666880 |
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| author | Sherif A. Hamdy Hala M. El Hefnawy Shadia M. Azzam Elsayed A. Aboutabl |
| author_facet | Sherif A. Hamdy Hala M. El Hefnawy Shadia M. Azzam Elsayed A. Aboutabl |
| author_sort | Sherif A. Hamdy |
| collection | DOAJ |
| description | Hydrocotyle umbellata L. is a creeping aquatic herb belonging to family Araliaceae. The plant has been used in folk medicine as an anti-inflammatory, anxiolytic and memory stimulant herb. This study targets the botanical features of the leaf, rhizome and roots, in addition to DNA fingerprinting using rapid amplified (RAPD) and inter simple sequence repeat (ISSR) based polymerase chain reaction (PCR) techniques. Microscopically, leaves and rhizomes are characterized by numerous brown schizogenous ducts containing volatile oils. On the other hand, a total of 34 random amplified polymorphic DNA (RAPD) markers were identified using seven random decamer primers and a total of 15 inter simple sequence repeat (ISSR) markers were identified using five decamer random primers. Both the botanical study and DNA fingerprinting could be useful in identification and authentication of the plant. |
| first_indexed | 2024-04-11T02:26:53Z |
| format | Article |
| id | doaj.art-556917ba53414761bcb849942436af62 |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T02:26:53Z |
| publishDate | 2018-06-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-556917ba53414761bcb849942436af622023-01-02T22:23:45ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312018-06-01561465310.1016/j.bfopcu.2018.03.006Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in EgyptSherif A. HamdyHala M. El HefnawyShadia M. AzzamElsayed A. AboutablHydrocotyle umbellata L. is a creeping aquatic herb belonging to family Araliaceae. The plant has been used in folk medicine as an anti-inflammatory, anxiolytic and memory stimulant herb. This study targets the botanical features of the leaf, rhizome and roots, in addition to DNA fingerprinting using rapid amplified (RAPD) and inter simple sequence repeat (ISSR) based polymerase chain reaction (PCR) techniques. Microscopically, leaves and rhizomes are characterized by numerous brown schizogenous ducts containing volatile oils. On the other hand, a total of 34 random amplified polymorphic DNA (RAPD) markers were identified using seven random decamer primers and a total of 15 inter simple sequence repeat (ISSR) markers were identified using five decamer random primers. Both the botanical study and DNA fingerprinting could be useful in identification and authentication of the plant.http://www.sciencedirect.com/science/article/pii/S1110093118300073Hydrocotyle umbellata L.Schizogenous ductsRAPDISSRDNA fingerprinting |
| spellingShingle | Sherif A. Hamdy Hala M. El Hefnawy Shadia M. Azzam Elsayed A. Aboutabl Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt Bulletin of Faculty of Pharmacy Cairo University Hydrocotyle umbellata L. Schizogenous ducts RAPD ISSR DNA fingerprinting |
| title | Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt |
| title_full | Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt |
| title_fullStr | Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt |
| title_full_unstemmed | Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt |
| title_short | Botanical and genetic characterization of Hydrocotyle umbellata L. cultivated in Egypt |
| title_sort | botanical and genetic characterization of hydrocotyle umbellata l cultivated in egypt |
| topic | Hydrocotyle umbellata L. Schizogenous ducts RAPD ISSR DNA fingerprinting |
| url | http://www.sciencedirect.com/science/article/pii/S1110093118300073 |
| work_keys_str_mv | AT sherifahamdy botanicalandgeneticcharacterizationofhydrocotyleumbellatalcultivatedinegypt AT halamelhefnawy botanicalandgeneticcharacterizationofhydrocotyleumbellatalcultivatedinegypt AT shadiamazzam botanicalandgeneticcharacterizationofhydrocotyleumbellatalcultivatedinegypt AT elsayedaaboutabl botanicalandgeneticcharacterizationofhydrocotyleumbellatalcultivatedinegypt |