Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain
Wastewater treatment plants (WWTPs) are recognized as important niches of antibiotic-resistant bacteria that can be easily spread to the environment. In this study, we collected wastewater samples from the WWTP of A Coruña (NW Spain) from April 2020 to February 2022 to evaluate the presence of Gram-...
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MDPI AG
2024-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/2/194 |
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| author | Mohammed Nasser-Ali Pablo Aja-Macaya Kelly Conde-Pérez Noelia Trigo-Tasende Soraya Rumbo-Feal Ana Fernández-González Germán Bou Margarita Poza Juan A. Vallejo |
| author_facet | Mohammed Nasser-Ali Pablo Aja-Macaya Kelly Conde-Pérez Noelia Trigo-Tasende Soraya Rumbo-Feal Ana Fernández-González Germán Bou Margarita Poza Juan A. Vallejo |
| author_sort | Mohammed Nasser-Ali |
| collection | DOAJ |
| description | Wastewater treatment plants (WWTPs) are recognized as important niches of antibiotic-resistant bacteria that can be easily spread to the environment. In this study, we collected wastewater samples from the WWTP of A Coruña (NW Spain) from April 2020 to February 2022 to evaluate the presence of Gram-negative bacteria harboring carbapenemase genes. Bacteria isolated from wastewater were classified and their antimicrobial profiles were determined. In total, 252 Gram-negative bacteria carrying various carbapenemase genes were described. Whole-genome sequencing was conducted on 55 selected carbapenemase producing isolates using Oxford Nanopore technology. This study revealed the presence of a significant population of bacteria carrying carbapenemase genes in WWTP, which constitutes a public health problem due to their risk of dissemination to the environment. This emphasizes the usefulness of WWTP monitoring for combating antibiotic resistance. Data revealed the presence of different types of sequences harboring carbapenemase genes, such as <i>bla</i><sub>KPC-2</sub>, <i>bla</i><sub>GES-5</sub>, <i>bla</i><sub>GES-6</sub>, <i>bla</i><sub>IMP-11</sub>, <i>bla</i><sub>IMP-28</sub>, <i>bla</i><sub>OXA-24</sub>, <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>OXA-58</sub>, <i>bla</i><sub>OXA-217</sub>, and <i>bla</i><sub>VIM-2</sub>. Importantly, the presence of the <i>bla</i><sub>KPC-2</sub> gene in wastewater, several months before any clinical case was detected in University Hospital of A Coruña, suggests that wastewater-based epidemiology can be used as an early warning system for the surveillance of antibiotic-resistant bacteria. |
| first_indexed | 2024-03-07T22:44:29Z |
| format | Article |
| id | doaj.art-5569518adfd4493b8938372e6f1e3894 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-07T22:44:29Z |
| publishDate | 2024-02-01 |
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| series | Antibiotics |
| spelling | doaj.art-5569518adfd4493b8938372e6f1e38942024-02-23T15:05:14ZengMDPI AGAntibiotics2079-63822024-02-0113219410.3390/antibiotics13020194Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, SpainMohammed Nasser-Ali0Pablo Aja-Macaya1Kelly Conde-Pérez2Noelia Trigo-Tasende3Soraya Rumbo-Feal4Ana Fernández-González5Germán Bou6Margarita Poza7Juan A. Vallejo8Microbiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainMicrobiology Research Group, Institute of Biomedical Research (INIBIC)-University Hospital of A Coruña (CHUAC)-Interdisciplinary Center for Chemistry and Biology (CICA)-University of A Coruña (UDC)-CIBER de Enfermedades Infecciosas (CIBERINFEC, ISCIII). Servicio de Microbiología, 3° planta, Edificio Sur, Hospital Universitario, As Xubias, 15006 A Coruna, SpainWastewater treatment plants (WWTPs) are recognized as important niches of antibiotic-resistant bacteria that can be easily spread to the environment. In this study, we collected wastewater samples from the WWTP of A Coruña (NW Spain) from April 2020 to February 2022 to evaluate the presence of Gram-negative bacteria harboring carbapenemase genes. Bacteria isolated from wastewater were classified and their antimicrobial profiles were determined. In total, 252 Gram-negative bacteria carrying various carbapenemase genes were described. Whole-genome sequencing was conducted on 55 selected carbapenemase producing isolates using Oxford Nanopore technology. This study revealed the presence of a significant population of bacteria carrying carbapenemase genes in WWTP, which constitutes a public health problem due to their risk of dissemination to the environment. This emphasizes the usefulness of WWTP monitoring for combating antibiotic resistance. Data revealed the presence of different types of sequences harboring carbapenemase genes, such as <i>bla</i><sub>KPC-2</sub>, <i>bla</i><sub>GES-5</sub>, <i>bla</i><sub>GES-6</sub>, <i>bla</i><sub>IMP-11</sub>, <i>bla</i><sub>IMP-28</sub>, <i>bla</i><sub>OXA-24</sub>, <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>OXA-58</sub>, <i>bla</i><sub>OXA-217</sub>, and <i>bla</i><sub>VIM-2</sub>. Importantly, the presence of the <i>bla</i><sub>KPC-2</sub> gene in wastewater, several months before any clinical case was detected in University Hospital of A Coruña, suggests that wastewater-based epidemiology can be used as an early warning system for the surveillance of antibiotic-resistant bacteria.https://www.mdpi.com/2079-6382/13/2/194antimicrobial resistant bacteriacarbapenemaseGram-negative bacteriawastewater-based epidemiologywhole-genome sequencing<i>bla</i><sub>KPC</sub> |
| spellingShingle | Mohammed Nasser-Ali Pablo Aja-Macaya Kelly Conde-Pérez Noelia Trigo-Tasende Soraya Rumbo-Feal Ana Fernández-González Germán Bou Margarita Poza Juan A. Vallejo Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain Antibiotics antimicrobial resistant bacteria carbapenemase Gram-negative bacteria wastewater-based epidemiology whole-genome sequencing <i>bla</i><sub>KPC</sub> |
| title | Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain |
| title_full | Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain |
| title_fullStr | Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain |
| title_full_unstemmed | Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain |
| title_short | Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain |
| title_sort | emergence of carbapenemase genes in gram negative bacteria isolated from the wastewater treatment plant in a coruna spain |
| topic | antimicrobial resistant bacteria carbapenemase Gram-negative bacteria wastewater-based epidemiology whole-genome sequencing <i>bla</i><sub>KPC</sub> |
| url | https://www.mdpi.com/2079-6382/13/2/194 |
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