| Summary: | <i>Corethrodendron fruticosum</i> is an endemic forage grasses in China with high ecological value. In this study, the complete chloroplast genome of <i>C. fruticosum</i> was sequenced using Illumina paired-end sequencing. The <i>C. fruticosum</i> chloroplast genome was 123,100 bp and comprised 105 genes, including 74 protein-coding genes, 4 rRNA-coding genes, and 27 tRNA-coding genes. The genome had a GC content of 34.53%, with 50 repetitive sequences and 63 simple repeat repetitive sequences that did not contain reverse repeats. The simple repeats included 45 single-nucleotide repeats, which accounted for the highest proportion and primarily comprised A/T repeats. A comparative analysis of <i>C. fruticosum, C. multijugum</i>, and four <i>Hedysarum</i> species revealed that the six genomes were highly conserved, with differentials primarily located in the conserved non-coding regions. Moreover, the <i>accD</i> and <i>clpP</i> genes in the coding regions exhibited high nucleotide variability. Accordingly, these genes may serve as molecular markers for the classification and phylogenetic analysis of <i>Corethrodendron</i> species. Phylogenetic analysis further revealed that <i>C. fruticosum</i> and <i>C. multijugum</i> appeared in different clades than the four <i>Hedysarum</i> species. The newly sequenced chloroplast genome provides further insights into the phylogenetic position of <i>C. fruticosum</i>, which is useful for the classification and identification of <i>Corethrodendron.</i>
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