Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis
<i>Corethrodendron fruticosum</i> is an endemic forage grasses in China with high ecological value. In this study, the complete chloroplast genome of <i>C. fruticosum</i> was sequenced using Illumina paired-end sequencing. The <i>C. fruticosum</i> chloroplast geno...
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MDPI AG
2023-06-01
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| Series: | Genes |
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| author | Tianxiu Niu Chunyu Tian Yanting Yang Qian Liu Lemeng Liu Qibo Tao Zhiyong Li Zinian Wu |
| author_facet | Tianxiu Niu Chunyu Tian Yanting Yang Qian Liu Lemeng Liu Qibo Tao Zhiyong Li Zinian Wu |
| author_sort | Tianxiu Niu |
| collection | DOAJ |
| description | <i>Corethrodendron fruticosum</i> is an endemic forage grasses in China with high ecological value. In this study, the complete chloroplast genome of <i>C. fruticosum</i> was sequenced using Illumina paired-end sequencing. The <i>C. fruticosum</i> chloroplast genome was 123,100 bp and comprised 105 genes, including 74 protein-coding genes, 4 rRNA-coding genes, and 27 tRNA-coding genes. The genome had a GC content of 34.53%, with 50 repetitive sequences and 63 simple repeat repetitive sequences that did not contain reverse repeats. The simple repeats included 45 single-nucleotide repeats, which accounted for the highest proportion and primarily comprised A/T repeats. A comparative analysis of <i>C. fruticosum, C. multijugum</i>, and four <i>Hedysarum</i> species revealed that the six genomes were highly conserved, with differentials primarily located in the conserved non-coding regions. Moreover, the <i>accD</i> and <i>clpP</i> genes in the coding regions exhibited high nucleotide variability. Accordingly, these genes may serve as molecular markers for the classification and phylogenetic analysis of <i>Corethrodendron</i> species. Phylogenetic analysis further revealed that <i>C. fruticosum</i> and <i>C. multijugum</i> appeared in different clades than the four <i>Hedysarum</i> species. The newly sequenced chloroplast genome provides further insights into the phylogenetic position of <i>C. fruticosum</i>, which is useful for the classification and identification of <i>Corethrodendron.</i> |
| first_indexed | 2024-03-11T02:25:18Z |
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| last_indexed | 2024-03-11T02:25:18Z |
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| spelling | doaj.art-556c5fce02b648568f56fc788c8901252023-11-18T10:35:25ZengMDPI AGGenes2073-44252023-06-01146128910.3390/genes14061289Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic AnalysisTianxiu Niu0Chunyu Tian1Yanting Yang2Qian Liu3Lemeng Liu4Qibo Tao5Zhiyong Li6Zinian Wu7Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaKey Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China<i>Corethrodendron fruticosum</i> is an endemic forage grasses in China with high ecological value. In this study, the complete chloroplast genome of <i>C. fruticosum</i> was sequenced using Illumina paired-end sequencing. The <i>C. fruticosum</i> chloroplast genome was 123,100 bp and comprised 105 genes, including 74 protein-coding genes, 4 rRNA-coding genes, and 27 tRNA-coding genes. The genome had a GC content of 34.53%, with 50 repetitive sequences and 63 simple repeat repetitive sequences that did not contain reverse repeats. The simple repeats included 45 single-nucleotide repeats, which accounted for the highest proportion and primarily comprised A/T repeats. A comparative analysis of <i>C. fruticosum, C. multijugum</i>, and four <i>Hedysarum</i> species revealed that the six genomes were highly conserved, with differentials primarily located in the conserved non-coding regions. Moreover, the <i>accD</i> and <i>clpP</i> genes in the coding regions exhibited high nucleotide variability. Accordingly, these genes may serve as molecular markers for the classification and phylogenetic analysis of <i>Corethrodendron</i> species. Phylogenetic analysis further revealed that <i>C. fruticosum</i> and <i>C. multijugum</i> appeared in different clades than the four <i>Hedysarum</i> species. The newly sequenced chloroplast genome provides further insights into the phylogenetic position of <i>C. fruticosum</i>, which is useful for the classification and identification of <i>Corethrodendron.</i>https://www.mdpi.com/2073-4425/14/6/1289<i>Corethrodendron fruticosum</i>chloroplast genomecodon usagerepeat analysisphylogenetic relationship |
| spellingShingle | Tianxiu Niu Chunyu Tian Yanting Yang Qian Liu Lemeng Liu Qibo Tao Zhiyong Li Zinian Wu Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis Genes <i>Corethrodendron fruticosum</i> chloroplast genome codon usage repeat analysis phylogenetic relationship |
| title | Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis |
| title_full | Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis |
| title_fullStr | Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis |
| title_full_unstemmed | Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis |
| title_short | Complete Chloroplast Genome of <i>Corethrodendron fruticosum</i> (Papilionoideae: Fabaceae): Comparative and Phylogenetic Analysis |
| title_sort | complete chloroplast genome of i corethrodendron fruticosum i papilionoideae fabaceae comparative and phylogenetic analysis |
| topic | <i>Corethrodendron fruticosum</i> chloroplast genome codon usage repeat analysis phylogenetic relationship |
| url | https://www.mdpi.com/2073-4425/14/6/1289 |
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