Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls
Parkinson’s disease (PD) is characterized by substantial phenotypic heterogeneity that limits the disease prognosis and patient’s counseling, and complicates the design of further clinical trials. There is an unmet need for the development and validation of biomarkers for the prediction of the disea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1791 |
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| author | Maria Georgoula Panagiotis Ntavaroukas Anastasia Androutsopoulou Georgia Xiromerisiou Fani Kalala Matthaios Speletas Eftihia Asprodini Anna Vasilaki Stamatia Papoutsopoulou |
| author_facet | Maria Georgoula Panagiotis Ntavaroukas Anastasia Androutsopoulou Georgia Xiromerisiou Fani Kalala Matthaios Speletas Eftihia Asprodini Anna Vasilaki Stamatia Papoutsopoulou |
| author_sort | Maria Georgoula |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is characterized by substantial phenotypic heterogeneity that limits the disease prognosis and patient’s counseling, and complicates the design of further clinical trials. There is an unmet need for the development and validation of biomarkers for the prediction of the disease course. In this study, we utilized flow cytometry and in vitro approaches on peripheral blood cells and isolated peripheral blood mononuclear cell (PBMC)-derived macrophages to characterize specific innate immune populations in PD patients versus healthy donors. We found a significantly lower percentage of B lymphocytes and monocyte populations in PD patients. Monocytes in PD patients were characterized by a higher CD40 expression and on-surface expression of the type I membrane glycoprotein sortilin, which showed a trend of negative correlation with the age of the patients. These results were further investigated in vitro on PBMC-derived macrophages, which, in PD patients, showed higher sortilin expression levels compared to cells from healthy donors. The treatment of PD-derived macrophages with oxLDL led to higher foam cell formation compared to healthy donors. In conclusion, our results support the hypothesis that surface sortilin expression levels on human peripheral monocytes may potentially be utilized as a marker of Parkinson’s disease and may segregate the sporadic versus the genetically induced forms of the disease. |
| first_indexed | 2024-03-08T03:54:35Z |
| format | Article |
| id | doaj.art-556ca9d8f57e4c47ab1531c8bda77398 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:54:35Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-556ca9d8f57e4c47ab1531c8bda773982024-02-09T15:14:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01253179110.3390/ijms25031791Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy ControlsMaria Georgoula0Panagiotis Ntavaroukas1Anastasia Androutsopoulou2Georgia Xiromerisiou3Fani Kalala4Matthaios Speletas5Eftihia Asprodini6Anna Vasilaki7Stamatia Papoutsopoulou8Department of Biochemistry & Biotechnology, University of Thessaly, 41500 Larissa, GreeceDepartment of Biochemistry & Biotechnology, University of Thessaly, 41500 Larissa, GreeceDepartment of Biochemistry & Biotechnology, University of Thessaly, 41500 Larissa, GreeceFaculty of Medicine, University of Thessaly, 41500 Larissa, GreeceLaboratory of of Immunology & Histocompatibility, Faculty of Medicine, University of Thessaly, 41500 Larissa, GreeceLaboratory of of Immunology & Histocompatibility, Faculty of Medicine, University of Thessaly, 41500 Larissa, GreeceLaboratory of Clinical Pharmacology, Faculty of Medicine, University of Thessaly, 41500 Larissa, GreeceLaboratory of Pharmacology, Faculty of Medicine, University of Thessaly, 41500 Larissa, GreeceDepartment of Biochemistry & Biotechnology, University of Thessaly, 41500 Larissa, GreeceParkinson’s disease (PD) is characterized by substantial phenotypic heterogeneity that limits the disease prognosis and patient’s counseling, and complicates the design of further clinical trials. There is an unmet need for the development and validation of biomarkers for the prediction of the disease course. In this study, we utilized flow cytometry and in vitro approaches on peripheral blood cells and isolated peripheral blood mononuclear cell (PBMC)-derived macrophages to characterize specific innate immune populations in PD patients versus healthy donors. We found a significantly lower percentage of B lymphocytes and monocyte populations in PD patients. Monocytes in PD patients were characterized by a higher CD40 expression and on-surface expression of the type I membrane glycoprotein sortilin, which showed a trend of negative correlation with the age of the patients. These results were further investigated in vitro on PBMC-derived macrophages, which, in PD patients, showed higher sortilin expression levels compared to cells from healthy donors. The treatment of PD-derived macrophages with oxLDL led to higher foam cell formation compared to healthy donors. In conclusion, our results support the hypothesis that surface sortilin expression levels on human peripheral monocytes may potentially be utilized as a marker of Parkinson’s disease and may segregate the sporadic versus the genetically induced forms of the disease.https://www.mdpi.com/1422-0067/25/3/1791sortilinParkinson’s diseaseperipheral immunitymonocytes |
| spellingShingle | Maria Georgoula Panagiotis Ntavaroukas Anastasia Androutsopoulou Georgia Xiromerisiou Fani Kalala Matthaios Speletas Eftihia Asprodini Anna Vasilaki Stamatia Papoutsopoulou Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls International Journal of Molecular Sciences sortilin Parkinson’s disease peripheral immunity monocytes |
| title | Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls |
| title_full | Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls |
| title_fullStr | Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls |
| title_full_unstemmed | Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls |
| title_short | Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson’s Disease Patients and Healthy Controls |
| title_sort | sortilin expression levels and peripheral immunity a potential biomarker for segregation between parkinson s disease patients and healthy controls |
| topic | sortilin Parkinson’s disease peripheral immunity monocytes |
| url | https://www.mdpi.com/1422-0067/25/3/1791 |
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