The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy
Periodontitis (PD) shows an association with rheumatoid arthritis (RA) and systemic inflammation. Periodontal pathogens, namely <i>Porphyromonas gingivalis</i> and <i>Aggregatibacter actinomycetemcomitans</i>, are proposed to be capable of inducing citrullination of peptides...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/2/193 |
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| author | Emily Davison William Johnston Krystyna Piela Bob T. Rosier Michael Paterson Alex Mira Shauna Culshaw |
| author_facet | Emily Davison William Johnston Krystyna Piela Bob T. Rosier Michael Paterson Alex Mira Shauna Culshaw |
| author_sort | Emily Davison |
| collection | DOAJ |
| description | Periodontitis (PD) shows an association with rheumatoid arthritis (RA) and systemic inflammation. Periodontal pathogens, namely <i>Porphyromonas gingivalis</i> and <i>Aggregatibacter actinomycetemcomitans</i>, are proposed to be capable of inducing citrullination of peptides in the gingiva, inducing the formation of anti-citrullinated protein antibodies (ACPAs) within susceptible hosts. Here, we sought to investigate whether periodontal treatment influenced systemic inflammation and antibody titres to <i>P. gingivalis</i>, <i>A. actinomycetemcomitans</i>, <i>Prevotella intermedia</i> and ACPA in 42 systemically health patients with periodontal disease. Subgingival plaque and serum samples were collected from study participants before (baseline) and 90 days after treatment to analyse the abundance of specific bacteria and evaluate anti-bacterial antibodies, C-reactive protein (CRP), tumour necrosis factor α (TNF-α), interleukin 6 (IL-6) and ACPA in serum. Following treatment, all patients showed reduced periodontal inflammation. Despite observing a weak positive correlation between CRP and IL-6 with periodontal inflammation at baseline, we observed no significant reductions in any indicators of systemic inflammation 90 days after treatment. In contrast, anti-<i>P. gingivalis</i> IgG significantly reduced post-treatment (<i>p</i> < 0.001, Wilcoxon signed rank test), although no changes were observed for other antibody titres. Patients who had detectable <i>P. gingivalis</i> in subgingival plaques had significantly higher anti-<i>P. gingivalis</i> IgG and ACPA titres, suggesting a potential association between <i>P. gingivalis</i> colonisation and systemic antibody titres. |
| first_indexed | 2024-03-09T04:50:53Z |
| format | Article |
| id | doaj.art-556de95182794d549a8ea7587577195e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T04:50:53Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-556de95182794d549a8ea7587577195e2023-12-03T13:10:42ZengMDPI AGPathogens2076-08172021-02-0110219310.3390/pathogens10020193The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal TherapyEmily Davison0William Johnston1Krystyna Piela2Bob T. Rosier3Michael Paterson4Alex Mira5Shauna Culshaw6Oral Sciences, College of Medical, Veterinary and Life Sciences, Dental School, University of Glasgow, Glasgow G12 8QQ, UKOral Sciences, College of Medical, Veterinary and Life Sciences, Dental School, University of Glasgow, Glasgow G12 8QQ, UKOral Sciences, College of Medical, Veterinary and Life Sciences, Dental School, University of Glasgow, Glasgow G12 8QQ, UKThe Foundation for the Promotion of Health and Biomedical Research (FISABIO), 46020 Valencia, SpainOral Sciences, College of Medical, Veterinary and Life Sciences, Dental School, University of Glasgow, Glasgow G12 8QQ, UKThe Foundation for the Promotion of Health and Biomedical Research (FISABIO), 46020 Valencia, SpainOral Sciences, College of Medical, Veterinary and Life Sciences, Dental School, University of Glasgow, Glasgow G12 8QQ, UKPeriodontitis (PD) shows an association with rheumatoid arthritis (RA) and systemic inflammation. Periodontal pathogens, namely <i>Porphyromonas gingivalis</i> and <i>Aggregatibacter actinomycetemcomitans</i>, are proposed to be capable of inducing citrullination of peptides in the gingiva, inducing the formation of anti-citrullinated protein antibodies (ACPAs) within susceptible hosts. Here, we sought to investigate whether periodontal treatment influenced systemic inflammation and antibody titres to <i>P. gingivalis</i>, <i>A. actinomycetemcomitans</i>, <i>Prevotella intermedia</i> and ACPA in 42 systemically health patients with periodontal disease. Subgingival plaque and serum samples were collected from study participants before (baseline) and 90 days after treatment to analyse the abundance of specific bacteria and evaluate anti-bacterial antibodies, C-reactive protein (CRP), tumour necrosis factor α (TNF-α), interleukin 6 (IL-6) and ACPA in serum. Following treatment, all patients showed reduced periodontal inflammation. Despite observing a weak positive correlation between CRP and IL-6 with periodontal inflammation at baseline, we observed no significant reductions in any indicators of systemic inflammation 90 days after treatment. In contrast, anti-<i>P. gingivalis</i> IgG significantly reduced post-treatment (<i>p</i> < 0.001, Wilcoxon signed rank test), although no changes were observed for other antibody titres. Patients who had detectable <i>P. gingivalis</i> in subgingival plaques had significantly higher anti-<i>P. gingivalis</i> IgG and ACPA titres, suggesting a potential association between <i>P. gingivalis</i> colonisation and systemic antibody titres.https://www.mdpi.com/2076-0817/10/2/193periodontitisperiodontal therapy<i>Porphyromonas gingivalis</i><i>Aggregatibacter actinomycetemcomitans</i>anti-citrullinated protein antibodiessubgingival plaque |
| spellingShingle | Emily Davison William Johnston Krystyna Piela Bob T. Rosier Michael Paterson Alex Mira Shauna Culshaw The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy Pathogens periodontitis periodontal therapy <i>Porphyromonas gingivalis</i> <i>Aggregatibacter actinomycetemcomitans</i> anti-citrullinated protein antibodies subgingival plaque |
| title | The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy |
| title_full | The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy |
| title_fullStr | The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy |
| title_full_unstemmed | The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy |
| title_short | The Subgingival Plaque Microbiome, Systemic Antibodies against Bacteria and Citrullinated Proteins following Periodontal Therapy |
| title_sort | subgingival plaque microbiome systemic antibodies against bacteria and citrullinated proteins following periodontal therapy |
| topic | periodontitis periodontal therapy <i>Porphyromonas gingivalis</i> <i>Aggregatibacter actinomycetemcomitans</i> anti-citrullinated protein antibodies subgingival plaque |
| url | https://www.mdpi.com/2076-0817/10/2/193 |
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