Endocardium gives rise to blood cells in zebrafish embryos
Summary: Previous studies have suggested that the endocardium contributes to hematopoiesis in murine embryos, although definitive evidence to demonstrate the hematopoietic potential of the endocardium is still missing. Here, we use a zebrafish embryonic model to test the emergence of hematopoietic p...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724000640 |
| _version_ | 1827338910819155968 |
|---|---|
| author | Suman Gurung Nicole K. Restrepo Saulius Sumanas |
| author_facet | Suman Gurung Nicole K. Restrepo Saulius Sumanas |
| author_sort | Suman Gurung |
| collection | DOAJ |
| description | Summary: Previous studies have suggested that the endocardium contributes to hematopoiesis in murine embryos, although definitive evidence to demonstrate the hematopoietic potential of the endocardium is still missing. Here, we use a zebrafish embryonic model to test the emergence of hematopoietic progenitors from the endocardium. By using a combination of expression analysis, time-lapse imaging, and lineage-tracing approaches, we demonstrate that myeloid cells emerge from the endocardium in zebrafish embryos. Inhibition of Etv2/Etsrp or Scl/Tal1, two known master regulators of hematopoiesis and vasculogenesis, does not affect the emergence of endocardial-derived myeloid cells, while inhibition of Hedgehog signaling results in their reduction. Single-cell RNA sequencing analysis followed by experimental validation suggests that the endocardium is the major source of neutrophilic granulocytes. These findings will promote our understanding of alternative mechanisms involved in hematopoiesis, which are likely to be conserved between zebrafish and mammalian embryos. |
| first_indexed | 2024-03-07T19:42:26Z |
| format | Article |
| id | doaj.art-556f179075024a8c845919d12f605db5 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-07T19:42:26Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-556f179075024a8c845919d12f605db52024-02-29T05:18:48ZengElsevierCell Reports2211-12472024-02-01432113736Endocardium gives rise to blood cells in zebrafish embryosSuman Gurung0Nicole K. Restrepo1Saulius Sumanas2Department of Pathology and Cell Biology, USF Health Heart Institute, University of South Florida, Tampa, FL 33602, USA; Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA; Department of Pathology, Advanced Diagnostics Laboratories, Moffitt Cancer Center, Tampa, FL 33612, USADepartment of Pathology and Cell Biology, USF Health Heart Institute, University of South Florida, Tampa, FL 33602, USADepartment of Pathology and Cell Biology, USF Health Heart Institute, University of South Florida, Tampa, FL 33602, USA; Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USA; University of Cincinnati College of Medicine, Department of Pediatrics, Cincinnati, OH 45229, USA; Corresponding authorSummary: Previous studies have suggested that the endocardium contributes to hematopoiesis in murine embryos, although definitive evidence to demonstrate the hematopoietic potential of the endocardium is still missing. Here, we use a zebrafish embryonic model to test the emergence of hematopoietic progenitors from the endocardium. By using a combination of expression analysis, time-lapse imaging, and lineage-tracing approaches, we demonstrate that myeloid cells emerge from the endocardium in zebrafish embryos. Inhibition of Etv2/Etsrp or Scl/Tal1, two known master regulators of hematopoiesis and vasculogenesis, does not affect the emergence of endocardial-derived myeloid cells, while inhibition of Hedgehog signaling results in their reduction. Single-cell RNA sequencing analysis followed by experimental validation suggests that the endocardium is the major source of neutrophilic granulocytes. These findings will promote our understanding of alternative mechanisms involved in hematopoiesis, which are likely to be conserved between zebrafish and mammalian embryos.http://www.sciencedirect.com/science/article/pii/S2211124724000640CP: Developmental biologyCP: Immunology |
| spellingShingle | Suman Gurung Nicole K. Restrepo Saulius Sumanas Endocardium gives rise to blood cells in zebrafish embryos Cell Reports CP: Developmental biology CP: Immunology |
| title | Endocardium gives rise to blood cells in zebrafish embryos |
| title_full | Endocardium gives rise to blood cells in zebrafish embryos |
| title_fullStr | Endocardium gives rise to blood cells in zebrafish embryos |
| title_full_unstemmed | Endocardium gives rise to blood cells in zebrafish embryos |
| title_short | Endocardium gives rise to blood cells in zebrafish embryos |
| title_sort | endocardium gives rise to blood cells in zebrafish embryos |
| topic | CP: Developmental biology CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724000640 |
| work_keys_str_mv | AT sumangurung endocardiumgivesrisetobloodcellsinzebrafishembryos AT nicolekrestrepo endocardiumgivesrisetobloodcellsinzebrafishembryos AT sauliussumanas endocardiumgivesrisetobloodcellsinzebrafishembryos |