Transfection reagent artefact likely accounts for some reports of extracellular vesicle function

Transfection reagent artefact likely accounts for some reports of extracellular vesicle function

Abstract Extracellular vesicles (EV) are important mediators of cell communication and physiology. EVs are frequently investigated by transiently transfecting cells with plasmid DNA to produce EVs modified with protein(s) or nucleic acid(s) of interest. DNA‐transfection reagent complexes (DTC) are a...

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Bibliographic Details
Main Authors: Russell E. McConnell, Madeleine Youniss, Bhargavee Gnanasambandam, Palak Shah, Wei Zhang, Jonathan D. Finn
Format: Article
Language:English
Published: Wiley 2022-10-01
Series:Journal of Extracellular Vesicles
Subjects:
artefact
Cre
delivery
exosome
extracellular vesicle
purification
Online Access:https://doi.org/10.1002/jev2.12253

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